Stokes vector formalism based second harmonic generation microscopy

Jianjun Qiu, Nirmal Mazumder, Han Ruei Tsai, Chih Wei Hu, Fu Jen Kao

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging V
Volume8228
DOIs
Publication statusPublished - 2012
EventSingle Molecule Spectroscopy and Superresolution Imaging V - San Francisco, CA, United States
Duration: 21-01-201222-01-2012

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging V
CountryUnited States
CitySan Francisco, CA
Period21-01-1222-01-12

Fingerprint

Birefringence
Biocompatible Materials
Harmonic generation
Microscopy
harmonic generations
Microscopic examination
Lasers
Collagen
formalism
microscopy
collagens
Biomaterials
Crystal orientation
birefringence
Microscopes
microscopes
Crystalline materials
Scanning
scanning
lasers

All Science Journal Classification (ASJC) codes

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Qiu, J., Mazumder, N., Tsai, H. R., Hu, C. W., & Kao, F. J. (2012). Stokes vector formalism based second harmonic generation microscopy. In Single Molecule Spectroscopy and Superresolution Imaging V (Vol. 8228). [82280C] https://doi.org/10.1117/12.907199
Qiu, Jianjun ; Mazumder, Nirmal ; Tsai, Han Ruei ; Hu, Chih Wei ; Kao, Fu Jen. / Stokes vector formalism based second harmonic generation microscopy. Single Molecule Spectroscopy and Superresolution Imaging V. Vol. 8228 2012.
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abstract = "In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.",
author = "Jianjun Qiu and Nirmal Mazumder and Tsai, {Han Ruei} and Hu, {Chih Wei} and Kao, {Fu Jen}",
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Qiu, J, Mazumder, N, Tsai, HR, Hu, CW & Kao, FJ 2012, Stokes vector formalism based second harmonic generation microscopy. in Single Molecule Spectroscopy and Superresolution Imaging V. vol. 8228, 82280C, Single Molecule Spectroscopy and Superresolution Imaging V, San Francisco, CA, United States, 21-01-12. https://doi.org/10.1117/12.907199

Stokes vector formalism based second harmonic generation microscopy. / Qiu, Jianjun; Mazumder, Nirmal; Tsai, Han Ruei; Hu, Chih Wei; Kao, Fu Jen.

Single Molecule Spectroscopy and Superresolution Imaging V. Vol. 8228 2012. 82280C.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

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AB - In this study, we have developed a four-channel Stokes vector formalism based second harmonic generation (SHG) microscopy to map and analyze SHG signal. A four-channel Stokesmeter setup is calibrated and integrated into a laser scanning microscope to measure and characterize the SH's corresponding Stokes parameters. We are demonstrating the use of SH and its Stokes parameters to visualize the birefringence and crystalline orientation of KDP and collagen. We believe the developed method can reveal unprecedented information for biomedical and biomaterial studies.

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Qiu J, Mazumder N, Tsai HR, Hu CW, Kao FJ. Stokes vector formalism based second harmonic generation microscopy. In Single Molecule Spectroscopy and Superresolution Imaging V. Vol. 8228. 2012. 82280C https://doi.org/10.1117/12.907199