Stability-indicating HPTLC determination of capsaicin in the bulk drug

Ganesa Sundararajan Subramanian, Arumugam Karthik, Suresh B. Kamath, Koiram Prabahar, Averineni Ranjithkumar, Shriram Pathak, Nayanabhirama Udupa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

A simple, selective, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method for analysis of capsaicin in the bulk drug has been developed and validated. The method employs aluminum TLC plates precoated with silica gel 60F254 as stationary phase. The mobile phase was toluene-ethyl acetate 6:4 (v/v). A compact spot was obtained for capsaicin (RF 0.38 ± 0.02). Densitometric analysis of capsaicin was performed in absorbance mode at 280 nm. Regression analysis of calibration data revealed a good linear relationship (r2 > 0.9936) for peak-area data in the concentration range 100-1000 ng per band. The method was validated for accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. The limits of detection and quantitation were 20 and 60 ng per band, respectively. Capsaicin was subjected to acidic and alkaline hydrolysis, oxidation, and thermal degradation. The drug undergoes degradation under acidic, basic, and oxidizing conditions and at elevated temperatures. Statistical analysis proved the method enables precise, selective, and accurate analysis of capsaicin. The method can be used for identification and quantitative analysis of capsaicin in the bulk drug.

Original languageEnglish
Pages (from-to)271-275
Number of pages5
JournalJournal of Planar Chromatography - Modern TLC
Volume21
Issue number4
DOIs
Publication statusPublished - 09-2008

Fingerprint

Capsaicin
Pharmaceutical Preparations
Limit of Detection
Silica Gel
Toluene
Thin Layer Chromatography
Aluminum
Regression analysis
Calibration
Hydrolysis
Statistical methods
Pyrolysis
Hot Temperature
Regression Analysis
Degradation
Oxidation
Temperature
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Subramanian, G. S., Karthik, A., Kamath, S. B., Prabahar, K., Ranjithkumar, A., Pathak, S., & Udupa, N. (2008). Stability-indicating HPTLC determination of capsaicin in the bulk drug. Journal of Planar Chromatography - Modern TLC, 21(4), 271-275. https://doi.org/10.1556/JPC.21.2008.4.9
Subramanian, Ganesa Sundararajan ; Karthik, Arumugam ; Kamath, Suresh B. ; Prabahar, Koiram ; Ranjithkumar, Averineni ; Pathak, Shriram ; Udupa, Nayanabhirama. / Stability-indicating HPTLC determination of capsaicin in the bulk drug. In: Journal of Planar Chromatography - Modern TLC. 2008 ; Vol. 21, No. 4. pp. 271-275.
@article{d7cd053470f74b1d93f07a56050355b3,
title = "Stability-indicating HPTLC determination of capsaicin in the bulk drug",
abstract = "A simple, selective, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method for analysis of capsaicin in the bulk drug has been developed and validated. The method employs aluminum TLC plates precoated with silica gel 60F254 as stationary phase. The mobile phase was toluene-ethyl acetate 6:4 (v/v). A compact spot was obtained for capsaicin (RF 0.38 ± 0.02). Densitometric analysis of capsaicin was performed in absorbance mode at 280 nm. Regression analysis of calibration data revealed a good linear relationship (r2 > 0.9936) for peak-area data in the concentration range 100-1000 ng per band. The method was validated for accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. The limits of detection and quantitation were 20 and 60 ng per band, respectively. Capsaicin was subjected to acidic and alkaline hydrolysis, oxidation, and thermal degradation. The drug undergoes degradation under acidic, basic, and oxidizing conditions and at elevated temperatures. Statistical analysis proved the method enables precise, selective, and accurate analysis of capsaicin. The method can be used for identification and quantitative analysis of capsaicin in the bulk drug.",
author = "Subramanian, {Ganesa Sundararajan} and Arumugam Karthik and Kamath, {Suresh B.} and Koiram Prabahar and Averineni Ranjithkumar and Shriram Pathak and Nayanabhirama Udupa",
year = "2008",
month = "9",
doi = "10.1556/JPC.21.2008.4.9",
language = "English",
volume = "21",
pages = "271--275",
journal = "Journal of Planar Chromatography - Modern TLC",
issn = "0933-4173",
publisher = "Research Institute for Medicinal Plants",
number = "4",

