Single dose vaccine delivery system of tetanus toxoid formulation based on chitosan microspheres

B. Dineshkumar, S. A. Dhanaraj, K. Santhi, P. Vijayan, Raghu Chandrasekhar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: Biodegradable polymers such as Chitosan or PLGA has been used for sustain release of vaccines for prolonged period. Purpose of the study: To develop single dose vaccine delivery system of tetanus toxoid using chitosan microspheres. Different batches, 1%, 2% and 3% of chitosan microspheres (CM) were prepared by emulsion cross-linking method using glutaradehyde saturated toluene (GST). The tetanus toxoid (TT) was loaded with 1%, 2% and 3% of CM by adsorption method. Percentage of TT absorbed in 1%, 2% and 3% CM were determined by Limes flocculation method. In vitro release of adsorbed TT from 1%, 2% and 3% CM were performed with PBS (pH 7.4) and evaluated by ELISA assay using purified guinea pig IgG by affinity chromatography. Results: The average particle size of 1%, 2% and 3% of CM were determined by optical microscopic method and particle size was found to be 30μm, 31μm, and 35μm respectively. Percentage of TT adsorbed in 1%, 2% and 3% CM were found to be 71%, 80% and 76% respectively by Limes flocculation method. In vitro studies showed that different batches (1%, 2% and 3%) of CM showed cumulative percentage release of TT 74.09%, 89.31%, and 80.23% respectively for 50 days. Among three batches of microspheres, 2% of chitosan microspheres showed better consistent and sustained release of TT for 50 days. Conclusions: This study suggested that 2% of chitosan microspheres could be useful for sustained release of tetanus toxoid to maintain the antibody titre for prolonged period.

Original languageEnglish
Pages (from-to)42-49
Number of pages8
JournalInternational Journal of Advances in Pharmaceutical Sciences
Volume1
Issue number1
DOIs
Publication statusPublished - 01-12-2010

Fingerprint

vaccines
Tetanus Toxoid
Chitosan
calcium oxides
Microspheres
delivery
Vaccines
guinea pigs
formulations
dosage
chromatography
antibodies
emulsions
affinity
toluene
optics
Citrus aurantiifolia
adsorption
polymers
Flocculation

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science
  • Physical and Theoretical Chemistry

Cite this

@article{07f493240d004ee2bd674e7355bcd720,
title = "Single dose vaccine delivery system of tetanus toxoid formulation based on chitosan microspheres",
abstract = "Background: Biodegradable polymers such as Chitosan or PLGA has been used for sustain release of vaccines for prolonged period. Purpose of the study: To develop single dose vaccine delivery system of tetanus toxoid using chitosan microspheres. Different batches, 1{\%}, 2{\%} and 3{\%} of chitosan microspheres (CM) were prepared by emulsion cross-linking method using glutaradehyde saturated toluene (GST). The tetanus toxoid (TT) was loaded with 1{\%}, 2{\%} and 3{\%} of CM by adsorption method. Percentage of TT absorbed in 1{\%}, 2{\%} and 3{\%} CM were determined by Limes flocculation method. In vitro release of adsorbed TT from 1{\%}, 2{\%} and 3{\%} CM were performed with PBS (pH 7.4) and evaluated by ELISA assay using purified guinea pig IgG by affinity chromatography. Results: The average particle size of 1{\%}, 2{\%} and 3{\%} of CM were determined by optical microscopic method and particle size was found to be 30μm, 31μm, and 35μm respectively. Percentage of TT adsorbed in 1{\%}, 2{\%} and 3{\%} CM were found to be 71{\%}, 80{\%} and 76{\%} respectively by Limes flocculation method. In vitro studies showed that different batches (1{\%}, 2{\%} and 3{\%}) of CM showed cumulative percentage release of TT 74.09{\%}, 89.31{\%}, and 80.23{\%} respectively for 50 days. Among three batches of microspheres, 2{\%} of chitosan microspheres showed better consistent and sustained release of TT for 50 days. Conclusions: This study suggested that 2{\%} of chitosan microspheres could be useful for sustained release of tetanus toxoid to maintain the antibody titre for prolonged period.",
author = "B. Dineshkumar and Dhanaraj, {S. A.} and K. Santhi and P. Vijayan and Raghu Chandrasekhar",
year = "2010",
month = "12",
day = "1",
doi = "10.5138/ijaps.2010.0976.1055.01004",
language = "English",
volume = "1",
pages = "42--49",
journal = "International Journal of Advances in Pharmaceutical Sciences",
issn = "0976-1055",
publisher = "Advanced Research Journals",
number = "1",

}

Single dose vaccine delivery system of tetanus toxoid formulation based on chitosan microspheres. / Dineshkumar, B.; Dhanaraj, S. A.; Santhi, K.; Vijayan, P.; Chandrasekhar, Raghu.

