Simultaneous determination of linezolid and levamisole hydrochloride in a fixed dose combination

Lalit Kumar, Yugvijay Singh Yadav, Mahalaxmi Rathnanand

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Background: The combination of antibiotics with an immune-stimulant can be used for the prevention of antimicrobial resistance. Multilayer tablets of linezolid and levamisole hydrochloride can be a solution of this problem. Objective: Aim of the present study was to High Performance Liquid Chromatography method development and validation as per the International Council for Harmonisation guidelines for analysis of both the drugs simultaneously in a single unit dosage form. Methods: Ammonium acetate and acetonitrile in the ratio 65:35 (%v/v) was used as mobile phase. Ultra violet detection was carried out at 236 nm and flow rate was kept as 0.9 mL/min. Results: Linezolid and levamisole hydrochloride were found at 4.61 min and 6.45 min retention times, respectively. Conclusion: The study confirmed that the present method can be used for routine simultaneous analysis of linezolid and levamisole hydrochloride.

Original languageEnglish
Pages (from-to)613-619
Number of pages7
JournalIndian Journal of Pharmaceutical Education and Research
Volume51
Issue number4
DOIs
Publication statusPublished - 01-10-2017

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Linezolid
Levamisole
Dosage Forms
Tablets
High Pressure Liquid Chromatography
Guidelines
Anti-Bacterial Agents
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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abstract = "Background: The combination of antibiotics with an immune-stimulant can be used for the prevention of antimicrobial resistance. Multilayer tablets of linezolid and levamisole hydrochloride can be a solution of this problem. Objective: Aim of the present study was to High Performance Liquid Chromatography method development and validation as per the International Council for Harmonisation guidelines for analysis of both the drugs simultaneously in a single unit dosage form. Methods: Ammonium acetate and acetonitrile in the ratio 65:35 ({\%}v/v) was used as mobile phase. Ultra violet detection was carried out at 236 nm and flow rate was kept as 0.9 mL/min. Results: Linezolid and levamisole hydrochloride were found at 4.61 min and 6.45 min retention times, respectively. Conclusion: The study confirmed that the present method can be used for routine simultaneous analysis of linezolid and levamisole hydrochloride.",
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Simultaneous determination of linezolid and levamisole hydrochloride in a fixed dose combination. / Kumar, Lalit; Yadav, Yugvijay Singh; Rathnanand, Mahalaxmi.

In: Indian Journal of Pharmaceutical Education and Research, Vol. 51, No. 4, 01.10.2017, p. 613-619.

Research output: Contribution to journalArticle

TY - JOUR

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AU - Yadav, Yugvijay Singh

AU - Rathnanand, Mahalaxmi

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AB - Background: The combination of antibiotics with an immune-stimulant can be used for the prevention of antimicrobial resistance. Multilayer tablets of linezolid and levamisole hydrochloride can be a solution of this problem. Objective: Aim of the present study was to High Performance Liquid Chromatography method development and validation as per the International Council for Harmonisation guidelines for analysis of both the drugs simultaneously in a single unit dosage form. Methods: Ammonium acetate and acetonitrile in the ratio 65:35 (%v/v) was used as mobile phase. Ultra violet detection was carried out at 236 nm and flow rate was kept as 0.9 mL/min. Results: Linezolid and levamisole hydrochloride were found at 4.61 min and 6.45 min retention times, respectively. Conclusion: The study confirmed that the present method can be used for routine simultaneous analysis of linezolid and levamisole hydrochloride.

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