Spectrofluorimetric method for determination of etoposide in bulk and pharmaceutical dosage forms

S. Lakshmana Prabu, S. Shahnawaz, C. D. Kumar, S. G. Vasantharaju, N. Sivagurunathan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple, accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of etoposide in pure and pharmaceutical dosage forms. Etoposide showed strong native fluorescence in 0.05 M sulphuric acid having at 227.0 nm and emission at 335 nm. The calibration graph was linear in the range from 0.01mcg/mL to 0.100 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of dosage form. The limit of detection and limit of quantification was found to be 0.0023 mcg/mL and 0.007 mcg/mL, respectively. The percent recovery was found to be in the range of 99.56% and 99.70%.

Original languageEnglish
Pages (from-to)58-60
Number of pages3
JournalIndian Drugs
Volume46
Issue number12
Publication statusPublished - 01-12-2009

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Dosage Forms
Etoposide
Pharmaceutical Preparations
Calibration
Limit of Detection
Fluorescence
Acids

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Lakshmana Prabu, S., Shahnawaz, S., Kumar, C. D., Vasantharaju, S. G., & Sivagurunathan, N. (2009). Spectrofluorimetric method for determination of etoposide in bulk and pharmaceutical dosage forms. Indian Drugs, 46(12), 58-60.
Lakshmana Prabu, S. ; Shahnawaz, S. ; Kumar, C. D. ; Vasantharaju, S. G. ; Sivagurunathan, N. / Spectrofluorimetric method for determination of etoposide in bulk and pharmaceutical dosage forms. In: Indian Drugs. 2009 ; Vol. 46, No. 12. pp. 58-60.
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Lakshmana Prabu, S, Shahnawaz, S, Kumar, CD, Vasantharaju, SG & Sivagurunathan, N 2009, 'Spectrofluorimetric method for determination of etoposide in bulk and pharmaceutical dosage forms', Indian Drugs, vol. 46, no. 12, pp. 58-60.

Spectrofluorimetric method for determination of etoposide in bulk and pharmaceutical dosage forms. / Lakshmana Prabu, S.; Shahnawaz, S.; Kumar, C. D.; Vasantharaju, S. G.; Sivagurunathan, N.

In: Indian Drugs, Vol. 46, No. 12, 01.12.2009, p. 58-60.

Research output: Contribution to journalArticle

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AU - Lakshmana Prabu, S.

AU - Shahnawaz, S.

AU - Kumar, C. D.

AU - Vasantharaju, S. G.

AU - Sivagurunathan, N.

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AB - A simple, accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of etoposide in pure and pharmaceutical dosage forms. Etoposide showed strong native fluorescence in 0.05 M sulphuric acid having at 227.0 nm and emission at 335 nm. The calibration graph was linear in the range from 0.01mcg/mL to 0.100 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of dosage form. The limit of detection and limit of quantification was found to be 0.0023 mcg/mL and 0.007 mcg/mL, respectively. The percent recovery was found to be in the range of 99.56% and 99.70%.

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Lakshmana Prabu S, Shahnawaz S, Kumar CD, Vasantharaju SG, Sivagurunathan N. Spectrofluorimetric method for determination of etoposide in bulk and pharmaceutical dosage forms. Indian Drugs. 2009 Dec 1;46(12):58-60.