Sperm-mediated DNA lesions alter metabolite levels in spent embryo culture medium

Fiona D. Souza, Gitanjali Asampille, Shubhashree Uppangala, Guruprasad Kalthur, Hanudatta S. Atreya, Satish Kumar Adiga

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Paternal genetic alterations may affect embryo viability and reproductive outcomes. Currently it is unknown whether embryo metabolism is affected by sperm-mediated abnormalities. Hence, using a mouse model, this study investigated the response to paternally transmitted DNA lesions on genetic integrity and metabolism in preimplantation embryos. Spent embryo culture media were analysed for metabolites by nuclear magnetic resonance spectroscopy and embryonic genetic integrity was determined by terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay on embryonic Day 4.5 (E4.5). Metabolic signatures were compared between normally derived embryos (control) and embryos derived from spermatozoa carrying induced DNA lesions (SDL). SDL embryos showed a significant reduction in blastocyst formation on E3.5 and E4.5 (P < 0.0001) and had an approximately 2-fold increase in TUNEL-positive cells (P < 0.01). A cohort of SDL embryos showing delayed development on E4.5 had increased uptake of pyruvate (P < 0.05) and released significantly less alanine (P < 0.05) to the medium compared with the corresponding control embryos. On the other hand, normally developed SDL embryos had a reduced (P < 0.001) pyruvate-to-alanine ratio compared with normally developed embryos from the control group. Hence, the difference in the metabolic behaviour of SDL embryos may be attributed to paternally transmitted DNA lesions in SDL embryos.

Original languageEnglish
Pages (from-to)443-450
Number of pages8
JournalReproduction, Fertility and Development
Volume31
Issue number3
DOIs
Publication statusPublished - 01-01-2019

Fingerprint

embryo culture
Culture Media
Spermatozoa
embryo (animal)
Embryonic Structures
culture media
spermatozoa
metabolites
DNA
Blastocyst
Pyruvic Acid
transferases
Alanine
alanine
metabolism
DNA Nucleotidylexotransferase
Transferases
blastocyst
Embryonic Development
nuclear magnetic resonance spectroscopy

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Reproductive Medicine
  • Animal Science and Zoology
  • Molecular Biology
  • Genetics
  • Endocrinology
  • Developmental Biology

Cite this

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title = "Sperm-mediated DNA lesions alter metabolite levels in spent embryo culture medium",
abstract = "Paternal genetic alterations may affect embryo viability and reproductive outcomes. Currently it is unknown whether embryo metabolism is affected by sperm-mediated abnormalities. Hence, using a mouse model, this study investigated the response to paternally transmitted DNA lesions on genetic integrity and metabolism in preimplantation embryos. Spent embryo culture media were analysed for metabolites by nuclear magnetic resonance spectroscopy and embryonic genetic integrity was determined by terminal deoxynucleotidyl transferase dUTP nick-end labelling (TUNEL) assay on embryonic Day 4.5 (E4.5). Metabolic signatures were compared between normally derived embryos (control) and embryos derived from spermatozoa carrying induced DNA lesions (SDL). SDL embryos showed a significant reduction in blastocyst formation on E3.5 and E4.5 (P < 0.0001) and had an approximately 2-fold increase in TUNEL-positive cells (P < 0.01). A cohort of SDL embryos showing delayed development on E4.5 had increased uptake of pyruvate (P < 0.05) and released significantly less alanine (P < 0.05) to the medium compared with the corresponding control embryos. On the other hand, normally developed SDL embryos had a reduced (P < 0.001) pyruvate-to-alanine ratio compared with normally developed embryos from the control group. Hence, the difference in the metabolic behaviour of SDL embryos may be attributed to paternally transmitted DNA lesions in SDL embryos.",
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Sperm-mediated DNA lesions alter metabolite levels in spent embryo culture medium. / Souza, Fiona D.; Asampille, Gitanjali; Uppangala, Shubhashree; Kalthur, Guruprasad; Atreya, Hanudatta S.; Adiga, Satish Kumar.

In: Reproduction, Fertility and Development, Vol. 31, No. 3, 01.01.2019, p. 443-450.

Research output: Contribution to journalArticle

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T1 - Sperm-mediated DNA lesions alter metabolite levels in spent embryo culture medium

AU - Souza, Fiona D.

AU - Asampille, Gitanjali

AU - Uppangala, Shubhashree

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AU - Atreya, Hanudatta S.

AU - Adiga, Satish Kumar

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