Sensitivity of lymphocytes of patients with fibroadenoma and breast cancer to gamma-rays as evaluated by chromosomal aberrations

Usha Kumari, G. K. Bhat

Research output: Contribution to journalArticle

Abstract

Radiosensitivity as determined by chromosomal aberrations in lymphocytes of patients suffering from fibroadenoma (n=31) and breast cancer (n=41) and was compared with the data from those of healthy individuals (n=10). Lymphocytes from healthy control set of individuals were exposed at G0 stage to different doses (0.5 Gy, 1.0 Gy, 2.0 Gy and 4.0 Gy) of γ-rays. As over 60% cells remained viable at a dose of 2 Gy, this dose was selected for further experimentation . Lymphocytes were cultured for 48 hours to obtain data from first mitotic metaphases. Frequencies of chromosomal aberrations namely acentric fragments, double minutes, dicentrics and rings were recorded. Lymphocytes of patients with fibroadenoma or breast cancer exhibited higher radiation sensitivity compared to lymphocytes from healthy individuals. Lymphocytes of patients with different stages of breast cancer showed an increase in the frequency of chromosomal aberrations from stage I to IV.

Original languageEnglish
Pages (from-to)351-356
Number of pages6
JournalInternational Journal of Human Genetics
Volume8
Issue number4
DOIs
Publication statusPublished - 01-01-2008

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Fibroadenoma
Gamma Rays
Chromosome Aberrations
Lymphocytes
Breast Neoplasms
Radiation Tolerance
Metaphase

All Science Journal Classification (ASJC) codes

  • Genetics
  • Genetics(clinical)

Cite this

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