Simultaneous RP-HPLC estimation of tizanidine and valdecoxib in tablets

G. Subramanian, M. Faisal, V. Bhat, A. Kumar, N. Udupa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A reverse phase liquid chromatographic method was developed for the simultaneous estimation of tizanidine and valdecoxib in tablets. This method is based on using a Hypersil BDS C18 column using a mobile phase of 10 mM octane sulphonic acid sodium salt and 0.3% triethylamine (pH adjusted to 3.5±0.1 with orthophosphoric acid) and acetonitrile in the ratio of 70:30 v/v. Rofecoxib was used as an internal standard. The retention time of tizanidine, valdecoxib, and rofecoxib were 3.15, 10.92, and 16.24 min respectively. The method was found to be linear (correlation coefficient r>0.999), precise (Residual standard deviation: 0.62% for tizanidine, 0.86% for valdecoxib), accurate (overall average recovery yields: 98.7% for tizanidine, 99.2% for valdecoxib), and selective.

Original languageEnglish
Pages (from-to)391-393
Number of pages3
JournalIndian Journal of Pharmaceutical Sciences
Volume68
Issue number3
Publication statusPublished - 01-02-2006

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Tablets
High Pressure Liquid Chromatography
Sulfonic Acids
Salts
Sodium
valdecoxib
tizanidine
rofecoxib

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

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abstract = "A reverse phase liquid chromatographic method was developed for the simultaneous estimation of tizanidine and valdecoxib in tablets. This method is based on using a Hypersil BDS C18 column using a mobile phase of 10 mM octane sulphonic acid sodium salt and 0.3{\%} triethylamine (pH adjusted to 3.5±0.1 with orthophosphoric acid) and acetonitrile in the ratio of 70:30 v/v. Rofecoxib was used as an internal standard. The retention time of tizanidine, valdecoxib, and rofecoxib were 3.15, 10.92, and 16.24 min respectively. The method was found to be linear (correlation coefficient r>0.999), precise (Residual standard deviation: 0.62{\%} for tizanidine, 0.86{\%} for valdecoxib), accurate (overall average recovery yields: 98.7{\%} for tizanidine, 99.2{\%} for valdecoxib), and selective.",
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Simultaneous RP-HPLC estimation of tizanidine and valdecoxib in tablets. / Subramanian, G.; Faisal, M.; Bhat, V.; Kumar, A.; Udupa, N.

In: Indian Journal of Pharmaceutical Sciences, Vol. 68, No. 3, 01.02.2006, p. 391-393.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Simultaneous RP-HPLC estimation of tizanidine and valdecoxib in tablets

AU - Subramanian, G.

AU - Faisal, M.

AU - Bhat, V.

AU - Kumar, A.

AU - Udupa, N.

PY - 2006/2/1

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N2 - A reverse phase liquid chromatographic method was developed for the simultaneous estimation of tizanidine and valdecoxib in tablets. This method is based on using a Hypersil BDS C18 column using a mobile phase of 10 mM octane sulphonic acid sodium salt and 0.3% triethylamine (pH adjusted to 3.5±0.1 with orthophosphoric acid) and acetonitrile in the ratio of 70:30 v/v. Rofecoxib was used as an internal standard. The retention time of tizanidine, valdecoxib, and rofecoxib were 3.15, 10.92, and 16.24 min respectively. The method was found to be linear (correlation coefficient r>0.999), precise (Residual standard deviation: 0.62% for tizanidine, 0.86% for valdecoxib), accurate (overall average recovery yields: 98.7% for tizanidine, 99.2% for valdecoxib), and selective.

AB - A reverse phase liquid chromatographic method was developed for the simultaneous estimation of tizanidine and valdecoxib in tablets. This method is based on using a Hypersil BDS C18 column using a mobile phase of 10 mM octane sulphonic acid sodium salt and 0.3% triethylamine (pH adjusted to 3.5±0.1 with orthophosphoric acid) and acetonitrile in the ratio of 70:30 v/v. Rofecoxib was used as an internal standard. The retention time of tizanidine, valdecoxib, and rofecoxib were 3.15, 10.92, and 16.24 min respectively. The method was found to be linear (correlation coefficient r>0.999), precise (Residual standard deviation: 0.62% for tizanidine, 0.86% for valdecoxib), accurate (overall average recovery yields: 98.7% for tizanidine, 99.2% for valdecoxib), and selective.

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