In: International Journal of Advances in Pharmaceutical Sciences, Vol. 1, No. 1, 01.12.2010, p. 42-49.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Single dose vaccine delivery system of tetanus toxoid formulation based on chitosan microspheres

AU - Dineshkumar, B.

AU - Dhanaraj, S. A.

AU - Santhi, K.

AU - Vijayan, P.

AU - Chandrasekhar, Raghu

PY - 2010/12/1

Y1 - 2010/12/1

N2 - Background: Biodegradable polymers such as Chitosan or PLGA has been used for sustain release of vaccines for prolonged period. Purpose of the study: To develop single dose vaccine delivery system of tetanus toxoid using chitosan microspheres. Different batches, 1%, 2% and 3% of chitosan microspheres (CM) were prepared by emulsion cross-linking method using glutaradehyde saturated toluene (GST). The tetanus toxoid (TT) was loaded with 1%, 2% and 3% of CM by adsorption method. Percentage of TT absorbed in 1%, 2% and 3% CM were determined by Limes flocculation method. In vitro release of adsorbed TT from 1%, 2% and 3% CM were performed with PBS (pH 7.4) and evaluated by ELISA assay using purified guinea pig IgG by affinity chromatography. Results: The average particle size of 1%, 2% and 3% of CM were determined by optical microscopic method and particle size was found to be 30μm, 31μm, and 35μm respectively. Percentage of TT adsorbed in 1%, 2% and 3% CM were found to be 71%, 80% and 76% respectively by Limes flocculation method. In vitro studies showed that different batches (1%, 2% and 3%) of CM showed cumulative percentage release of TT 74.09%, 89.31%, and 80.23% respectively for 50 days. Among three batches of microspheres, 2% of chitosan microspheres showed better consistent and sustained release of TT for 50 days. Conclusions: This study suggested that 2% of chitosan microspheres could be useful for sustained release of tetanus toxoid to maintain the antibody titre for prolonged period.

AB - Background: Biodegradable polymers such as Chitosan or PLGA has been used for sustain release of vaccines for prolonged period. Purpose of the study: To develop single dose vaccine delivery system of tetanus toxoid using chitosan microspheres. Different batches, 1%, 2% and 3% of chitosan microspheres (CM) were prepared by emulsion cross-linking method using glutaradehyde saturated toluene (GST). The tetanus toxoid (TT) was loaded with 1%, 2% and 3% of CM by adsorption method. Percentage of TT absorbed in 1%, 2% and 3% CM were determined by Limes flocculation method. In vitro release of adsorbed TT from 1%, 2% and 3% CM were performed with PBS (pH 7.4) and evaluated by ELISA assay using purified guinea pig IgG by affinity chromatography. Results: The average particle size of 1%, 2% and 3% of CM were determined by optical microscopic method and particle size was found to be 30μm, 31μm, and 35μm respectively. Percentage of TT adsorbed in 1%, 2% and 3% CM were found to be 71%, 80% and 76% respectively by Limes flocculation method. In vitro studies showed that different batches (1%, 2% and 3%) of CM showed cumulative percentage release of TT 74.09%, 89.31%, and 80.23% respectively for 50 days. Among three batches of microspheres, 2% of chitosan microspheres showed better consistent and sustained release of TT for 50 days. Conclusions: This study suggested that 2% of chitosan microspheres could be useful for sustained release of tetanus toxoid to maintain the antibody titre for prolonged period.

UR - http://www.scopus.com/inward/record.url?scp=79952024330&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952024330&partnerID=8YFLogxK

U2 - 10.5138/ijaps.2010.0976.1055.01004

DO - 10.5138/ijaps.2010.0976.1055.01004

M3 - Article

VL - 1

SP - 42

EP - 49

JO - International Journal of Advances in Pharmaceutical Sciences

JF - International Journal of Advances in Pharmaceutical Sciences

SN - 0976-1055

IS - 1

ER -