Streptococcus pseudopneumoniae: an emerging respiratory tract pathogen

J Sariya Mohammadi, B Dhanashree

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

BACKGROUND & OBJECTIVES: Streptococcus pseudopneumoniae a member of the Viridans Streptococci, is known to be associated with chronic obstructive pulmonary disease and respiratory tract infections (RTI). Very scanty information is available on the isolation of S. pseudopneumoniae from India. Hence, the present study was an attempt to isolate S. pseudopneumoniae from clinical samples and to study their drug resistance pattern.

METHODS: Sputum samples (n=150) submitted to the microbiology laboratory for routine culture from patients clinically suspected to have lower respiratory tract infection were inoculated onto sheep blood agar and chocolate agar plates. Alpha haemolytic colonies were identified as S. pseudopneumoniae based on absence of capsule, bile solubility and optochin susceptibility in 5 per cent CO₂ and ambient air. Disk diffusion method was used for antibiotic susceptibilily testing.

RESULTS: Among the samples screened, 4 per cent showed the growth of only S. pseudopneumoniae. Other pathogens isolated were Streptococcus pneumoniae, Moraxella catarrhalis, Klebsiella spp., Enterococcus spp., Pseudomonas spp., Haemophilus influenzae, Staphylococcus aureus, Candida albicans. All the S. pseudopneumoniae isolates were resistant to erythromycin.

INTERPRETATION & CONCLUSIONS: Our preliminary results showed presence of S. pseudopneumoniae in this part of the country and these were associated with RTI. Currently, most clinical laboratories report optochin susceptible isolates in 5 per cent CO₂ as S. pneumoniae and the resistant ones are not further tested for susceptibility in ambient air. As a result, S. pseudopneumoniae may be missed out. Hence, performance of at least two tests, viz. optochin susceptibility with incubation in 5 per cent CO₂ and ambient air along with bile solubility is necessary to differentiate S. pneumoniae from S. pseudopneumoniae.

Original languageEnglish
Pages (from-to)877-80
Number of pages4
JournalIndian Journal of Medical Research
Volume136
Issue number5
Publication statusPublished - 11-2012

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Pathogens
Carbon Monoxide
Streptococcus pneumoniae
Streptococcus
Respiratory Tract Infections
Respiratory System
Air
Bile
Solubility
Agar
Clinical laboratories
Viridans Streptococci
Microbiology
Moraxella (Branhamella) catarrhalis
Pulmonary diseases
Klebsiella
Candida
Enterococcus
Haemophilus influenzae
Erythromycin

Cite this

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abstract = "BACKGROUND & OBJECTIVES: Streptococcus pseudopneumoniae a member of the Viridans Streptococci, is known to be associated with chronic obstructive pulmonary disease and respiratory tract infections (RTI). Very scanty information is available on the isolation of S. pseudopneumoniae from India. Hence, the present study was an attempt to isolate S. pseudopneumoniae from clinical samples and to study their drug resistance pattern.METHODS: Sputum samples (n=150) submitted to the microbiology laboratory for routine culture from patients clinically suspected to have lower respiratory tract infection were inoculated onto sheep blood agar and chocolate agar plates. Alpha haemolytic colonies were identified as S. pseudopneumoniae based on absence of capsule, bile solubility and optochin susceptibility in 5 per cent CO₂ and ambient air. Disk diffusion method was used for antibiotic susceptibilily testing.RESULTS: Among the samples screened, 4 per cent showed the growth of only S. pseudopneumoniae. Other pathogens isolated were Streptococcus pneumoniae, Moraxella catarrhalis, Klebsiella spp., Enterococcus spp., Pseudomonas spp., Haemophilus influenzae, Staphylococcus aureus, Candida albicans. All the S. pseudopneumoniae isolates were resistant to erythromycin.INTERPRETATION & CONCLUSIONS: Our preliminary results showed presence of S. pseudopneumoniae in this part of the country and these were associated with RTI. Currently, most clinical laboratories report optochin susceptible isolates in 5 per cent CO₂ as S. pneumoniae and the resistant ones are not further tested for susceptibility in ambient air. As a result, S. pseudopneumoniae may be missed out. Hence, performance of at least two tests, viz. optochin susceptibility with incubation in 5 per cent CO₂ and ambient air along with bile solubility is necessary to differentiate S. pneumoniae from S. pseudopneumoniae.",
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Streptococcus pseudopneumoniae : an emerging respiratory tract pathogen. / Mohammadi, J Sariya; Dhanashree, B.

