Serum Adenosine deaminase and malondialdehyde as determinants of extent of myocardial reperfusion injury

Y. Dhanunjaya, Aparna R. Bitla, D. Rajashekhar, M. M. Suchitra, P. V L N Srinivasa Rao

Research output: Contribution to journalArticle

Abstract

Background & Objectives: Early reperfusion of the ischemic myocardium is required for tissue survival which itself can induce free radical mediated injury to the myocardium, thereby reducing the beneficial effects of myocardial reperfusion. The currently used markers of reperfusion injury are invasive and costly. A biochemical marker could be more useful particularly because it is non-invasive and also cost effective. Adenosine, the substrate for Adenosine Deaminase (ADA) can inhibit the invasion of neutrophils to attenuate the ischemia/reperfusion injury. Inhibition of adenosine deaminase has been shown to prevent free radical mediated injury in the post-ischemic heart in animal models. The other parameter which could determine the rate of oxidative stress is Malondialdehyde (MDA), and now it is considered as marker of lipid peroxidation. Therefore we measured plasma levels of ADA & MDA in post MI patients. Methods: In the present comparative study we determined plasma levels of adenosine deaminase and malondialdehyde in thirty patients with acute myocardial infarction along with age & sex matched healthy individuals. Results: Serum levels of MDA and ADA were found to be significantly elevated in AMI patients (p < 0.0001 & < 0.001 respectively) when compared to healthy controls. Conclusion: Our findings point towards the possible role of these parameters in reperfusion injury and can thus be an easy and noninvasive way to assess reperfusion injury and initiate treatment.

Original languageEnglish
Pages (from-to)411-414
Number of pages4
JournalBiomedicine
Volume31
Issue number3
Publication statusPublished - 07-2011

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Myocardial Reperfusion Injury
Adenosine Deaminase
Malondialdehyde
Reperfusion Injury
Serum
Free Radicals
Myocardium
Plasmas
Tissue Survival
Myocardial Reperfusion
Oxidative stress
Wounds and Injuries
Adenosine
Lipid Peroxidation
Reperfusion
Animals
Oxidative Stress
Neutrophils
Animal Models
Biomarkers

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Dhanunjaya, Y. ; Bitla, Aparna R. ; Rajashekhar, D. ; Suchitra, M. M. ; Rao, P. V L N Srinivasa. / Serum Adenosine deaminase and malondialdehyde as determinants of extent of myocardial reperfusion injury. In: Biomedicine. 2011 ; Vol. 31, No. 3. pp. 411-414.
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Dhanunjaya, Y, Bitla, AR, Rajashekhar, D, Suchitra, MM & Rao, PVLNS 2011, 'Serum Adenosine deaminase and malondialdehyde as determinants of extent of myocardial reperfusion injury', Biomedicine, vol. 31, no. 3, pp. 411-414.

Serum Adenosine deaminase and malondialdehyde as determinants of extent of myocardial reperfusion injury. / Dhanunjaya, Y.; Bitla, Aparna R.; Rajashekhar, D.; Suchitra, M. M.; Rao, P. V L N Srinivasa.

In: Biomedicine, Vol. 31, No. 3, 07.2011, p. 411-414.

Research output: Contribution to journalArticle

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AU - Dhanunjaya, Y.

AU - Bitla, Aparna R.

AU - Rajashekhar, D.

AU - Suchitra, M. M.

AU - Rao, P. V L N Srinivasa

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N2 - Background & Objectives: Early reperfusion of the ischemic myocardium is required for tissue survival which itself can induce free radical mediated injury to the myocardium, thereby reducing the beneficial effects of myocardial reperfusion. The currently used markers of reperfusion injury are invasive and costly. A biochemical marker could be more useful particularly because it is non-invasive and also cost effective. Adenosine, the substrate for Adenosine Deaminase (ADA) can inhibit the invasion of neutrophils to attenuate the ischemia/reperfusion injury. Inhibition of adenosine deaminase has been shown to prevent free radical mediated injury in the post-ischemic heart in animal models. The other parameter which could determine the rate of oxidative stress is Malondialdehyde (MDA), and now it is considered as marker of lipid peroxidation. Therefore we measured plasma levels of ADA & MDA in post MI patients. Methods: In the present comparative study we determined plasma levels of adenosine deaminase and malondialdehyde in thirty patients with acute myocardial infarction along with age & sex matched healthy individuals. Results: Serum levels of MDA and ADA were found to be significantly elevated in AMI patients (p < 0.0001 & < 0.001 respectively) when compared to healthy controls. Conclusion: Our findings point towards the possible role of these parameters in reperfusion injury and can thus be an easy and noninvasive way to assess reperfusion injury and initiate treatment.

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