Sterilisation of extracted human teeth for educational use

M. Kumar, P. S. Sequeira, S. Peter, G. K. Bhat

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10% formalin for seven days, group 2 teeth were immersed in 3% hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6% sodium hypochlorite for seven days, group 4 teeth were boiled in water at 100°C for 20 minutes, group 5 teeth were autoclaved at 121°C at 15 lbs psi for 30 minutes, and group 6 teeth were immersed in normal saline for seven days. After the treatment, the teeth were individually inoculated into trypticase soy broth and incubated for 48 hours. A questionnaire survey was also conducted to determine the awareness of dental students regarding infection due to extracted human teeth and the common disinfection/sterilisation methods used. Autoclaving at 121°C, 15 lbs psi for 30 minutes and immersion in 10% formalin for seven days were effective in disinfecting/sterilising extracted human teeth. Chemicals such as 2.6% sodium hypochlorite, 3% hydrogen peroxide and boiling in water were not effective. The results indicate that autoclaving for 30 minutes or immersion in 10% formalin for seven days could be effectively used for disinfection/sterilisation of extracted human teeth.

Original languageEnglish
Pages (from-to)256-258
Number of pages3
JournalIndian Journal of Medical Microbiology
Volume23
Issue number4
Publication statusPublished - 01-10-2005

Fingerprint

Tooth
Formaldehyde
Sodium Hypochlorite
Disinfection
Immersion
Hydrogen Peroxide
Dental Students
Water
Periodontal Diseases

All Science Journal Classification (ASJC) codes

  • Microbiology (medical)

Cite this

Kumar, M. ; Sequeira, P. S. ; Peter, S. ; Bhat, G. K. / Sterilisation of extracted human teeth for educational use. In: Indian Journal of Medical Microbiology. 2005 ; Vol. 23, No. 4. pp. 256-258.
@article{8b9b333b87704130bf42284559fb0b80,
title = "Sterilisation of extracted human teeth for educational use",
abstract = "Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10{\%} formalin for seven days, group 2 teeth were immersed in 3{\%} hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6{\%} sodium hypochlorite for seven days, group 4 teeth were boiled in water at 100°C for 20 minutes, group 5 teeth were autoclaved at 121°C at 15 lbs psi for 30 minutes, and group 6 teeth were immersed in normal saline for seven days. After the treatment, the teeth were individually inoculated into trypticase soy broth and incubated for 48 hours. A questionnaire survey was also conducted to determine the awareness of dental students regarding infection due to extracted human teeth and the common disinfection/sterilisation methods used. Autoclaving at 121°C, 15 lbs psi for 30 minutes and immersion in 10{\%} formalin for seven days were effective in disinfecting/sterilising extracted human teeth. Chemicals such as 2.6{\%} sodium hypochlorite, 3{\%} hydrogen peroxide and boiling in water were not effective. The results indicate that autoclaving for 30 minutes or immersion in 10{\%} formalin for seven days could be effectively used for disinfection/sterilisation of extracted human teeth.",
author = "M. Kumar and Sequeira, {P. S.} and S. Peter and Bhat, {G. K.}",
year = "2005",
month = "10",
day = "1",
language = "English",
volume = "23",
pages = "256--258",
journal = "Indian Journal of Medical Microbiology",
issn = "0255-0857",
publisher = "Medknow Publications and Media Pvt. Ltd",
number = "4",

}

Kumar, M, Sequeira, PS, Peter, S & Bhat, GK 2005, 'Sterilisation of extracted human teeth for educational use', Indian Journal of Medical Microbiology, vol. 23, no. 4, pp. 256-258.

Sterilisation of extracted human teeth for educational use. / Kumar, M.; Sequeira, P. S.; Peter, S.; Bhat, G. K.

In: Indian Journal of Medical Microbiology, Vol. 23, No. 4, 01.10.2005, p. 256-258.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sterilisation of extracted human teeth for educational use

AU - Kumar, M.

AU - Sequeira, P. S.

AU - Peter, S.

AU - Bhat, G. K.

PY - 2005/10/1

Y1 - 2005/10/1

N2 - Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10% formalin for seven days, group 2 teeth were immersed in 3% hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6% sodium hypochlorite for seven days, group 4 teeth were boiled in water at 100°C for 20 minutes, group 5 teeth were autoclaved at 121°C at 15 lbs psi for 30 minutes, and group 6 teeth were immersed in normal saline for seven days. After the treatment, the teeth were individually inoculated into trypticase soy broth and incubated for 48 hours. A questionnaire survey was also conducted to determine the awareness of dental students regarding infection due to extracted human teeth and the common disinfection/sterilisation methods used. Autoclaving at 121°C, 15 lbs psi for 30 minutes and immersion in 10% formalin for seven days were effective in disinfecting/sterilising extracted human teeth. Chemicals such as 2.6% sodium hypochlorite, 3% hydrogen peroxide and boiling in water were not effective. The results indicate that autoclaving for 30 minutes or immersion in 10% formalin for seven days could be effectively used for disinfection/sterilisation of extracted human teeth.

AB - Sixty intact, non-carious and unrestored teeth extracted due to periodontal disease were used to determine the most effective method of sterilisation. The teeth were divided into six groups, each containing 10 teeth. Group 1 teeth were immersed in 10% formalin for seven days, group 2 teeth were immersed in 3% hydrogen peroxide for seven days, group 3 teeth were immersed in 2.6% sodium hypochlorite for seven days, group 4 teeth were boiled in water at 100°C for 20 minutes, group 5 teeth were autoclaved at 121°C at 15 lbs psi for 30 minutes, and group 6 teeth were immersed in normal saline for seven days. After the treatment, the teeth were individually inoculated into trypticase soy broth and incubated for 48 hours. A questionnaire survey was also conducted to determine the awareness of dental students regarding infection due to extracted human teeth and the common disinfection/sterilisation methods used. Autoclaving at 121°C, 15 lbs psi for 30 minutes and immersion in 10% formalin for seven days were effective in disinfecting/sterilising extracted human teeth. Chemicals such as 2.6% sodium hypochlorite, 3% hydrogen peroxide and boiling in water were not effective. The results indicate that autoclaving for 30 minutes or immersion in 10% formalin for seven days could be effectively used for disinfection/sterilisation of extracted human teeth.

UR - http://www.scopus.com/inward/record.url?scp=28844448381&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28844448381&partnerID=8YFLogxK

M3 - Article

VL - 23

SP - 256

EP - 258

JO - Indian Journal of Medical Microbiology

JF - Indian Journal of Medical Microbiology

SN - 0255-0857

IS - 4

ER -