TY - JOUR
T1 - Slime production a virulence marker in Pseudomonas aeruginosa strains isolated from clinical and environmental specimens
T2 - A comparative study of two methods
AU - Prasad, S. Vishnu
AU - Ballal, Mamatha
AU - Shivananda, P. G.
PY - 2009/4/1
Y1 - 2009/4/1
N2 - Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism. Here, comparative studies of two phenotypic methods using the tube method and the spectrophotometric method for slime production from 100 clinically and 21 environmentally significant isolates of P. aeruginosa were performed. A total of 68 isolates were positive by either of the tests whereas only 34 were positive by both the tests. The tube method detected slime significantly in more number of isolates than the spectrophotometric method. The tube test was found to be superior to the spectrophotometric method in ease of performance, interpretation and sensitivity. Among the clinical isolates, systemic isolates produce less slime compared to wound, respiratory and urinary isolates. Isolates from the hospital environment produced more slime indicating that this virulence marker helps the organism to survive for longer periods and cause nosocomial infections.
AB - Detection of slime in Pseudomonas aeruginosa can be useful in understanding the virulence of this organism. Here, comparative studies of two phenotypic methods using the tube method and the spectrophotometric method for slime production from 100 clinically and 21 environmentally significant isolates of P. aeruginosa were performed. A total of 68 isolates were positive by either of the tests whereas only 34 were positive by both the tests. The tube method detected slime significantly in more number of isolates than the spectrophotometric method. The tube test was found to be superior to the spectrophotometric method in ease of performance, interpretation and sensitivity. Among the clinical isolates, systemic isolates produce less slime compared to wound, respiratory and urinary isolates. Isolates from the hospital environment produced more slime indicating that this virulence marker helps the organism to survive for longer periods and cause nosocomial infections.
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U2 - 10.4103/0377-4929.48914
DO - 10.4103/0377-4929.48914
M3 - Article
C2 - 19332909
AN - SCOPUS:63449107411
SN - 0377-4929
VL - 52
SP - 191
EP - 193
JO - Indian Journal of Pathology and Microbiology
JF - Indian Journal of Pathology and Microbiology
IS - 2
ER -