}

Subramanian, GS, Karthik, A, Kamath, SB, Prabahar, K, Ranjithkumar, A, Pathak, S & Udupa, N 2008, 'Stability-indicating HPTLC determination of capsaicin in the bulk drug', Journal of Planar Chromatography - Modern TLC, vol. 21, no. 4, pp. 271-275. https://doi.org/10.1556/JPC.21.2008.4.9

Stability-indicating HPTLC determination of capsaicin in the bulk drug. / Subramanian, Ganesa Sundararajan; Karthik, Arumugam; Kamath, Suresh B.; Prabahar, Koiram; Ranjithkumar, Averineni; Pathak, Shriram; Udupa, Nayanabhirama.

In: Journal of Planar Chromatography - Modern TLC, Vol. 21, No. 4, 09.2008, p. 271-275.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Stability-indicating HPTLC determination of capsaicin in the bulk drug

AU - Subramanian, Ganesa Sundararajan

AU - Karthik, Arumugam

AU - Kamath, Suresh B.

AU - Prabahar, Koiram

AU - Ranjithkumar, Averineni

AU - Pathak, Shriram

AU - Udupa, Nayanabhirama

PY - 2008/9

Y1 - 2008/9

N2 - A simple, selective, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method for analysis of capsaicin in the bulk drug has been developed and validated. The method employs aluminum TLC plates precoated with silica gel 60F254 as stationary phase. The mobile phase was toluene-ethyl acetate 6:4 (v/v). A compact spot was obtained for capsaicin (RF 0.38 ± 0.02). Densitometric analysis of capsaicin was performed in absorbance mode at 280 nm. Regression analysis of calibration data revealed a good linear relationship (r2 > 0.9936) for peak-area data in the concentration range 100-1000 ng per band. The method was validated for accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. The limits of detection and quantitation were 20 and 60 ng per band, respectively. Capsaicin was subjected to acidic and alkaline hydrolysis, oxidation, and thermal degradation. The drug undergoes degradation under acidic, basic, and oxidizing conditions and at elevated temperatures. Statistical analysis proved the method enables precise, selective, and accurate analysis of capsaicin. The method can be used for identification and quantitative analysis of capsaicin in the bulk drug.

AB - A simple, selective, precise, and stability-indicating high-performance thin-layer chromatographic (HPTLC) method for analysis of capsaicin in the bulk drug has been developed and validated. The method employs aluminum TLC plates precoated with silica gel 60F254 as stationary phase. The mobile phase was toluene-ethyl acetate 6:4 (v/v). A compact spot was obtained for capsaicin (RF 0.38 ± 0.02). Densitometric analysis of capsaicin was performed in absorbance mode at 280 nm. Regression analysis of calibration data revealed a good linear relationship (r2 > 0.9936) for peak-area data in the concentration range 100-1000 ng per band. The method was validated for accuracy, precision, linearity, limit of detection, limit of quantitation, and robustness. The limits of detection and quantitation were 20 and 60 ng per band, respectively. Capsaicin was subjected to acidic and alkaline hydrolysis, oxidation, and thermal degradation. The drug undergoes degradation under acidic, basic, and oxidizing conditions and at elevated temperatures. Statistical analysis proved the method enables precise, selective, and accurate analysis of capsaicin. The method can be used for identification and quantitative analysis of capsaicin in the bulk drug.

UR - http://www.scopus.com/inward/record.url?scp=51849152332&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=51849152332&partnerID=8YFLogxK

U2 - 10.1556/JPC.21.2008.4.9

DO - 10.1556/JPC.21.2008.4.9

M3 - Article

VL - 21

SP - 271

EP - 275

JO - Journal of Planar Chromatography - Modern TLC

JF - Journal of Planar Chromatography - Modern TLC

SN - 0933-4173

IS - 4

ER -

Subramanian GS, Karthik A, Kamath SB, Prabahar K, Ranjithkumar A, Pathak S et al. Stability-indicating HPTLC determination of capsaicin in the bulk drug. Journal of Planar Chromatography - Modern TLC. 2008 Sep;21(4):271-275. https://doi.org/10.1556/JPC.21.2008.4.9