TY - JOUR
T1 - Structural characterization of collagen using polarization-resolved second harmonic generation (SHG) microscopy
AU - Mazumder, N.
AU - Sindhoora, K. M.
AU - Kao, F. J.
N1 - Funding Information:
We thank the Department of Science and Technology (Project No.: DST/INT//BLG/P-03/2019), Government of India for the funding. We thank Manipal School of Life Sciences, Manipal Academy of Higher Education, Karnataka, Manipal, India for providing the infrastructure during the preparation of the manuscript. The authors also thank the National Science Council, Taiwan (NSC99-2627-M-010-002, NSC98-2627-M-010-006, NSC97-2112-M-010-002-MY3, and NSC98-2112-M-010-001-MY3).
Publisher Copyright:
© 2021 Published under licence by IOP Publishing Ltd.
PY - 2021/4/9
Y1 - 2021/4/9
N2 - Second harmonic generation (SHG) microscopy is an effective analytical tool for a detailed investigation of the microscopic structure of non-centrosymmetric molecules. We developed a four-channel photon-counting-based Stokes polarimeter integrated to the SHG microscope for spatial characterization of polarization effects in the SH signal. We implemented Stokes-vector-based polarization-resolved SHG imaging to perform quantitative polarimetry, with a view to applications in biomedicine, which can measure the full polarization state of the SH light. In this work, we describe the application of a Stokes-vector-based four-channel photon counting SHG microscope to determine the molecular interpretation of the SH light from collagen in normal, scar, and keloid tissue.
AB - Second harmonic generation (SHG) microscopy is an effective analytical tool for a detailed investigation of the microscopic structure of non-centrosymmetric molecules. We developed a four-channel photon-counting-based Stokes polarimeter integrated to the SHG microscope for spatial characterization of polarization effects in the SH signal. We implemented Stokes-vector-based polarization-resolved SHG imaging to perform quantitative polarimetry, with a view to applications in biomedicine, which can measure the full polarization state of the SH light. In this work, we describe the application of a Stokes-vector-based four-channel photon counting SHG microscope to determine the molecular interpretation of the SH light from collagen in normal, scar, and keloid tissue.
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U2 - 10.1088/1742-6596/1859/1/012034
DO - 10.1088/1742-6596/1859/1/012034
M3 - Conference article
AN - SCOPUS:85104248541
SN - 1742-6588
VL - 1859
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012034
T2 - 21st International Conference and School on Quantum Electronics: Laser Physics and Applications, ICSQE 2020
Y2 - 21 September 2020 through 25 September 2020
ER -