Spectrofluorimetric method for determination of etoposide in bulk and pharmaceutical dosage forms

S. Lakshmana Prabu, S. Shahnawaz, C. D. Kumar, S. G. Vasantharaju, N. Sivagurunathan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple, accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of etoposide in pure and pharmaceutical dosage forms. Etoposide showed strong native fluorescence in 0.05 M sulphuric acid having at 227.0 nm and emission at 335 nm. The calibration graph was linear in the range from 0.01mcg/mL to 0.100 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of dosage form. The limit of detection and limit of quantification was found to be 0.0023 mcg/mL and 0.007 mcg/mL, respectively. The percent recovery was found to be in the range of 99.56% and 99.70%.

Original languageEnglish
Pages (from-to)58-60
Number of pages3
JournalIndian Drugs
Volume46
Issue number12
Publication statusPublished - 01-12-2009

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

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