Spectrofluorimetric method for determination of duloxetine hydrochloride in bulk and pharmaceutical dosage forms

S. Prabhu, S. Shahnawaz, C. Kumar, A. Shirwaikar

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of duloxetine hydrochloride in pure and pharmaceutical dosage form. Duloxetine hydrochloride showed strong native fluorescence in 0.05 M acetic acid having excitation at 225 nm and emission at 340 nm. Effect of different solvents were thoroughly investigated. The calibration graph was linear in the range from 0.020 to 0.400 μg/ml. The proposed method was statistically validated and successfully applied for analysis of capsule dosage forms. The limit of detection and limit of quantification were found to be 0.003 μg/ml and 0.010 μg/ml, respectively. The percentage recovery was found to be in the range of 98.71% to 99.17%.
Original languageEnglish
Pages (from-to)502-503
Number of pages2
JournalIndian Journal of Pharmaceutical Sciences
Volume70
Issue number4
DOIs
Publication statusPublished - 2008

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Dosage Forms
Acetic Acid
Pharmaceutical Preparations
Calibration
Capsules
Limit of Detection
Fluorescence
Duloxetine Hydrochloride

Cite this

@article{57a534af93a64fd7962b93a308fbf90c,
title = "Spectrofluorimetric method for determination of duloxetine hydrochloride in bulk and pharmaceutical dosage forms",
abstract = "A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of duloxetine hydrochloride in pure and pharmaceutical dosage form. Duloxetine hydrochloride showed strong native fluorescence in 0.05 M acetic acid having excitation at 225 nm and emission at 340 nm. Effect of different solvents were thoroughly investigated. The calibration graph was linear in the range from 0.020 to 0.400 μg/ml. The proposed method was statistically validated and successfully applied for analysis of capsule dosage forms. The limit of detection and limit of quantification were found to be 0.003 μg/ml and 0.010 μg/ml, respectively. The percentage recovery was found to be in the range of 98.71{\%} to 99.17{\%}.",
author = "S. Prabhu and S. Shahnawaz and C. Kumar and A. Shirwaikar",
note = "Cited By :14 Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Prabhu, S.; Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal-576 104, India; email: slaxmanvel@yahoo.com Chemicals/CAS: acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; duloxetine, 116539-59-4, 136434-34-9 Tradenames: dulojoy, Torrent, India; dumore, Lupin Laboratories, India Manufacturers: Lupin Laboratories, India; Torrent, India References: Sweetman, S.C., (2005) Martindale, The complete drug reference, p. 291. , 34th ed. London: Pharmaceutical Press;; Brunton, L.L., Parker, K.S., Lazo, J.S., (2005) Goodman and Gillman.s, The Pharmacological Basis of Therapeutics, pp. 436-450. , 11th ed. London: McGraw Hill Publishing;; Soni, P., Mariappan, T.T., Banerjee, U.C., High performance liquid chromatographic method for the simultaneous estimation of the key intermediates of Duloxetine (2005) Talanta, 67, pp. 975-978; Jansen, P.J., Oren, P.L., Kemp, C.A., Maple, S.R., Baertschi, S.W., Characterization of impurities formed by interaction of Duloxetine HCl with enteric polymers hydroxypropyl methylcellulose acetate succinate and hydroxypropyl methylcellulose phthalate (1998) J Pharm Sci, 87, pp. 81-85; Johnson, J.T., Oldham, S.W., Lantz, R.J., DeLong, A.F., High performance liquid chromatographic method for the determination of Duloxetine and desmethyl duloxetine in human plasma (1996) J Liq Chromatogr Rel Technol, 19, pp. 1631-1641",
year = "2008",
doi = "10.4103/0250-474X.44603",
language = "English",
volume = "70",
pages = "502--503",
journal = "Indian Journal of Pharmaceutical Sciences",
issn = "0250-474X",
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Spectrofluorimetric method for determination of duloxetine hydrochloride in bulk and pharmaceutical dosage forms. / Prabhu, S.; Shahnawaz, S.; Kumar, C.; Shirwaikar, A.

In: Indian Journal of Pharmaceutical Sciences, Vol. 70, No. 4, 2008, p. 502-503.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Spectrofluorimetric method for determination of duloxetine hydrochloride in bulk and pharmaceutical dosage forms

AU - Prabhu, S.

AU - Shahnawaz, S.

AU - Kumar, C.

AU - Shirwaikar, A.

N1 - Cited By :14 Export Date: 10 November 2017 CODEN: IJSID Correspondence Address: Prabhu, S.; Department of Pharmaceutical Quality Assurance, Manipal College of Pharmaceutical Sciences, Manipal-576 104, India; email: slaxmanvel@yahoo.com Chemicals/CAS: acetic acid, 127-08-2, 127-09-3, 64-19-7, 71-50-1; duloxetine, 116539-59-4, 136434-34-9 Tradenames: dulojoy, Torrent, India; dumore, Lupin Laboratories, India Manufacturers: Lupin Laboratories, India; Torrent, India References: Sweetman, S.C., (2005) Martindale, The complete drug reference, p. 291. , 34th ed. London: Pharmaceutical Press;; Brunton, L.L., Parker, K.S., Lazo, J.S., (2005) Goodman and Gillman.s, The Pharmacological Basis of Therapeutics, pp. 436-450. , 11th ed. London: McGraw Hill Publishing;; Soni, P., Mariappan, T.T., Banerjee, U.C., High performance liquid chromatographic method for the simultaneous estimation of the key intermediates of Duloxetine (2005) Talanta, 67, pp. 975-978; Jansen, P.J., Oren, P.L., Kemp, C.A., Maple, S.R., Baertschi, S.W., Characterization of impurities formed by interaction of Duloxetine HCl with enteric polymers hydroxypropyl methylcellulose acetate succinate and hydroxypropyl methylcellulose phthalate (1998) J Pharm Sci, 87, pp. 81-85; Johnson, J.T., Oldham, S.W., Lantz, R.J., DeLong, A.F., High performance liquid chromatographic method for the determination of Duloxetine and desmethyl duloxetine in human plasma (1996) J Liq Chromatogr Rel Technol, 19, pp. 1631-1641

PY - 2008

Y1 - 2008

N2 - A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of duloxetine hydrochloride in pure and pharmaceutical dosage form. Duloxetine hydrochloride showed strong native fluorescence in 0.05 M acetic acid having excitation at 225 nm and emission at 340 nm. Effect of different solvents were thoroughly investigated. The calibration graph was linear in the range from 0.020 to 0.400 μg/ml. The proposed method was statistically validated and successfully applied for analysis of capsule dosage forms. The limit of detection and limit of quantification were found to be 0.003 μg/ml and 0.010 μg/ml, respectively. The percentage recovery was found to be in the range of 98.71% to 99.17%.

AB - A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of duloxetine hydrochloride in pure and pharmaceutical dosage form. Duloxetine hydrochloride showed strong native fluorescence in 0.05 M acetic acid having excitation at 225 nm and emission at 340 nm. Effect of different solvents were thoroughly investigated. The calibration graph was linear in the range from 0.020 to 0.400 μg/ml. The proposed method was statistically validated and successfully applied for analysis of capsule dosage forms. The limit of detection and limit of quantification were found to be 0.003 μg/ml and 0.010 μg/ml, respectively. The percentage recovery was found to be in the range of 98.71% to 99.17%.

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DO - 10.4103/0250-474X.44603

M3 - Article

VL - 70

SP - 502

EP - 503

JO - Indian Journal of Pharmaceutical Sciences

JF - Indian Journal of Pharmaceutical Sciences

SN - 0250-474X

IS - 4

ER -