Stokes vector formalism based second harmonic generation microscopy

Jianjun Qiu; Nirmal Mazumder; Han Ruei Tsai; Chih Wei Hu; Fu Jen Kao

doi:10.1117/12.907199

Stokes vector formalism based second harmonic generation microscopy

Jianjun Qiu, Nirmal Mazumder, Han Ruei Tsai, Chih Wei Hu, Fu Jen Kao

Department of Biophysics, Manipal School of Life Sciences, Manipal

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

2 Citations (Scopus)

Abstract

In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

Original language	English
Title of host publication	Single Molecule Spectroscopy and Superresolution Imaging V
Volume	8228
DOIs	https://doi.org/10.1117/12.907199
Publication status	Published - 2012
Event	Single Molecule Spectroscopy and Superresolution Imaging V - San Francisco, CA, United States Duration: 21-01-2012 → 22-01-2012

Conference

Conference	Single Molecule Spectroscopy and Superresolution Imaging V
Country/Territory	United States
City	San Francisco, CA
Period	21-01-12 → 22-01-12

All Science Journal Classification (ASJC) codes

Atomic and Molecular Physics, and Optics
Electronic, Optical and Magnetic Materials
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.907199

Cite this

@inproceedings{c7a18fcf75a645a3b094db3e75973547,

title = "Stokes vector formalism based second harmonic generation microscopy",

abstract = "In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.",

author = "Jianjun Qiu and Nirmal Mazumder and Tsai, {Han Ruei} and Hu, {Chih Wei} and Kao, {Fu Jen}",

year = "2012",

doi = "10.1117/12.907199",

language = "English",

isbn = "9780819488718",

volume = "8228",

booktitle = "Single Molecule Spectroscopy and Superresolution Imaging V",

note = "Single Molecule Spectroscopy and Superresolution Imaging V ; Conference date: 21-01-2012 Through 22-01-2012",

}

TY - GEN

T1 - Stokes vector formalism based second harmonic generation microscopy

AU - Qiu, Jianjun

AU - Mazumder, Nirmal

AU - Tsai, Han Ruei

AU - Hu, Chih Wei

AU - Kao, Fu Jen

PY - 2012

Y1 - 2012

N2 - In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

AB - In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

UR - http://www.scopus.com/inward/record.url?scp=84857594622&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84857594622&partnerID=8YFLogxK

U2 - 10.1117/12.907199

DO - 10.1117/12.907199

M3 - Conference contribution

AN - SCOPUS:84857594622

SN - 9780819488718

VL - 8228

BT - Single Molecule Spectroscopy and Superresolution Imaging V

T2 - Single Molecule Spectroscopy and Superresolution Imaging V

Y2 - 21 January 2012 through 22 January 2012

ER -

Stokes vector formalism based second harmonic generation microscopy

Abstract

Conference

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this