Spectrofluorimetric method for the determination of trifluoperazine hydrochloride in bulk and pharmaceutical dosage forms

S.G. Vasantharaju, S. Lakshmana Prabu, A. Jacob, P.C. Jagdish, N. Sivagurunathan

Research output: Contribution to journalArticlepeer-review

Abstract

A simple, precise and rapid fluorimetric method was developed for the determination of trifluoperazine hydrochloride in pharmaceutical dosage forms. Spectrofluorimetric method is based on the measurement of the native fluorescence and has always proved selective with relatively low detection limits. Trifluoperazine hydrochloride showed strong native fluorescence in water with excitation at 305.0 nm and emission at 480.0 nm. All parameters, viz; effect of different solvents and pH, were thoroughly investigated. The effect of potential interference due to common ingredients was investigated. The proposed method was statistically validated for linearity, accuracy and precision following International Conference on Harmonization, 1996 (ICH) recommendations and the USP (2003). Fluorescence intensity versus concentration in calibration graph was rectilinear in the range from 8 - 20 mcg/mL. The intra-day and inter-day precision were found to be 0.30 - 0.56, 0.43 - 0.71, respectively. The percent recovery was found to be 99.21±0.14. The method was successfully applied for the assay of trifluoperazine in pure form and in pharmaceutical dosage form.
Original languageEnglish
Pages (from-to)821-823
Number of pages3
JournalIndian Drugs
Volume45
Issue number10
Publication statusPublished - 2008

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