Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts

Fiona D’Souza, Shubhashree Uppangala, Gitanjali Asampille, Sujith Raj Salian, Guruprasad Kalthur, Riccardo Talevi, Hanudatta S. Atreya, Satish Kumar Adiga

Research output: Contribution to journalArticle

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Abstract

The metabolomic profile of an embryo culture medium can aid in the advanced prediction of embryonic developmental potential and genetic integrity. But it is not known if this technology can be used to determine the in vitro potential of inner cell mass (ICM) in adherence and proliferation. Here, we investigated the developmental potential of mouse 2-cell embryos carrying cisplatin-induced DNA lesions (IDL), beyond blastocyst stage using ICM outgrowth assay. The genetic integrity of ICM cells was determined by comet assay. The metabolic signatures of spent medium were recorded 84 hours post injection of hCG (hpi-hCG), and after 96 hours of extended in vitro culture (Ex 96) by NMR spectroscopy. We observed that blastocysts that lack the ability to adhere in vitro had an increased requirement of pyruvate (p < 0.01), lactate (p < 0.01), and were accompanied by a significant reduction of pyruvate-alanine ratio in the culture medium. We propose that the aforementioned metabolites from 84 hpi-hCG spent medium be further explored using appropriate experimental models, to prove their potential as biomarkers in the prediction of implantation ability of in vitro derived human embryos in clinical settings.

Original languageEnglish
Article number17025
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 01-12-2018

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Blastocyst
Culture Media
Embryonic Structures
Pyruvic Acid
Injections
Comet Assay
Metabolomics
Alanine
Cisplatin
Lactic Acid
Theoretical Models
Magnetic Resonance Spectroscopy
Biomarkers
In Vitro Techniques
Technology
DNA

All Science Journal Classification (ASJC) codes

  • General

Cite this

D’Souza, Fiona ; Uppangala, Shubhashree ; Asampille, Gitanjali ; Salian, Sujith Raj ; Kalthur, Guruprasad ; Talevi, Riccardo ; Atreya, Hanudatta S. ; Adiga, Satish Kumar. / Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts. In: Scientific Reports. 2018 ; Vol. 8, No. 1.
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Spent embryo culture medium metabolites are related to the in vitro attachment ability of blastocysts. / D’Souza, Fiona; Uppangala, Shubhashree; Asampille, Gitanjali; Salian, Sujith Raj; Kalthur, Guruprasad; Talevi, Riccardo; Atreya, Hanudatta S.; Adiga, Satish Kumar.

In: Scientific Reports, Vol. 8, No. 1, 17025, 01.12.2018.

Research output: Contribution to journalArticle

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