Strong Strand Breaks in DNA Induced by Thermal Energy Particles and Their Electrostatic Inhibition by Na + Nanostructures

Upendra Nayek, V. K. Unnikrishnan, Abdul Ajees Abdul Salam, Parinda Vasa, Santhosh Chidangil, Deepak Mathur

Research output: Contribution to journalArticle

Abstract

Low-power laser pulses of 6 ns duration (1064 nm wavelength) have been used to create plasma in an aqueous solution of plasmid DNA (pUC19). Thermal energy electrons and OH radicals in the plasma induce strand breakages in DNA, including double strand breaks and possible base oxidation/base degradation. The time evolution of these modifications shows that it takes barely 30 s for damage to DNA to occur. Addition of physiologically relevant concentrations of a salt (NaCl) significantly inhibits such damage. We rationalize such inhibition using simple electrostatic considerations. The observation that DNA damage is induced by plasma-induced photolysis of water suggests implications beyond studies of DNA and opens new vistas for using simple nanosecond lasers to probe how ultralow energy radiation may affect living matter under physiological conditions.

Original languageEnglish
Pages (from-to)3141-3247
Number of pages107
JournalJournal of Physical Chemistry A
Volume123
Issue number15
DOIs
Publication statusPublished - 18-04-2019

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Thermal energy
thermal energy
strands
Electrostatics
Nanostructures
deoxyribonucleic acid
electrostatics
DNA
damage
Plasmas
plasmids
Photolysis
lasers
photolysis
Laser pulses
Plasmids
Salts
degradation
aqueous solutions
salts

All Science Journal Classification (ASJC) codes

  • Physical and Theoretical Chemistry

Cite this

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abstract = "Low-power laser pulses of 6 ns duration (1064 nm wavelength) have been used to create plasma in an aqueous solution of plasmid DNA (pUC19). Thermal energy electrons and OH radicals in the plasma induce strand breakages in DNA, including double strand breaks and possible base oxidation/base degradation. The time evolution of these modifications shows that it takes barely 30 s for damage to DNA to occur. Addition of physiologically relevant concentrations of a salt (NaCl) significantly inhibits such damage. We rationalize such inhibition using simple electrostatic considerations. The observation that DNA damage is induced by plasma-induced photolysis of water suggests implications beyond studies of DNA and opens new vistas for using simple nanosecond lasers to probe how ultralow energy radiation may affect living matter under physiological conditions.",
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Strong Strand Breaks in DNA Induced by Thermal Energy Particles and Their Electrostatic Inhibition by Na + Nanostructures. / Nayek, Upendra; Unnikrishnan, V. K.; Abdul Salam, Abdul Ajees; Vasa, Parinda; Chidangil, Santhosh; Mathur, Deepak.

In: Journal of Physical Chemistry A, Vol. 123, No. 15, 18.04.2019, p. 3141-3247.

Research output: Contribution to journalArticle

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