Single nucleotide polymorphisms of ADRB2 gene and their association with susceptibility for Plasmodium falciparum malaria and asthma in an Indian population

Abdul Vahab Saadi, Himanshu Gupta, Arshia Angural, Sreeja Kumari Dhanya, Sridevi Mony, Devesh Oberoi, Sydney C. D'Souza, Ramesh Chandra Sahoo, Manjunath H. Hande, Puthiya Mundyat Gopinath, Kapaettu Satyamoorthy

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Abstract

The essential route to blood parasitaemia in malaria, erythrocyte invasion is facilitated by activation of the G-protein coupled receptor signaling pathway mediated by the β2-adrenoreceptor as one of the proteins on the surface of red blood cells. The effectiveness of bronchodilators and inhaled corticosteroids in the clinical treatment for asthma patients also depend on polymorphisms in the β2-adrenoreceptor gene (ADRB2). In a case control study, individuals affected by Plasmodium falciparum malaria, asthma and controls were tested for association of six ADRB2 single nucleotide polymorphisms (SNPs) viz. rs1042711, rs1801704, rs1042713, rs1042714, rs1042717 and rs1042718, by direct DNA sequencing. The rs1801704 locus was significantly associated with malaria when compared against controls. The rs1042713 polymorphism was associated with forced expiratory flow between 25% and 75% of the FVC in asthma patients, pre (p= 0.048) and post (p= 0.038) treatment measurements. Predicted haplotype of the six SNPs computed from genotype data showed T-T-A-C-G-C conferred significant risk of malaria (p= 0.02) whereas T-T-A-C-G-A was associated with risk of asthma (p= 0.02). The haplotype T-T-G-C-G-C was protective against both malaria (p= 0.02) as well as asthma (p= 0.026) and C-C-G-G-G-C was protective uniquely for asthma (p= 0.04). A significant outcome was that all variant alleles at the SNP loci were part of the haplotype conferring resistance to malaria disease and asthma, except rs1042713 and rs1042718 which showed very high frequency in asthma. The pairwise linkage disequilibrium (LD) estimates showed a distinct LD block of all SNP loci (D' = 1 or 0.8) in malaria patients. This characteristic haplotype block was disrupted in the controls due to non-significant pairwise LD of the SNP loci; and a more extensive disruption of the block was noted in asthma patients. The study provides evidence for the proposed role of β2-adrenoreceptor mediated molecular mechanisms in etiology of malaria, as well as asthma disease and drug response, for further clinical and therapeutic application studies.

Original languageEnglish
Pages (from-to)140-147
Number of pages8
JournalInfection, Genetics and Evolution
Volume20
DOIs
Publication statusPublished - 2013

Fingerprint

Falciparum Malaria
asthma
malaria
Plasmodium falciparum
single nucleotide polymorphism
Single Nucleotide Polymorphism
polymorphism
Asthma
Malaria
gene
Population
Genes
genes
Haplotypes
Linkage Disequilibrium
haplotypes
linkage disequilibrium
disequilibrium
loci
erythrocytes

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Microbiology (medical)
  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Molecular Biology
  • Microbiology

Cite this

Saadi, Abdul Vahab ; Gupta, Himanshu ; Angural, Arshia ; Dhanya, Sreeja Kumari ; Mony, Sridevi ; Oberoi, Devesh ; D'Souza, Sydney C. ; Sahoo, Ramesh Chandra ; Hande, Manjunath H. ; Gopinath, Puthiya Mundyat ; Satyamoorthy, Kapaettu. / Single nucleotide polymorphisms of ADRB2 gene and their association with susceptibility for Plasmodium falciparum malaria and asthma in an Indian population. In: Infection, Genetics and Evolution. 2013 ; Vol. 20. pp. 140-147.
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abstract = "The essential route to blood parasitaemia in malaria, erythrocyte invasion is facilitated by activation of the G-protein coupled receptor signaling pathway mediated by the β2-adrenoreceptor as one of the proteins on the surface of red blood cells. The effectiveness of bronchodilators and inhaled corticosteroids in the clinical treatment for asthma patients also depend on polymorphisms in the β2-adrenoreceptor gene (ADRB2). In a case control study, individuals affected by Plasmodium falciparum malaria, asthma and controls were tested for association of six ADRB2 single nucleotide polymorphisms (SNPs) viz. rs1042711, rs1801704, rs1042713, rs1042714, rs1042717 and rs1042718, by direct DNA sequencing. The rs1801704 locus was significantly associated with malaria when compared against controls. The rs1042713 polymorphism was associated with forced expiratory flow between 25{\%} and 75{\%} of the FVC in asthma patients, pre (p= 0.048) and post (p= 0.038) treatment measurements. Predicted haplotype of the six SNPs computed from genotype data showed T-T-A-C-G-C conferred significant risk of malaria (p= 0.02) whereas T-T-A-C-G-A was associated with risk of asthma (p= 0.02). The haplotype T-T-G-C-G-C was protective against both malaria (p= 0.02) as well as asthma (p= 0.026) and C-C-G-G-G-C was protective uniquely for asthma (p= 0.04). A significant outcome was that all variant alleles at the SNP loci were part of the haplotype conferring resistance to malaria disease and asthma, except rs1042713 and rs1042718 which showed very high frequency in asthma. The pairwise linkage disequilibrium (LD) estimates showed a distinct LD block of all SNP loci (D' = 1 or 0.8) in malaria patients. This characteristic haplotype block was disrupted in the controls due to non-significant pairwise LD of the SNP loci; and a more extensive disruption of the block was noted in asthma patients. The study provides evidence for the proposed role of β2-adrenoreceptor mediated molecular mechanisms in etiology of malaria, as well as asthma disease and drug response, for further clinical and therapeutic application studies.",
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Single nucleotide polymorphisms of ADRB2 gene and their association with susceptibility for Plasmodium falciparum malaria and asthma in an Indian population. / Saadi, Abdul Vahab; Gupta, Himanshu; Angural, Arshia; Dhanya, Sreeja Kumari; Mony, Sridevi; Oberoi, Devesh; D'Souza, Sydney C.; Sahoo, Ramesh Chandra; Hande, Manjunath H.; Gopinath, Puthiya Mundyat; Satyamoorthy, Kapaettu.

