Studying cytotoxicity of low concentration arsenate and arsenite on IEC-6 cells

S. A. Salmataj, Shobha Kamath, Ramachandramurty

Research output: Contribution to journalArticle

Abstract

Chronic exposure to low concentration arsenic species in food and water poses a health risk. Complex process is involved in absorption of arsenic through intestinal epithelial cells. Herein, IEC-6 (rat intestinal epithelial cell line) cells has been selected to evaluate the toxicological effect of NaAsO2(As III) and Na3AsO4(As V) on growth and function of intestinal epithelial cells. Half maximal inhibition concentration (IC50) of NaAsO2(12mM) and Na3AsO4(48 mM) of 24 hr has been obtained from MTT assay. Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. The current study demonstrates that trivalent (As III) oxidation state induces ROS, genotoxicity and alters mitochondrial membrane potential (Øm). Whereas pentavalent (AsV) oxidation state induces cytotoxicity and fails to generate genotoxicity at low concentrations. (i.e., 10% of IC50 value) in IEC-6 cells.

Original languageEnglish
Pages (from-to)603-610
Number of pages8
JournalAsian Journal of Microbiology, Biotechnology and Environmental Sciences
Volume20
Issue number2
Publication statusPublished - 01-01-2018

Fingerprint

arsenite
genotoxicity
arsenate
Epithelial Cells
Arsenic
Inhibitory Concentration 50
arsenic
oxidation
Mitochondrial Membrane Potential
Toxicology
health risk
assay
membrane
Cell Line
Food
food
Water
Health
Growth
cytotoxicity

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Microbiology
  • Applied Microbiology and Biotechnology
  • Environmental Science(all)

Cite this

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abstract = "Chronic exposure to low concentration arsenic species in food and water poses a health risk. Complex process is involved in absorption of arsenic through intestinal epithelial cells. Herein, IEC-6 (rat intestinal epithelial cell line) cells has been selected to evaluate the toxicological effect of NaAsO2(As III) and Na3AsO4(As V) on growth and function of intestinal epithelial cells. Half maximal inhibition concentration (IC50) of NaAsO2(12mM) and Na3AsO4(48 mM) of 24 hr has been obtained from MTT assay. Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. The current study demonstrates that trivalent (As III) oxidation state induces ROS, genotoxicity and alters mitochondrial membrane potential ({\O}m). Whereas pentavalent (AsV) oxidation state induces cytotoxicity and fails to generate genotoxicity at low concentrations. (i.e., 10{\%} of IC50 value) in IEC-6 cells.",
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Studying cytotoxicity of low concentration arsenate and arsenite on IEC-6 cells. / Salmataj, S. A.; Kamath, Shobha; Ramachandramurty.

In: Asian Journal of Microbiology, Biotechnology and Environmental Sciences, Vol. 20, No. 2, 01.01.2018, p. 603-610.

Research output: Contribution to journalArticle

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AB - Chronic exposure to low concentration arsenic species in food and water poses a health risk. Complex process is involved in absorption of arsenic through intestinal epithelial cells. Herein, IEC-6 (rat intestinal epithelial cell line) cells has been selected to evaluate the toxicological effect of NaAsO2(As III) and Na3AsO4(As V) on growth and function of intestinal epithelial cells. Half maximal inhibition concentration (IC50) of NaAsO2(12mM) and Na3AsO4(48 mM) of 24 hr has been obtained from MTT assay. Cytotoxicity and genotoxicity has beenevaluated by exposure of IEC-6 cells to arsenate (5mM)and arsenite (1.5mM)at low concentrations. The current study demonstrates that trivalent (As III) oxidation state induces ROS, genotoxicity and alters mitochondrial membrane potential (Øm). Whereas pentavalent (AsV) oxidation state induces cytotoxicity and fails to generate genotoxicity at low concentrations. (i.e., 10% of IC50 value) in IEC-6 cells.

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