Sperm abnormalities induced by pre-pubertal exposure to cyclophosphamide are effectively mitigated by Moringa oleifera leaf extract

G. Nayak, A. Vadinkar, S. Nair, S. G. Kalthur, A. S. D'Souza, P. K. Shetty, S. Mutalik, M. M. Shetty, G. Kalthur, S. K. Adiga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Moringa oleifera L. is a medicinal plant with potential antioxidant property. This study was aimed at investigating the chemoprotective effect of Moringa oleifera leaf extract (MOE) on cyclophosphamide (CP)-induced testicular toxicity. Two-week-old male Swiss albino mice were intraperitoneally injected with phosphate-buffered saline, 50 mg kg-1 of CP and 25 mg kg-1 of MOE. In combination treatment, mice were injected with 25 mg kg-1 of MOE 24 h prior to CP injection, 24 h prior and post-CP injection and 24 h post-CP injection for 5 consecutive days (10 mg kg-1). Six weeks later, mice were sacrificed to assess epididymal sperm parameters. MOE alone did not have any significant effect on sperm parameters. However, acute injection of CP resulted in significant decline in motility (P < 0.001), increase in head abnormality (P < 0.01) and DNA damage (P < 0.05). Combining MOE with CP increased the sperm density, motility and reduced head defect and DNA damage, irrespective of the schedule and dosage of MOE. Administration of MOE prior to CP significantly elevated the level of superoxide dismutase and catalase with concomitant decrease in lipid peroxidation in the testicular tissue. In conclusion, MOE may have potential benefit in reducing the loss of male gonadal function following chemotherapy.

Original languageEnglish
Pages (from-to)125-136
Number of pages12
JournalAndrologia
Volume48
Issue number2
DOIs
Publication statusPublished - 01-03-2016

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Moringa oleifera
Cyclophosphamide
Spermatozoa
Injections
DNA Damage
Head
Sperm Motility
Medicinal Plants
Catalase
Lipid Peroxidation
Superoxide Dismutase
Appointments and Schedules
Antioxidants
Phosphates

All Science Journal Classification (ASJC) codes

  • Endocrinology
  • Urology

Cite this

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title = "Sperm abnormalities induced by pre-pubertal exposure to cyclophosphamide are effectively mitigated by Moringa oleifera leaf extract",
abstract = "Moringa oleifera L. is a medicinal plant with potential antioxidant property. This study was aimed at investigating the chemoprotective effect of Moringa oleifera leaf extract (MOE) on cyclophosphamide (CP)-induced testicular toxicity. Two-week-old male Swiss albino mice were intraperitoneally injected with phosphate-buffered saline, 50 mg kg-1 of CP and 25 mg kg-1 of MOE. In combination treatment, mice were injected with 25 mg kg-1 of MOE 24 h prior to CP injection, 24 h prior and post-CP injection and 24 h post-CP injection for 5 consecutive days (10 mg kg-1). Six weeks later, mice were sacrificed to assess epididymal sperm parameters. MOE alone did not have any significant effect on sperm parameters. However, acute injection of CP resulted in significant decline in motility (P < 0.001), increase in head abnormality (P < 0.01) and DNA damage (P < 0.05). Combining MOE with CP increased the sperm density, motility and reduced head defect and DNA damage, irrespective of the schedule and dosage of MOE. Administration of MOE prior to CP significantly elevated the level of superoxide dismutase and catalase with concomitant decrease in lipid peroxidation in the testicular tissue. In conclusion, MOE may have potential benefit in reducing the loss of male gonadal function following chemotherapy.",
author = "G. Nayak and A. Vadinkar and S. Nair and Kalthur, {S. G.} and D'Souza, {A. S.} and Shetty, {P. K.} and S. Mutalik and Shetty, {M. M.} and G. Kalthur and Adiga, {S. K.}",
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Sperm abnormalities induced by pre-pubertal exposure to cyclophosphamide are effectively mitigated by Moringa oleifera leaf extract. / Nayak, G.; Vadinkar, A.; Nair, S.; Kalthur, S. G.; D'Souza, A. S.; Shetty, P. K.; Mutalik, S.; Shetty, M. M.; Kalthur, G.; Adiga, S. K.

In: Andrologia, Vol. 48, No. 2, 01.03.2016, p. 125-136.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sperm abnormalities induced by pre-pubertal exposure to cyclophosphamide are effectively mitigated by Moringa oleifera leaf extract

AU - Nayak, G.

AU - Vadinkar, A.

AU - Nair, S.

AU - Kalthur, S. G.

AU - D'Souza, A. S.

AU - Shetty, P. K.

AU - Mutalik, S.

AU - Shetty, M. M.

AU - Kalthur, G.

AU - Adiga, S. K.

PY - 2016/3/1

Y1 - 2016/3/1

N2 - Moringa oleifera L. is a medicinal plant with potential antioxidant property. This study was aimed at investigating the chemoprotective effect of Moringa oleifera leaf extract (MOE) on cyclophosphamide (CP)-induced testicular toxicity. Two-week-old male Swiss albino mice were intraperitoneally injected with phosphate-buffered saline, 50 mg kg-1 of CP and 25 mg kg-1 of MOE. In combination treatment, mice were injected with 25 mg kg-1 of MOE 24 h prior to CP injection, 24 h prior and post-CP injection and 24 h post-CP injection for 5 consecutive days (10 mg kg-1). Six weeks later, mice were sacrificed to assess epididymal sperm parameters. MOE alone did not have any significant effect on sperm parameters. However, acute injection of CP resulted in significant decline in motility (P < 0.001), increase in head abnormality (P < 0.01) and DNA damage (P < 0.05). Combining MOE with CP increased the sperm density, motility and reduced head defect and DNA damage, irrespective of the schedule and dosage of MOE. Administration of MOE prior to CP significantly elevated the level of superoxide dismutase and catalase with concomitant decrease in lipid peroxidation in the testicular tissue. In conclusion, MOE may have potential benefit in reducing the loss of male gonadal function following chemotherapy.

AB - Moringa oleifera L. is a medicinal plant with potential antioxidant property. This study was aimed at investigating the chemoprotective effect of Moringa oleifera leaf extract (MOE) on cyclophosphamide (CP)-induced testicular toxicity. Two-week-old male Swiss albino mice were intraperitoneally injected with phosphate-buffered saline, 50 mg kg-1 of CP and 25 mg kg-1 of MOE. In combination treatment, mice were injected with 25 mg kg-1 of MOE 24 h prior to CP injection, 24 h prior and post-CP injection and 24 h post-CP injection for 5 consecutive days (10 mg kg-1). Six weeks later, mice were sacrificed to assess epididymal sperm parameters. MOE alone did not have any significant effect on sperm parameters. However, acute injection of CP resulted in significant decline in motility (P < 0.001), increase in head abnormality (P < 0.01) and DNA damage (P < 0.05). Combining MOE with CP increased the sperm density, motility and reduced head defect and DNA damage, irrespective of the schedule and dosage of MOE. Administration of MOE prior to CP significantly elevated the level of superoxide dismutase and catalase with concomitant decrease in lipid peroxidation in the testicular tissue. In conclusion, MOE may have potential benefit in reducing the loss of male gonadal function following chemotherapy.

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