Stability indicating assay method development and validation of naratriptan hydrochloride by RP–HPLC

M. Sai Vijay, D. Viswanath Guptha, B. S. Muddu Krishna, S. G. Vasantharaju

Research output: Contribution to journalArticle

Abstract

Analysis was performed using Gracesmart C18 column (250 × 4.5mm; 5μm). Isocratic mode of separation was followed using mobile phase which contains acetonitrile and phosphate buffer (pH adjusted to 4.0 by using ortho phosphoric acid) in proportion of 20:80 v/v. The developed method employs use of UV detector with detection wavelength of 225 nm, volume of injection was 20 μl and employing flow rate of 1ml/min. The Rt of the drug under study was found to be 6.0 ± 0.3mins. Method was precise, accurate, reproducible, linear and robust. The method was linear between 0.1 μg/ml to 25 μg/ml with r2 of 0.9991. The method would be applicable for assay of tablet dosage from, stability and dissolution of marketed Naratriptan hydrochloride tablets. The % assay for tablets using the proposed method was found to be 100.17. The % RSD from system and method precision were 0.48 and 0.79 respectively. Accuracy was found to be 100.5%.

Original languageEnglish
Pages (from-to)1177-1182
Number of pages6
JournalResearch Journal of Pharmacy and Technology
Volume9
Issue number8
DOIs
Publication statusPublished - 01-08-2016

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Tablets
naratriptan
Buffers
Phosphates
Injections
Pharmaceutical Preparations
phosphoric acid
acetonitrile

All Science Journal Classification (ASJC) codes

  • Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
  • Pharmacology (medical)

Cite this

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abstract = "Analysis was performed using Gracesmart C18 column (250 × 4.5mm; 5μm). Isocratic mode of separation was followed using mobile phase which contains acetonitrile and phosphate buffer (pH adjusted to 4.0 by using ortho phosphoric acid) in proportion of 20:80 v/v. The developed method employs use of UV detector with detection wavelength of 225 nm, volume of injection was 20 μl and employing flow rate of 1ml/min. The Rt of the drug under study was found to be 6.0 ± 0.3mins. Method was precise, accurate, reproducible, linear and robust. The method was linear between 0.1 μg/ml to 25 μg/ml with r2 of 0.9991. The method would be applicable for assay of tablet dosage from, stability and dissolution of marketed Naratriptan hydrochloride tablets. The {\%} assay for tablets using the proposed method was found to be 100.17. The {\%} RSD from system and method precision were 0.48 and 0.79 respectively. Accuracy was found to be 100.5{\%}.",
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Stability indicating assay method development and validation of naratriptan hydrochloride by RP–HPLC. / Sai Vijay, M.; Viswanath Guptha, D.; Muddu Krishna, B. S.; Vasantharaju, S. G.

In: Research Journal of Pharmacy and Technology, Vol. 9, No. 8, 01.08.2016, p. 1177-1182.

Research output: Contribution to journalArticle

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AU - Viswanath Guptha, D.

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AU - Vasantharaju, S. G.

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