Sperm DNA damage: Causes and laboratory detection

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Male factor infertility remains a significant problem affecting approximately one couple in six. The genetic integrity of a male gamete is necessary for the normal fertilization and development of the offspring. It has been shown that any insult to sperm DNA may have adverse influence on the reproductive outcome. Most human ejaculates contain a subpopulation of sperm with DNA damage, also referred to as DNA fragmentation, in the form of double- or single-strand breaks which have been induced in the DNA prior to or following ejaculation. The routine semen analysis does not reveal the genetic integrity of the sperm; hence, special techniques are employed for the detection of sperm DNA fragmentation. This chapter discusses about the causes of sperm DNA damage and various techniques employed to detect DNA fragmentation in spermatozoa.

Original languageEnglish
Title of host publicationMale Infertility
Subtitle of host publicationA Clinical Approach
PublisherSpringer India
Pages155-165
Number of pages11
ISBN (Electronic)9788132236047
ISBN (Print)9788132236023
DOIs
Publication statusPublished - 01-01-2016

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All Science Journal Classification (ASJC) codes

  • Health Professions(all)
  • Medicine(all)

Cite this

Adiga, S. K., & Kalthur, G. (2016). Sperm DNA damage: Causes and laboratory detection. In Male Infertility: A Clinical Approach (pp. 155-165). Springer India. https://doi.org/10.1007/978-81-322-3604-7_10