Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

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Abstract

Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. Results The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P<0.05), oligozoospermic (Plt;0.001) and teratozoospermic samples (Plt;0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Conclusions Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.

Original languageEnglish
Pages (from-to)557-563
Number of pages7
JournalJournal of Assisted Reproduction and Genetics
Volume29
Issue number6
DOIs
Publication statusPublished - 06-2012

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DNA Damage
Spermatozoa
In Situ Nick-End Labeling
Oligospermia
Transferases
Semen
Infertility
Chromatin
Pregnancy
Incidence

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

@article{61f5478052b5440982d83304f4142e9a,
title = "Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage",
abstract = "Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. Results The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P<0.05), oligozoospermic (Plt;0.001) and teratozoospermic samples (Plt;0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Conclusions Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.",
author = "Varshini Jayaraman and Dinesh Upadhya and Narayan, {Pratap Kumar} and Adiga, {Satish Kumar}",
year = "2012",
month = "6",
doi = "10.1007/s10815-012-9742-x",
language = "English",
volume = "29",
pages = "557--563",
journal = "Journal of Assisted Reproduction and Genetics",
issn = "1058-0468",
publisher = "Springer New York",
number = "6",

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TY - JOUR

T1 - Sperm processing by swim-up and density gradient is effective in elimination of sperm with DNA damage

AU - Jayaraman, Varshini

AU - Upadhya, Dinesh

AU - Narayan, Pratap Kumar

AU - Adiga, Satish Kumar

PY - 2012/6

Y1 - 2012/6

N2 - Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. Results The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P<0.05), oligozoospermic (Plt;0.001) and teratozoospermic samples (Plt;0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Conclusions Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.

AB - Purpose DNA damage may occur during sperm processing, thereby negatively influencing fertilizing ability of the sperm. The present study was designed to compare the effectiveness of gradient and swim-up, either alone or in combination, to eliminate sperm with DNA damage. Methods A total of 51 subjects visiting the University infertility clinic with normozoospermic parameters, oligozoospermia and teratozoospermia were included. Semen characteristics were analysed by standard criteria; Terminal deoxy nucelotidyl transferase mediated dUTP nick end labeling assay was employed for DNA damage assessment. Results The percentage of TUNEL positive sperm after sperm processing was significantly lower in normozoospermic (P<0.05), oligozoospermic (Plt;0.001) and teratozoospermic samples (Plt;0.01). No difference was observed in the incidence of TUNEL positive sperm between the various techniques, suggesting that they are comparable. Conclusions Sperm preparation has been found to result in enrichment of sperm with intact chromatin, which is likely to improve the chances of achieving a viable pregnancy.

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