Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine

Maya Roche, G. Kusumanjali, G. Chinnapu Reddy, A. S. Kanagasabhapathy, Pragna Rao

Research output: Contribution to journalArticle

Abstract

Cyclosporine has been reported to function as an inhibitor of the chymotrypsin like activity of proteasome. We hypothesized that the administration of an exogenous proteinase inhibitor may affect the activities of the naturally occurring serum anti proteinases. The aim of this study was to observe the pattern of alteration of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) activities in renal transplant patients receiving the immunosuppressive drug, cyclosporine. Patients (97) who had received a single renal allograft were inducted into the study. Subjects were on a twice-daily dosage of cyclosporine capsules. Trough (Co) and two-hour post dose (C 2) cyclosporine levels were regularly estimated and all patients had stable creatinine levels. In 5 newly transplanted patients, antiproteinase activities were estimated weekly over a 4-week period as their cyclosporine doses were gradually tapered. Average serum activities of ACT and AMG in the transplant group were significantly less than in the control group (p<0.002 and p<0.003 respectively). AT and ACT activities fell gradually over 4 weeks. AMG activities showed a biphasic pattern, initially falling by almost 50% in the second week, increasing marginally in the third week and decreasing to less than 50% of the activities observed in the first week. Serum antiproteinase activities of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) were found to be altered in renal transplant patients receiving cyclosporine.

Original languageEnglish
Pages (from-to)63-66
Number of pages4
JournalIndian Journal of Clinical Biochemistry
Volume21
Issue number2
DOIs
Publication statusPublished - 2006

Fingerprint

alpha-Macroglobulins
Cyclosporine
Transplants
alpha 1-Antichymotrypsin
Serum
alpha 1-Antitrypsin
Kidney
Peptide Hydrolases
Therapeutics
Accidental Falls
Chymotrypsin
Proteasome Endopeptidase Complex
Immunosuppressive Agents
Capsules
Allografts
Creatinine
Control Groups
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Roche, Maya ; Kusumanjali, G. ; Chinnapu Reddy, G. ; Kanagasabhapathy, A. S. ; Rao, Pragna. / Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine. In: Indian Journal of Clinical Biochemistry. 2006 ; Vol. 21, No. 2. pp. 63-66.
@article{edf45baeefd84e32a839848ce92b76e4,
title = "Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine",
abstract = "Cyclosporine has been reported to function as an inhibitor of the chymotrypsin like activity of proteasome. We hypothesized that the administration of an exogenous proteinase inhibitor may affect the activities of the naturally occurring serum anti proteinases. The aim of this study was to observe the pattern of alteration of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) activities in renal transplant patients receiving the immunosuppressive drug, cyclosporine. Patients (97) who had received a single renal allograft were inducted into the study. Subjects were on a twice-daily dosage of cyclosporine capsules. Trough (Co) and two-hour post dose (C 2) cyclosporine levels were regularly estimated and all patients had stable creatinine levels. In 5 newly transplanted patients, antiproteinase activities were estimated weekly over a 4-week period as their cyclosporine doses were gradually tapered. Average serum activities of ACT and AMG in the transplant group were significantly less than in the control group (p<0.002 and p<0.003 respectively). AT and ACT activities fell gradually over 4 weeks. AMG activities showed a biphasic pattern, initially falling by almost 50{\%} in the second week, increasing marginally in the third week and decreasing to less than 50{\%} of the activities observed in the first week. Serum antiproteinase activities of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) were found to be altered in renal transplant patients receiving cyclosporine.",
author = "Maya Roche and G. Kusumanjali and {Chinnapu Reddy}, G. and Kanagasabhapathy, {A. S.} and Pragna Rao",
year = "2006",
doi = "10.1007/BF02912914",
language = "English",
volume = "21",
pages = "63--66",
journal = "Indian Journal of Clinical Biochemistry",
issn = "0970-1915",
publisher = "Springer India",
number = "2",

}

Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine. / Roche, Maya; Kusumanjali, G.; Chinnapu Reddy, G.; Kanagasabhapathy, A. S.; Rao, Pragna.

In: Indian Journal of Clinical Biochemistry, Vol. 21, No. 2, 2006, p. 63-66.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Serum alpha-2-macroglobulin, antitrypsin and antichymotrypsin activities in patients receiving treatment with cyclosporine

AU - Roche, Maya

AU - Kusumanjali, G.

AU - Chinnapu Reddy, G.

AU - Kanagasabhapathy, A. S.

AU - Rao, Pragna

PY - 2006

Y1 - 2006

N2 - Cyclosporine has been reported to function as an inhibitor of the chymotrypsin like activity of proteasome. We hypothesized that the administration of an exogenous proteinase inhibitor may affect the activities of the naturally occurring serum anti proteinases. The aim of this study was to observe the pattern of alteration of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) activities in renal transplant patients receiving the immunosuppressive drug, cyclosporine. Patients (97) who had received a single renal allograft were inducted into the study. Subjects were on a twice-daily dosage of cyclosporine capsules. Trough (Co) and two-hour post dose (C 2) cyclosporine levels were regularly estimated and all patients had stable creatinine levels. In 5 newly transplanted patients, antiproteinase activities were estimated weekly over a 4-week period as their cyclosporine doses were gradually tapered. Average serum activities of ACT and AMG in the transplant group were significantly less than in the control group (p<0.002 and p<0.003 respectively). AT and ACT activities fell gradually over 4 weeks. AMG activities showed a biphasic pattern, initially falling by almost 50% in the second week, increasing marginally in the third week and decreasing to less than 50% of the activities observed in the first week. Serum antiproteinase activities of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) were found to be altered in renal transplant patients receiving cyclosporine.

AB - Cyclosporine has been reported to function as an inhibitor of the chymotrypsin like activity of proteasome. We hypothesized that the administration of an exogenous proteinase inhibitor may affect the activities of the naturally occurring serum anti proteinases. The aim of this study was to observe the pattern of alteration of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) activities in renal transplant patients receiving the immunosuppressive drug, cyclosporine. Patients (97) who had received a single renal allograft were inducted into the study. Subjects were on a twice-daily dosage of cyclosporine capsules. Trough (Co) and two-hour post dose (C 2) cyclosporine levels were regularly estimated and all patients had stable creatinine levels. In 5 newly transplanted patients, antiproteinase activities were estimated weekly over a 4-week period as their cyclosporine doses were gradually tapered. Average serum activities of ACT and AMG in the transplant group were significantly less than in the control group (p<0.002 and p<0.003 respectively). AT and ACT activities fell gradually over 4 weeks. AMG activities showed a biphasic pattern, initially falling by almost 50% in the second week, increasing marginally in the third week and decreasing to less than 50% of the activities observed in the first week. Serum antiproteinase activities of serum alpha 2 macroglobulin (AMG), alpha 1- antitrypsin (AT) and alpha 1-antichymotrypsin (ACT) were found to be altered in renal transplant patients receiving cyclosporine.

UR - http://www.scopus.com/inward/record.url?scp=33751048846&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33751048846&partnerID=8YFLogxK

U2 - 10.1007/BF02912914

DO - 10.1007/BF02912914

M3 - Article

VL - 21

SP - 63

EP - 66

JO - Indian Journal of Clinical Biochemistry

JF - Indian Journal of Clinical Biochemistry

SN - 0970-1915

IS - 2

ER -