Stability-indicating HPTLC method for analysis of tolterodine in the bulk drug

Akanksha Mishra, Vasantharaju Surenehalli Gowdra, Karthik Arumugam, Syed Sajjad Hussen, Krishnamoorthy Bhat, Nayanabhirama Udupa

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A simple, selective, precise, stability-indicating HPTLC method has been established for analysis of tolterodine in the bulk drug. Chromatography on aluminum foil-backed silica gel 60GF-254 plates with toluene-methanol-aqueous ammonia 5:5:0.02 (ν/ν) as mobile phase gave a compact spot at R F 0.40 ± 0.02. Densitometric analysis of tolterodine was performed in absorbance mode at 284 nm. Linear regression analysis of the calibration data revealed a good linear relationship (r2 > 0.990) between peak area and concentration in the range 200-1800 ng per band. The method was validated for accuracy, precision, linearity, limits of detection and quantification, and robustness. The limits of detection and quantification were 100 and 200 ng per band, respectively. Tolterodine was subjected to forced degradation and results showed the molecule undergoes degradation under basic conditions. Statistical analysis proved the method enables precise, selective, and accurate analysis of tolterodine. The method can be used for identification and quantitative analysis of tolterodine in the bulk drug.

Original languageEnglish
Pages (from-to)150-153
Number of pages4
JournalJournal of Planar Chromatography - Modern TLC
Volume24
Issue number2
DOIs
Publication statusPublished - 01-04-2011

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Pharmaceutical Preparations
Limit of Detection
Degradation
Aluminum foil
Silica Gel
Toluene
Chromatography
Aluminum
Ammonia
Linear regression
Regression analysis
Calibration
Methanol
Linear Models
Statistical methods
Regression Analysis
Tolterodine Tartrate
Molecules
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this

Mishra, Akanksha ; Gowdra, Vasantharaju Surenehalli ; Arumugam, Karthik ; Hussen, Syed Sajjad ; Bhat, Krishnamoorthy ; Udupa, Nayanabhirama. / Stability-indicating HPTLC method for analysis of tolterodine in the bulk drug. In: Journal of Planar Chromatography - Modern TLC. 2011 ; Vol. 24, No. 2. pp. 150-153.
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Stability-indicating HPTLC method for analysis of tolterodine in the bulk drug. / Mishra, Akanksha; Gowdra, Vasantharaju Surenehalli; Arumugam, Karthik; Hussen, Syed Sajjad; Bhat, Krishnamoorthy; Udupa, Nayanabhirama.

In: Journal of Planar Chromatography - Modern TLC, Vol. 24, No. 2, 01.04.2011, p. 150-153.

Research output: Contribution to journalArticle

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AU - Gowdra, Vasantharaju Surenehalli

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