Stroma formation and angiogenesis by overexpression of growth factors, cytokines, and proteolytic enzymes in human skin grafted to SCID mice

Claus J. Gruss, Kapaettu Satyamoorthy, Carola Berking, John Lininger, Mark Nesbit, Helmut Schaider, Zhao June Liu, Masahiro Oka, Mei Yu Hsu, Takashi Shirakawa, Gang Li, Thomas Bogenrieder, Peter Carmeliet, Wafik S. El-Deiry, Stephen L. Eck, Justi S. Rao, Andrew H. Baker, Jean T. Bennet, Timothy M. Crombleholme, Omaida Velazquez & 8 others Jagajan Karmacharya, David J. Margolis, James M. Wilson, Michael Detmar, Mihaela Skobe, Paul D. Robbins, Clayton Buck, Meenhard Herlyn

    Research output: Contribution to journalArticle

    38 Citations (Scopus)

    Abstract

    Reorganization of skin during wound healing, inflammatory disorders, or cancer growth is the result of expression changes of multiple genes associated with tissue morphogenesis. We wanted to identify proteins involved in skin remodeling and select those that may be targeted for agonistic or antagonist therapeutic approaches in various disease processes. Full-thickness human skin was grafted to severe combined immunodeficient mice and injected intradermally with 38 different adenoviral vectors inserted with 37 different genes coding for growth factors, cytokines, proteolytic enzymes and their inhibitors, adhesion receptors, oncogenes, and tumor suppressor genes. Responses were characterized for infiltration of inflammatory cells, vascular density, matrix formation, fibroblast-like cell proliferation, and epidermal hyperplasia. Of the 17 growth factor vectors, 16 induced histological changes in human skin. Members of the VEGF and angiopoietin families induced neovascularization. PDGFs and TGF-βs stimulated connective tissue formation, and the chemokines IL-8 and MCP-1 attracted inflammatory neutrophils and monocytes, respectively. The serine protease uPA induced a vascular response similar to that of VEGF. Vectors with adhesion receptors, oncogenes and tumor suppressor genes had, with few exceptions, little effects on skin architecture. The overall results suggest that adenoviral vectors can effectively remodel the architecture of human skin for studies in morphogenesis, inflammatory skin disorders, wound healing, and cancer development.

    Original languageEnglish
    Pages (from-to)683-692
    Number of pages10
    JournalJournal of Investigative Dermatology
    Volume120
    Issue number4
    DOIs
    Publication statusPublished - 01-04-2003

    Fingerprint

    SCID Mice
    Intercellular Signaling Peptides and Proteins
    Skin
    Peptide Hydrolases
    Cytokines
    Genes
    Tumor Suppressor Genes
    Morphogenesis
    Oncogenes
    Wound Healing
    Vascular Endothelial Growth Factor A
    Blood Vessels
    Tumors
    Angiopoietins
    Tissue
    Cell proliferation
    Serine Proteases
    Enzyme Inhibitors
    Fibroblasts
    Interleukin-8

