Spectrofluorimetric method for determination of tramadol hydrochloride in bulk and pharmaceutical dosage forms

S. Lakshmana Prabu, S. Shahnawaz, C. Dinesh Kumar, S. G. Vasantharaju, J. Abraham

Research output: Contribution to journalArticle

Abstract

A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of Tramadol hydrochloride in pure and pharmaceutical dosage form. Tramadol hydrochloride showed strong native fluorescence in 0.025 M sulphuric acid having excitation at 220.0 nm and emission at 297±5 nm. All parameters viz; effect of different solvents, pH, dilutions, reaction time, temperature and effect of excipients were thoroughly investigated. The calibration graph was linear in the range from 0.025 to 0.400 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The limit of detection and limit of quantification were found to be 0.0053 mcg/mL and 0.0170 mcg/mL srespectively. The percentage recovery was found to be 99.42%.

Original languageEnglish
Pages (from-to)32-34
Number of pages3
JournalIndian Drugs
Volume46
Issue number8
Publication statusPublished - 01-08-2009

Fingerprint

Tramadol
Dosage Forms
Excipients
Pharmaceutical Preparations
Calibration
Tablets
Limit of Detection
Fluorescence
Temperature
Acids

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery

Cite this

Lakshmana Prabu, S. ; Shahnawaz, S. ; Dinesh Kumar, C. ; Vasantharaju, S. G. ; Abraham, J. / Spectrofluorimetric method for determination of tramadol hydrochloride in bulk and pharmaceutical dosage forms. In: Indian Drugs. 2009 ; Vol. 46, No. 8. pp. 32-34.
@article{6c4a883b9f324c56898c92acf8cc0415,
title = "Spectrofluorimetric method for determination of tramadol hydrochloride in bulk and pharmaceutical dosage forms",
abstract = "A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of Tramadol hydrochloride in pure and pharmaceutical dosage form. Tramadol hydrochloride showed strong native fluorescence in 0.025 M sulphuric acid having excitation at 220.0 nm and emission at 297±5 nm. All parameters viz; effect of different solvents, pH, dilutions, reaction time, temperature and effect of excipients were thoroughly investigated. The calibration graph was linear in the range from 0.025 to 0.400 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The limit of detection and limit of quantification were found to be 0.0053 mcg/mL and 0.0170 mcg/mL srespectively. The percentage recovery was found to be 99.42{\%}.",
author = "{Lakshmana Prabu}, S. and S. Shahnawaz and {Dinesh Kumar}, C. and Vasantharaju, {S. G.} and J. Abraham",
year = "2009",
month = "8",
day = "1",
language = "English",
volume = "46",
pages = "32--34",
journal = "Indian Drugs",
issn = "0019-462X",
publisher = "Indian Drug Manufacturers' Association",
number = "8",

}

Lakshmana Prabu, S, Shahnawaz, S, Dinesh Kumar, C, Vasantharaju, SG & Abraham, J 2009, 'Spectrofluorimetric method for determination of tramadol hydrochloride in bulk and pharmaceutical dosage forms', Indian Drugs, vol. 46, no. 8, pp. 32-34.

Spectrofluorimetric method for determination of tramadol hydrochloride in bulk and pharmaceutical dosage forms. / Lakshmana Prabu, S.; Shahnawaz, S.; Dinesh Kumar, C.; Vasantharaju, S. G.; Abraham, J.

In: Indian Drugs, Vol. 46, No. 8, 01.08.2009, p. 32-34.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Spectrofluorimetric method for determination of tramadol hydrochloride in bulk and pharmaceutical dosage forms

AU - Lakshmana Prabu, S.

AU - Shahnawaz, S.

AU - Dinesh Kumar, C.

AU - Vasantharaju, S. G.

AU - Abraham, J.

PY - 2009/8/1

Y1 - 2009/8/1

N2 - A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of Tramadol hydrochloride in pure and pharmaceutical dosage form. Tramadol hydrochloride showed strong native fluorescence in 0.025 M sulphuric acid having excitation at 220.0 nm and emission at 297±5 nm. All parameters viz; effect of different solvents, pH, dilutions, reaction time, temperature and effect of excipients were thoroughly investigated. The calibration graph was linear in the range from 0.025 to 0.400 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The limit of detection and limit of quantification were found to be 0.0053 mcg/mL and 0.0170 mcg/mL srespectively. The percentage recovery was found to be 99.42%.

AB - A simple accurate, sensitive and reproducible spectrofluorimetric method was developed for the analysis of Tramadol hydrochloride in pure and pharmaceutical dosage form. Tramadol hydrochloride showed strong native fluorescence in 0.025 M sulphuric acid having excitation at 220.0 nm and emission at 297±5 nm. All parameters viz; effect of different solvents, pH, dilutions, reaction time, temperature and effect of excipients were thoroughly investigated. The calibration graph was linear in the range from 0.025 to 0.400 mcg/mL. The proposed method was statistically validated and successfully applied for analysis of tablet dosage forms. The limit of detection and limit of quantification were found to be 0.0053 mcg/mL and 0.0170 mcg/mL srespectively. The percentage recovery was found to be 99.42%.

UR - http://www.scopus.com/inward/record.url?scp=77955379955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955379955&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:77955379955

VL - 46

SP - 32

EP - 34

JO - Indian Drugs

JF - Indian Drugs

SN - 0019-462X

IS - 8

ER -