Spectrofluorimetric method for determination of tramadol hydrochloride in bulk and pharmaceutical dosage forms

S. Lakshmana Prabu, S. Shahnawaz, C. Dinesh Kumar, S. G. Vasantharaju, J. Abraham

Research output: Contribution to journalArticle

Abstract

A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of Tramadol hydrochloride in pure and pharmaceutical dosage form. Tramadol hydrochloride showed strong native fluorescence in 0.025 M sulphuric acid having excitation at 220.0 nm and emission at 297±5 nm. All parameters viz; effect of different solvents, pH, dilutions, reaction time, temperature and effect of excipients were thoroughly investigated. The calibration graph was linear in the range from 0.025 to 0.400 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The limit of detection and limit of quantification were found to be 0.0053 mcg/mL and 0.0170 mcg/mL srespectively. The percentage recovery was found to be 99.42%.

Original languageEnglish
Pages (from-to)32-34
Number of pages3
JournalIndian Drugs
Volume46
Issue number8
Publication statusPublished - 01-08-2009

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

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