Self-cycling fermentation for 1,3-propanediol production: Comparative evaluation of metabolite flux in cell recycling, simple batch and continuous processes using Lactobacillus brevis N1E9.3.3 strain

Narisetty Vivek, T. V. Aswathi, Petit Riff Sven, Ashok Pandey, Parameswaran Binod

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The microbial conversion of biodiesel derived crude glycerol to 1,3-propanediol (1,3-PDO) has attained high industrial value due to the broad range of applications as a monomer (1,3-PDO) in textile, cosmetic and polymer industries. This fine chemical through biological production addressed several limitations of the chemical process like high temperatures, pressure and expensive catalysts. In this study 1,3-propanediol production was achieved from a non-pathogenic lactic acid bacterial strain Lactobacillus brevis N1E9.3.3 in suspended and immobilized form under batch, sequential batch and continuous modes of fermentation. The microorganism was immobilized in polyurethane foam cubes. The effects of initial glycerol concentration on suspended and immobilized cells were investigated in stirred tank reactor. The maximum 1,3-PDO titers of 51.5 g1,3-PDO/l and 42.59 g1,3-PDO/l with a yield of 0.64 g1.3-PDO/gGlycerol and 0.53 g1.3-PDO/gGlycerol using suspended and immobilized cells respectively was observed in batch fermentation with an initial glycerol concentration of 80 g/l. In repeated batch (self-cycling) fermentation 78.3 g/l 1,3-PDO with 0.65 g1.3-PDO/gGlycerol yield and 0.65 g/l/h productivity was observed at the end of 5th cycle. A constant yield between 0.3–0.65 g1.3-PDO/gGlycerol was observed with cell recycling using suspended and immobilized cells. The highest productivity of 1.735 g1,3-PDO/l/h was observed in chemostat with immobilized cells.

Original languageEnglish
Pages (from-to)110-119
Number of pages10
JournalJournal of Biotechnology
Volume259
DOIs
Publication statusPublished - 10-10-2017
Externally publishedYes

Fingerprint

Lactobacillus brevis
Metabolites
Immobilized Cells
Fermentation
Recycling
Glycerol
Cells
Fluxes
Productivity
Chemostats
Chemical Phenomena
Cosmetics
Lactic acid
Biodiesel
Microorganisms
Biofuels
Textiles
Polyurethanes
Foams
Monomers

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

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title = "Self-cycling fermentation for 1,3-propanediol production: Comparative evaluation of metabolite flux in cell recycling, simple batch and continuous processes using Lactobacillus brevis N1E9.3.3 strain",
abstract = "The microbial conversion of biodiesel derived crude glycerol to 1,3-propanediol (1,3-PDO) has attained high industrial value due to the broad range of applications as a monomer (1,3-PDO) in textile, cosmetic and polymer industries. This fine chemical through biological production addressed several limitations of the chemical process like high temperatures, pressure and expensive catalysts. In this study 1,3-propanediol production was achieved from a non-pathogenic lactic acid bacterial strain Lactobacillus brevis N1E9.3.3 in suspended and immobilized form under batch, sequential batch and continuous modes of fermentation. The microorganism was immobilized in polyurethane foam cubes. The effects of initial glycerol concentration on suspended and immobilized cells were investigated in stirred tank reactor. The maximum 1,3-PDO titers of 51.5 g1,3-PDO/l and 42.59 g1,3-PDO/l with a yield of 0.64 g1.3-PDO/gGlycerol and 0.53 g1.3-PDO/gGlycerol using suspended and immobilized cells respectively was observed in batch fermentation with an initial glycerol concentration of 80 g/l. In repeated batch (self-cycling) fermentation 78.3 g/l 1,3-PDO with 0.65 g1.3-PDO/gGlycerol yield and 0.65 g/l/h productivity was observed at the end of 5th cycle. A constant yield between 0.3–0.65 g1.3-PDO/gGlycerol was observed with cell recycling using suspended and immobilized cells. The highest productivity of 1.735 g1,3-PDO/l/h was observed in chemostat with immobilized cells.",
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Self-cycling fermentation for 1,3-propanediol production : Comparative evaluation of metabolite flux in cell recycling, simple batch and continuous processes using Lactobacillus brevis N1E9.3.3 strain. / Vivek, Narisetty; Aswathi, T. V.; Sven, Petit Riff; Pandey, Ashok; Binod, Parameswaran.

In: Journal of Biotechnology, Vol. 259, 10.10.2017, p. 110-119.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Self-cycling fermentation for 1,3-propanediol production

T2 - Comparative evaluation of metabolite flux in cell recycling, simple batch and continuous processes using Lactobacillus brevis N1E9.3.3 strain

AU - Vivek, Narisetty

AU - Aswathi, T. V.

AU - Sven, Petit Riff

AU - Pandey, Ashok

AU - Binod, Parameswaran

PY - 2017/10/10

Y1 - 2017/10/10

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