Speciation and antibiogram of coagulase negative staphylococci (CONS) from various clinical specimens

D. S. Shubha, Sageera Banoo, V. Shashidhar, Farheen Fatima, D. Venkatesha

Research output: Contribution to journalArticle

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Abstract

Background: CONS are the major nosocomial pathogens. These undoubtedly need more recognition. Susceptibility testing should be done on any isolate considered to be a pathogen as multidrug resistance is common among them. Aims: • Isolation, identification and speciation of CONS. • To identify their pathogenicity markers, antibiogram with special emphasis on methicillin resistance. Settings and Design: This cross sectional study was conducted at a government tertiary healthcare teaching center for a period of one year. Material and Methods: Totally 2361 clinical specimens were processed. All specimens were subjected to gram's staining and cultured for isolation. The organisms were identified and speciated by standard biochemical reactions. Antibiogram was formed by Kirby-Bauer disk diffusion method. Statistical Analysis: The data entry was carried out using Microsoft office Excel worksheet and was statistically analyzed by WINKS SDA6 software. Results: A total of 150 strains of CONS were isolated from 2361 clinical specimens. Majority of CONS were isolated from urine (44.67%), followed by exudates (33.33%), and blood (22%). Most common species isolated was S.epidermidis (50.67%), followed by S.saprophyticus (22%) and S.hemolyticus (18%). Slime production (38.67%) and methicillin resistance (54.67%) was detected respectively. Vancomycin and amikacin were the most effective antibiotics. Conclusion: S.epidermidis can cause a number of human infections and should no longer be considered as a harmless commensal. S.saprophyticus is proven pathogen in man. The antibiotic resistance pattern in CONS is a great threat to clinicians combating such infections.

Original languageEnglish
Pages (from-to)91-95
Number of pages5
JournalIndian Journal of Public Health Research and Development
Volume3
Issue number1
Publication statusPublished - 01-01-2012

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Coagulase
Microbial Sensitivity Tests
Staphylococcus
Methicillin Resistance
Disk Diffusion Antimicrobial Tests
Statistical Data Interpretation
Amikacin
Multiple Drug Resistance
Exudates and Transudates
Tertiary Healthcare
Vancomycin
Microbial Drug Resistance
Infection
Virulence
Teaching
Software
Cross-Sectional Studies
Urine
Staining and Labeling
Anti-Bacterial Agents

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health

Cite this

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abstract = "Background: CONS are the major nosocomial pathogens. These undoubtedly need more recognition. Susceptibility testing should be done on any isolate considered to be a pathogen as multidrug resistance is common among them. Aims: • Isolation, identification and speciation of CONS. • To identify their pathogenicity markers, antibiogram with special emphasis on methicillin resistance. Settings and Design: This cross sectional study was conducted at a government tertiary healthcare teaching center for a period of one year. Material and Methods: Totally 2361 clinical specimens were processed. All specimens were subjected to gram's staining and cultured for isolation. The organisms were identified and speciated by standard biochemical reactions. Antibiogram was formed by Kirby-Bauer disk diffusion method. Statistical Analysis: The data entry was carried out using Microsoft office Excel worksheet and was statistically analyzed by WINKS SDA6 software. Results: A total of 150 strains of CONS were isolated from 2361 clinical specimens. Majority of CONS were isolated from urine (44.67{\%}), followed by exudates (33.33{\%}), and blood (22{\%}). Most common species isolated was S.epidermidis (50.67{\%}), followed by S.saprophyticus (22{\%}) and S.hemolyticus (18{\%}). Slime production (38.67{\%}) and methicillin resistance (54.67{\%}) was detected respectively. Vancomycin and amikacin were the most effective antibiotics. Conclusion: S.epidermidis can cause a number of human infections and should no longer be considered as a harmless commensal. S.saprophyticus is proven pathogen in man. The antibiotic resistance pattern in CONS is a great threat to clinicians combating such infections.",
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Speciation and antibiogram of coagulase negative staphylococci (CONS) from various clinical specimens. / Shubha, D. S.; Banoo, Sageera; Shashidhar, V.; Fatima, Farheen; Venkatesha, D.

In: Indian Journal of Public Health Research and Development, Vol. 3, No. 1, 01.01.2012, p. 91-95.

Research output: Contribution to journalArticle

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AU - Shubha, D. S.

AU - Banoo, Sageera

AU - Shashidhar, V.

AU - Fatima, Farheen

AU - Venkatesha, D.

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N2 - Background: CONS are the major nosocomial pathogens. These undoubtedly need more recognition. Susceptibility testing should be done on any isolate considered to be a pathogen as multidrug resistance is common among them. Aims: • Isolation, identification and speciation of CONS. • To identify their pathogenicity markers, antibiogram with special emphasis on methicillin resistance. Settings and Design: This cross sectional study was conducted at a government tertiary healthcare teaching center for a period of one year. Material and Methods: Totally 2361 clinical specimens were processed. All specimens were subjected to gram's staining and cultured for isolation. The organisms were identified and speciated by standard biochemical reactions. Antibiogram was formed by Kirby-Bauer disk diffusion method. Statistical Analysis: The data entry was carried out using Microsoft office Excel worksheet and was statistically analyzed by WINKS SDA6 software. Results: A total of 150 strains of CONS were isolated from 2361 clinical specimens. Majority of CONS were isolated from urine (44.67%), followed by exudates (33.33%), and blood (22%). Most common species isolated was S.epidermidis (50.67%), followed by S.saprophyticus (22%) and S.hemolyticus (18%). Slime production (38.67%) and methicillin resistance (54.67%) was detected respectively. Vancomycin and amikacin were the most effective antibiotics. Conclusion: S.epidermidis can cause a number of human infections and should no longer be considered as a harmless commensal. S.saprophyticus is proven pathogen in man. The antibiotic resistance pattern in CONS is a great threat to clinicians combating such infections.

AB - Background: CONS are the major nosocomial pathogens. These undoubtedly need more recognition. Susceptibility testing should be done on any isolate considered to be a pathogen as multidrug resistance is common among them. Aims: • Isolation, identification and speciation of CONS. • To identify their pathogenicity markers, antibiogram with special emphasis on methicillin resistance. Settings and Design: This cross sectional study was conducted at a government tertiary healthcare teaching center for a period of one year. Material and Methods: Totally 2361 clinical specimens were processed. All specimens were subjected to gram's staining and cultured for isolation. The organisms were identified and speciated by standard biochemical reactions. Antibiogram was formed by Kirby-Bauer disk diffusion method. Statistical Analysis: The data entry was carried out using Microsoft office Excel worksheet and was statistically analyzed by WINKS SDA6 software. Results: A total of 150 strains of CONS were isolated from 2361 clinical specimens. Majority of CONS were isolated from urine (44.67%), followed by exudates (33.33%), and blood (22%). Most common species isolated was S.epidermidis (50.67%), followed by S.saprophyticus (22%) and S.hemolyticus (18%). Slime production (38.67%) and methicillin resistance (54.67%) was detected respectively. Vancomycin and amikacin were the most effective antibiotics. Conclusion: S.epidermidis can cause a number of human infections and should no longer be considered as a harmless commensal. S.saprophyticus is proven pathogen in man. The antibiotic resistance pattern in CONS is a great threat to clinicians combating such infections.

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