Study of binding and denaturation dynamics of IgG and anti-IgG using dual color fluorescence correlation spectroscopy

Leo Tom Varghese, Rajeev K. Sinha, Joseph Irudayaraj

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In this article, we present a systematic study on IgG and Fab fragment of anti-IgG molecules using fluorescence auto- and cross-correlation spectroscopy to investigate their diffusion characteristics, binding kinetics, and the effect of small organic molecule, urea on their binding. Through our analysis, we found that the diffusion coefficient for IgG and Fab fragment of anti-IgG molecules were 37 ± 2 μm2 s-1 and 56 ± 2 μm2 s-1, respectively. From the binding kinetics study, the respective forward (ka) and backward (kd) reaction rates were (5.25 ± 0.25) × 106 M-1 s-1 and 0.08 ± 0.005 s-1, respectively and the corresponding dissociation binding constant (KD) was 15 ± 2 nM. We also found that urea inhibits the binding of these molecules at 4 M concentration due to denaturation.

Original languageEnglish
Pages (from-to)103-109
Number of pages7
JournalAnalytica Chimica Acta
Volume625
Issue number1
DOIs
Publication statusPublished - 05-09-2008

Fingerprint

Immunoglobulin Fab Fragments
Denaturation
Fluorescence Spectrometry
urea
Urea
fluorescence
Color
Immunoglobulin G
Fluorescence
spectroscopy
Spectroscopy
kinetics
Molecules
reaction rate
autocorrelation
Spectrum Analysis
Kinetics
Autocorrelation
Reaction rates
anti-IgG

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Analytical Chemistry
  • Spectroscopy
  • Environmental Chemistry

Cite this

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abstract = "In this article, we present a systematic study on IgG and Fab fragment of anti-IgG molecules using fluorescence auto- and cross-correlation spectroscopy to investigate their diffusion characteristics, binding kinetics, and the effect of small organic molecule, urea on their binding. Through our analysis, we found that the diffusion coefficient for IgG and Fab fragment of anti-IgG molecules were 37 ± 2 μm2 s-1 and 56 ± 2 μm2 s-1, respectively. From the binding kinetics study, the respective forward (ka) and backward (kd) reaction rates were (5.25 ± 0.25) × 106 M-1 s-1 and 0.08 ± 0.005 s-1, respectively and the corresponding dissociation binding constant (KD) was 15 ± 2 nM. We also found that urea inhibits the binding of these molecules at 4 M concentration due to denaturation.",
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Study of binding and denaturation dynamics of IgG and anti-IgG using dual color fluorescence correlation spectroscopy. / Varghese, Leo Tom; Sinha, Rajeev K.; Irudayaraj, Joseph.

In: Analytica Chimica Acta, Vol. 625, No. 1, 05.09.2008, p. 103-109.

Research output: Contribution to journalArticle

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