Structural characterization of collagen using polarization-resolved second harmonic generation (SHG) microscopy

N. Mazumder, K. M. Sindhoora, F. J. Kao

Research output: Contribution to journalConference articlepeer-review

Abstract

Second harmonic generation (SHG) microscopy is an effective analytical tool for a detailed investigation of the microscopic structure of non-centrosymmetric molecules. We developed a four-channel photon-counting-based Stokes polarimeter integrated to the SHG microscope for spatial characterization of polarization effects in the SH signal. We implemented Stokes-vector-based polarization-resolved SHG imaging to perform quantitative polarimetry, with a view to applications in biomedicine, which can measure the full polarization state of the SH light. In this work, we describe the application of a Stokes-vector-based four-channel photon counting SHG microscope to determine the molecular interpretation of the SH light from collagen in normal, scar, and keloid tissue.

Original languageEnglish
Article number012034
JournalJournal of Physics: Conference Series
Volume1859
Issue number1
DOIs
Publication statusPublished - 09-04-2021
Event21st International Conference and School on Quantum Electronics: Laser Physics and Applications, ICSQE 2020 - Virtual, Online
Duration: 21-09-202025-09-2020

All Science Journal Classification (ASJC) codes

  • Physics and Astronomy(all)

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