In: Infection, Genetics and Evolution, Vol. 20, 2013, p. 140-147.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Single nucleotide polymorphisms of ADRB2 gene and their association with susceptibility for Plasmodium falciparum malaria and asthma in an Indian population

AU - Saadi, Abdul Vahab

AU - Gupta, Himanshu

AU - Angural, Arshia

AU - Dhanya, Sreeja Kumari

AU - Mony, Sridevi

AU - Oberoi, Devesh

AU - D'Souza, Sydney C.

AU - Sahoo, Ramesh Chandra

AU - Hande, Manjunath H.

AU - Gopinath, Puthiya Mundyat

AU - Satyamoorthy, Kapaettu

PY - 2013

Y1 - 2013

N2 - The essential route to blood parasitaemia in malaria, erythrocyte invasion is facilitated by activation of the G-protein coupled receptor signaling pathway mediated by the β2-adrenoreceptor as one of the proteins on the surface of red blood cells. The effectiveness of bronchodilators and inhaled corticosteroids in the clinical treatment for asthma patients also depend on polymorphisms in the β2-adrenoreceptor gene (ADRB2). In a case control study, individuals affected by Plasmodium falciparum malaria, asthma and controls were tested for association of six ADRB2 single nucleotide polymorphisms (SNPs) viz. rs1042711, rs1801704, rs1042713, rs1042714, rs1042717 and rs1042718, by direct DNA sequencing. The rs1801704 locus was significantly associated with malaria when compared against controls. The rs1042713 polymorphism was associated with forced expiratory flow between 25% and 75% of the FVC in asthma patients, pre (p= 0.048) and post (p= 0.038) treatment measurements. Predicted haplotype of the six SNPs computed from genotype data showed T-T-A-C-G-C conferred significant risk of malaria (p= 0.02) whereas T-T-A-C-G-A was associated with risk of asthma (p= 0.02). The haplotype T-T-G-C-G-C was protective against both malaria (p= 0.02) as well as asthma (p= 0.026) and C-C-G-G-G-C was protective uniquely for asthma (p= 0.04). A significant outcome was that all variant alleles at the SNP loci were part of the haplotype conferring resistance to malaria disease and asthma, except rs1042713 and rs1042718 which showed very high frequency in asthma. The pairwise linkage disequilibrium (LD) estimates showed a distinct LD block of all SNP loci (D' = 1 or 0.8) in malaria patients. This characteristic haplotype block was disrupted in the controls due to non-significant pairwise LD of the SNP loci; and a more extensive disruption of the block was noted in asthma patients. The study provides evidence for the proposed role of β2-adrenoreceptor mediated molecular mechanisms in etiology of malaria, as well as asthma disease and drug response, for further clinical and therapeutic application studies.

AB - The essential route to blood parasitaemia in malaria, erythrocyte invasion is facilitated by activation of the G-protein coupled receptor signaling pathway mediated by the β2-adrenoreceptor as one of the proteins on the surface of red blood cells. The effectiveness of bronchodilators and inhaled corticosteroids in the clinical treatment for asthma patients also depend on polymorphisms in the β2-adrenoreceptor gene (ADRB2). In a case control study, individuals affected by Plasmodium falciparum malaria, asthma and controls were tested for association of six ADRB2 single nucleotide polymorphisms (SNPs) viz. rs1042711, rs1801704, rs1042713, rs1042714, rs1042717 and rs1042718, by direct DNA sequencing. The rs1801704 locus was significantly associated with malaria when compared against controls. The rs1042713 polymorphism was associated with forced expiratory flow between 25% and 75% of the FVC in asthma patients, pre (p= 0.048) and post (p= 0.038) treatment measurements. Predicted haplotype of the six SNPs computed from genotype data showed T-T-A-C-G-C conferred significant risk of malaria (p= 0.02) whereas T-T-A-C-G-A was associated with risk of asthma (p= 0.02). The haplotype T-T-G-C-G-C was protective against both malaria (p= 0.02) as well as asthma (p= 0.026) and C-C-G-G-G-C was protective uniquely for asthma (p= 0.04). A significant outcome was that all variant alleles at the SNP loci were part of the haplotype conferring resistance to malaria disease and asthma, except rs1042713 and rs1042718 which showed very high frequency in asthma. The pairwise linkage disequilibrium (LD) estimates showed a distinct LD block of all SNP loci (D' = 1 or 0.8) in malaria patients. This characteristic haplotype block was disrupted in the controls due to non-significant pairwise LD of the SNP loci; and a more extensive disruption of the block was noted in asthma patients. The study provides evidence for the proposed role of β2-adrenoreceptor mediated molecular mechanisms in etiology of malaria, as well as asthma disease and drug response, for further clinical and therapeutic application studies.

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