In: Indian Journal of Medical Research, Vol. 136, No. 5, 11.2012, p. 877-80.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Streptococcus pseudopneumoniae

T2 - an emerging respiratory tract pathogen

AU - Mohammadi, J Sariya

AU - Dhanashree, B

PY - 2012/11

Y1 - 2012/11

N2 - BACKGROUND & OBJECTIVES: Streptococcus pseudopneumoniae a member of the Viridans Streptococci, is known to be associated with chronic obstructive pulmonary disease and respiratory tract infections (RTI). Very scanty information is available on the isolation of S. pseudopneumoniae from India. Hence, the present study was an attempt to isolate S. pseudopneumoniae from clinical samples and to study their drug resistance pattern.METHODS: Sputum samples (n=150) submitted to the microbiology laboratory for routine culture from patients clinically suspected to have lower respiratory tract infection were inoculated onto sheep blood agar and chocolate agar plates. Alpha haemolytic colonies were identified as S. pseudopneumoniae based on absence of capsule, bile solubility and optochin susceptibility in 5 per cent CO₂ and ambient air. Disk diffusion method was used for antibiotic susceptibilily testing.RESULTS: Among the samples screened, 4 per cent showed the growth of only S. pseudopneumoniae. Other pathogens isolated were Streptococcus pneumoniae, Moraxella catarrhalis, Klebsiella spp., Enterococcus spp., Pseudomonas spp., Haemophilus influenzae, Staphylococcus aureus, Candida albicans. All the S. pseudopneumoniae isolates were resistant to erythromycin.INTERPRETATION & CONCLUSIONS: Our preliminary results showed presence of S. pseudopneumoniae in this part of the country and these were associated with RTI. Currently, most clinical laboratories report optochin susceptible isolates in 5 per cent CO₂ as S. pneumoniae and the resistant ones are not further tested for susceptibility in ambient air. As a result, S. pseudopneumoniae may be missed out. Hence, performance of at least two tests, viz. optochin susceptibility with incubation in 5 per cent CO₂ and ambient air along with bile solubility is necessary to differentiate S. pneumoniae from S. pseudopneumoniae.

AB - BACKGROUND & OBJECTIVES: Streptococcus pseudopneumoniae a member of the Viridans Streptococci, is known to be associated with chronic obstructive pulmonary disease and respiratory tract infections (RTI). Very scanty information is available on the isolation of S. pseudopneumoniae from India. Hence, the present study was an attempt to isolate S. pseudopneumoniae from clinical samples and to study their drug resistance pattern.METHODS: Sputum samples (n=150) submitted to the microbiology laboratory for routine culture from patients clinically suspected to have lower respiratory tract infection were inoculated onto sheep blood agar and chocolate agar plates. Alpha haemolytic colonies were identified as S. pseudopneumoniae based on absence of capsule, bile solubility and optochin susceptibility in 5 per cent CO₂ and ambient air. Disk diffusion method was used for antibiotic susceptibilily testing.RESULTS: Among the samples screened, 4 per cent showed the growth of only S. pseudopneumoniae. Other pathogens isolated were Streptococcus pneumoniae, Moraxella catarrhalis, Klebsiella spp., Enterococcus spp., Pseudomonas spp., Haemophilus influenzae, Staphylococcus aureus, Candida albicans. All the S. pseudopneumoniae isolates were resistant to erythromycin.INTERPRETATION & CONCLUSIONS: Our preliminary results showed presence of S. pseudopneumoniae in this part of the country and these were associated with RTI. Currently, most clinical laboratories report optochin susceptible isolates in 5 per cent CO₂ as S. pneumoniae and the resistant ones are not further tested for susceptibility in ambient air. As a result, S. pseudopneumoniae may be missed out. Hence, performance of at least two tests, viz. optochin susceptibility with incubation in 5 per cent CO₂ and ambient air along with bile solubility is necessary to differentiate S. pneumoniae from S. pseudopneumoniae.

M3 - Article

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VL - 136

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EP - 880

JO - Indian Journal of Medical Research

JF - Indian Journal of Medical Research

SN - 0971-5916

IS - 5

ER -