Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes

Chethana Chetan, Ashok Prabhu, Vivian D'Souza, Shrinivas B. Rao

Research output: Contribution to journalArticle

Abstract

Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against the peroxidative action of H2O2which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H2O2 and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines may have independent mechanism of action.

Original languageEnglish
Pages (from-to)17-21
Number of pages5
JournalIndian Journal of Clinical Biochemistry
Volume15
Issue number1
DOIs
Publication statusPublished - 01-01-2000

Fingerprint

Polyamines
Putrescine
Hemolysis
Erythrocytes
Spermidine
Spermine
Antioxidants
Blood

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

Chetan, Chethana ; Prabhu, Ashok ; D'Souza, Vivian ; Rao, Shrinivas B. / Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes. In: Indian Journal of Clinical Biochemistry. 2000 ; Vol. 15, No. 1. pp. 17-21.
@article{7e85da21a7874e2cad7c81eb7a0a53d0,
title = "Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes",
abstract = "Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against the peroxidative action of H2O2which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H2O2 and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines may have independent mechanism of action.",
author = "Chethana Chetan and Ashok Prabhu and Vivian D'Souza and Rao, {Shrinivas B.}",
year = "2000",
month = "1",
day = "1",
doi = "10.1007/BF02873542",
language = "English",
volume = "15",
pages = "17--21",
journal = "Indian Journal of Clinical Biochemistry",
issn = "0970-1915",
publisher = "Springer India",
number = "1",

}

Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes. / Chetan, Chethana; Prabhu, Ashok; D'Souza, Vivian; Rao, Shrinivas B.

In: Indian Journal of Clinical Biochemistry, Vol. 15, No. 1, 01.01.2000, p. 17-21.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Studies on the antiperoxide and antihaemolytic action of polyamines using human erythrocytes

AU - Chetan, Chethana

AU - Prabhu, Ashok

AU - D'Souza, Vivian

AU - Rao, Shrinivas B.

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against the peroxidative action of H2O2which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H2O2 and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines may have independent mechanism of action.

AB - Blood RBC's were used to study the role of polyamines, putrescine, spermidine and spermine individually as antioxidants against the peroxidative action of H2O2which leads to haemolysis of erythrocytes. Results indicated that putrescine did not act as antioxidant at all concentrations used in our studies. However, spermidine and spermine suppressed peroxidative changes in RBC's due to the action of H2O2 and this suppression was greater with higher concentration of these individual polyamines used, separately. Further, all the three polyamines decreased haemolysis of erythrocytes at all concentrations studied. The action of putrescine to suppress haemolysis of RBC's without showing antioxidant property hypothetically suggests that these properties of respective polyamines may have independent mechanism of action.

UR - http://www.scopus.com/inward/record.url?scp=77950098380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77950098380&partnerID=8YFLogxK

U2 - 10.1007/BF02873542

DO - 10.1007/BF02873542

M3 - Article

VL - 15

SP - 17

EP - 21

JO - Indian Journal of Clinical Biochemistry

JF - Indian Journal of Clinical Biochemistry

SN - 0970-1915

IS - 1

ER -