Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy

Thiago De Oliveira Mendes, Liliane Pereira Pinto, Laurita Dos Santos, Vamshi Krishna Tippavajhala, Claudio Alberto Téllez Soto, Airton Abrahaõ Martin

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The analysis of biological systems by spectroscopic techniques involves the evaluation of hundreds to thousands of variables. Hence, different statistical approaches are used to elucidate regions that discriminate classes of samples and to propose new vibrational markers for explaining various phenomena like disease monitoring, mechanisms of action of drugs, food, and so on. However, the technical statistics are not always widely discussed in applied sciences. In this context, this work presents a detailed discussion including the various steps necessary for proper statistical analysis. It includes univariate parametric and nonparametric tests, as well as multivariate unsupervised and supervised approaches. The main objective of this study is to promote proper understanding of the application of various statistical tools in these spectroscopic methods used for the analysis of biological samples. The discussion of these methods is performed on a set of in vivo confocal Raman spectra of human skin analysis that aims to identify skin aging markers. In the Appendix, a complete routine of data analysis is executed in a free software that can be used by the scientific community involved in these studies.

Original languageEnglish
Article number075010
JournalJournal of Biomedical Optics
Volume21
Issue number7
DOIs
Publication statusPublished - 01-07-2016

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markers
Raman spectroscopy
Skin
Biological systems
food
statistical analysis
Raman scattering
Statistical methods
drugs
Aging of materials
Statistics
statistics
Raman spectra
computer programs
Monitoring
evaluation
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Mendes, Thiago De Oliveira ; Pinto, Liliane Pereira ; Santos, Laurita Dos ; Tippavajhala, Vamshi Krishna ; Soto, Claudio Alberto Téllez ; Martin, Airton Abrahaõ. / Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy. In: Journal of Biomedical Optics. 2016 ; Vol. 21, No. 7.
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Statistical strategies to reveal potential vibrational markers for in vivo analysis by confocal Raman spectroscopy. / Mendes, Thiago De Oliveira; Pinto, Liliane Pereira; Santos, Laurita Dos; Tippavajhala, Vamshi Krishna; Soto, Claudio Alberto Téllez; Martin, Airton Abrahaõ.

In: Journal of Biomedical Optics, Vol. 21, No. 7, 075010, 01.07.2016.

Research output: Contribution to journalArticle

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