    All Science Journal Classification (ASJC) codes

    • Biochemistry
    • Molecular Biology
    • Dermatology
    • Cell Biology

    Cite this

    Gruss, Claus J. ; Satyamoorthy, Kapaettu ; Berking, Carola ; Lininger, John ; Nesbit, Mark ; Schaider, Helmut ; Liu, Zhao June ; Oka, Masahiro ; Hsu, Mei Yu ; Shirakawa, Takashi ; Li, Gang ; Bogenrieder, Thomas ; Carmeliet, Peter ; El-Deiry, Wafik S. ; Eck, Stephen L. ; Rao, Justi S. ; Baker, Andrew H. ; Bennet, Jean T. ; Crombleholme, Timothy M. ; Velazquez, Omaida ; Karmacharya, Jagajan ; Margolis, David J. ; Wilson, James M. ; Detmar, Michael ; Skobe, Mihaela ; Robbins, Paul D. ; Buck, Clayton ; Herlyn, Meenhard. / Stroma formation and angiogenesis by overexpression of growth factors, cytokines, and proteolytic enzymes in human skin grafted to SCID mice. In: Journal of Investigative Dermatology. 2003 ; Vol. 120, No. 4. pp. 683-692.
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    abstract = "Reorganization of skin during wound healing, inflammatory disorders, or cancer growth is the result of expression changes of multiple genes associated with tissue morphogenesis. We wanted to identify proteins involved in skin remodeling and select those that may be targeted for agonistic or antagonist therapeutic approaches in various disease processes. Full-thickness human skin was grafted to severe combined immunodeficient mice and injected intradermally with 38 different adenoviral vectors inserted with 37 different genes coding for growth factors, cytokines, proteolytic enzymes and their inhibitors, adhesion receptors, oncogenes, and tumor suppressor genes. Responses were characterized for infiltration of inflammatory cells, vascular density, matrix formation, fibroblast-like cell proliferation, and epidermal hyperplasia. Of the 17 growth factor vectors, 16 induced histological changes in human skin. Members of the VEGF and angiopoietin families induced neovascularization. PDGFs and TGF-βs stimulated connective tissue formation, and the chemokines IL-8 and MCP-1 attracted inflammatory neutrophils and monocytes, respectively. The serine protease uPA induced a vascular response similar to that of VEGF. Vectors with adhesion receptors, oncogenes and tumor suppressor genes had, with few exceptions, little effects on skin architecture. The overall results suggest that adenoviral vectors can effectively remodel the architecture of human skin for studies in morphogenesis, inflammatory skin disorders, wound healing, and cancer development.",
    author = "Gruss, {Claus J.} and Kapaettu Satyamoorthy and Carola Berking and John Lininger and Mark Nesbit and Helmut Schaider and Liu, {Zhao June} and Masahiro Oka and Hsu, {Mei Yu} and Takashi Shirakawa and Gang Li and Thomas Bogenrieder and Peter Carmeliet and El-Deiry, {Wafik S.} and Eck, {Stephen L.} and Rao, {Justi S.} and Baker, {Andrew H.} and Bennet, {Jean T.} and Crombleholme, {Timothy M.} and Omaida Velazquez and Jagajan Karmacharya and Margolis, {David J.} and Wilson, {James M.} and Michael Detmar and Mihaela Skobe and Robbins, {Paul D.} and Clayton Buck and Meenhard Herlyn",
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    Gruss, CJ, Satyamoorthy, K, Berking, C, Lininger, J, Nesbit, M, Schaider, H, Liu, ZJ, Oka, M, Hsu, MY, Shirakawa, T, Li, G, Bogenrieder, T, Carmeliet, P, El-Deiry, WS, Eck, SL, Rao, JS, Baker, AH, Bennet, JT, Crombleholme, TM, Velazquez, O, Karmacharya, J, Margolis, DJ, Wilson, JM, Detmar, M, Skobe, M, Robbins, PD, Buck, C & Herlyn, M 2003, 'Stroma formation and angiogenesis by overexpression of growth factors, cytokines, and proteolytic enzymes in human skin grafted to SCID mice', Journal of Investigative Dermatology, vol. 120, no. 4, pp. 683-692. https://doi.org/10.1046/j.1523-1747.2003.12112.x

    Stroma formation and angiogenesis by overexpression of growth factors, cytokines, and proteolytic enzymes in human skin grafted to SCID mice. / Gruss, Claus J.; Satyamoorthy, Kapaettu; Berking, Carola; Lininger, John; Nesbit, Mark; Schaider, Helmut; Liu, Zhao June; Oka, Masahiro; Hsu, Mei Yu; Shirakawa, Takashi; Li, Gang; Bogenrieder, Thomas; Carmeliet, Peter; El-Deiry, Wafik S.; Eck, Stephen L.; Rao, Justi S.; Baker, Andrew H.; Bennet, Jean T.; Crombleholme, Timothy M.; Velazquez, Omaida; Karmacharya, Jagajan; Margolis, David J.; Wilson, James M.; Detmar, Michael; Skobe, Mihaela; Robbins, Paul D.; Buck, Clayton; Herlyn, Meenhard.

    In: Journal of Investigative Dermatology, Vol. 120, No. 4, 01.04.2003, p. 683-692.

    Research output: Contribution to journalArticle

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    AU - Gruss, Claus J.

    AU - Satyamoorthy, Kapaettu

    AU - Berking, Carola

    AU - Lininger, John

    AU - Nesbit, Mark

    AU - Schaider, Helmut

    AU - Liu, Zhao June

    AU - Oka, Masahiro

    AU - Hsu, Mei Yu

    AU - Shirakawa, Takashi

    AU - Li, Gang

    AU - Bogenrieder, Thomas

    AU - Carmeliet, Peter

    AU - El-Deiry, Wafik S.

    AU - Eck, Stephen L.

    AU - Rao, Justi S.

    AU - Baker, Andrew H.

    AU - Bennet, Jean T.

    AU - Crombleholme, Timothy M.

    AU - Velazquez, Omaida

    AU - Karmacharya, Jagajan

    AU - Margolis, David J.

    AU - Wilson, James M.

    AU - Detmar, Michael

    AU - Skobe, Mihaela

    AU - Robbins, Paul D.

    AU - Buck, Clayton

    AU - Herlyn, Meenhard

    PY - 2003/4/1

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