Sunscreen creams containing naringenin nanoparticles: Formulation development and in vitro and in vivo evaluations

Haritima Joshi, Aswathi R. Hegde, Pallavi K. Shetty, Hemanth Gollavilli, Renuka S. Managuli, Guruprasad Kalthur, Srinivas Mutalik

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: The aim of this study was to develop sunscreen creams containing polymeric nanoparticles (NPs) of naringenin for photoprotective and antioxidant effects. Methods: Polymeric NPs of naringenin were prepared and optimized. The NPs were incorporated into sunscreen creams and evaluated for in vitro and in vivo skin retention. Results: The optimized naringenin NPs showed a size of 131.2 nm, zeta potential −25.4 mV, and entrapment efficiency 32.45%. The absence of drug-excipient interaction was confirmed by Fourier transform infrared spectroscopy and differential scanning calorimetry. X-Ray diffraction analysis demonstrated the amorphization of naringenin in nanoparticles. Transmission electron microscopy showed the sphericity of the NPs with the size of <200 nm. Cytotoxicity assessment in HaCaT cells indicated non-toxic nature of naringenin NPs. In vitro skin permeation studies demonstrated that higher amount of naringenin permeated at the end of 12 hours (Q12 hours = 184.03 ± 3.37 μg/cm2) and deposited in the skin (10.38 ± 0.48 μg/cm2) from NPs as compared to plain naringenin. Sunscreen creams (SC1-SC5) containing plain naringenin or NPs with/without nano-zinc oxide and nano-titanium dioxide were prepared and evaluated. Optimized cream (SC5) containing naringenin NPs showed highest SPF value and enhanced skin retention of naringenin in comparison with NPs in suspension form and other cream formulations. Conclusion: Optimized nanoparticulate sunscreen cream exhibited highest skin retention and negligible skin permeation of naringenin besides showing excellent SPF value.

Original languageEnglish
Pages (from-to)69-81
Number of pages13
JournalPhotodermatology Photoimmunology and Photomedicine
Volume34
Issue number1
DOIs
Publication statusPublished - 01-01-2018

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Sunscreening Agents
Nanoparticles
Skin
In Vitro Techniques
naringenin
Zinc Oxide
Excipients
Differential Scanning Calorimetry
Fourier Transform Infrared Spectroscopy
Transmission Electron Microscopy
Drug Interactions
X-Ray Diffraction
Suspensions
Antioxidants

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Radiology Nuclear Medicine and imaging
  • Dermatology

Cite this

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title = "Sunscreen creams containing naringenin nanoparticles: Formulation development and in vitro and in vivo evaluations",
abstract = "Background: The aim of this study was to develop sunscreen creams containing polymeric nanoparticles (NPs) of naringenin for photoprotective and antioxidant effects. Methods: Polymeric NPs of naringenin were prepared and optimized. The NPs were incorporated into sunscreen creams and evaluated for in vitro and in vivo skin retention. Results: The optimized naringenin NPs showed a size of 131.2 nm, zeta potential −25.4 mV, and entrapment efficiency 32.45{\%}. The absence of drug-excipient interaction was confirmed by Fourier transform infrared spectroscopy and differential scanning calorimetry. X-Ray diffraction analysis demonstrated the amorphization of naringenin in nanoparticles. Transmission electron microscopy showed the sphericity of the NPs with the size of <200 nm. Cytotoxicity assessment in HaCaT cells indicated non-toxic nature of naringenin NPs. In vitro skin permeation studies demonstrated that higher amount of naringenin permeated at the end of 12 hours (Q12 hours = 184.03 ± 3.37 μg/cm2) and deposited in the skin (10.38 ± 0.48 μg/cm2) from NPs as compared to plain naringenin. Sunscreen creams (SC1-SC5) containing plain naringenin or NPs with/without nano-zinc oxide and nano-titanium dioxide were prepared and evaluated. Optimized cream (SC5) containing naringenin NPs showed highest SPF value and enhanced skin retention of naringenin in comparison with NPs in suspension form and other cream formulations. Conclusion: Optimized nanoparticulate sunscreen cream exhibited highest skin retention and negligible skin permeation of naringenin besides showing excellent SPF value.",
author = "Haritima Joshi and Hegde, {Aswathi R.} and Shetty, {Pallavi K.} and Hemanth Gollavilli and Managuli, {Renuka S.} and Guruprasad Kalthur and Srinivas Mutalik",
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Sunscreen creams containing naringenin nanoparticles : Formulation development and in vitro and in vivo evaluations. / Joshi, Haritima; Hegde, Aswathi R.; Shetty, Pallavi K.; Gollavilli, Hemanth; Managuli, Renuka S.; Kalthur, Guruprasad; Mutalik, Srinivas.

In: Photodermatology Photoimmunology and Photomedicine, Vol. 34, No. 1, 01.01.2018, p. 69-81.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Sunscreen creams containing naringenin nanoparticles

T2 - Formulation development and in vitro and in vivo evaluations

AU - Joshi, Haritima

AU - Hegde, Aswathi R.

AU - Shetty, Pallavi K.

AU - Gollavilli, Hemanth

AU - Managuli, Renuka S.

AU - Kalthur, Guruprasad

AU - Mutalik, Srinivas

PY - 2018/1/1

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N2 - Background: The aim of this study was to develop sunscreen creams containing polymeric nanoparticles (NPs) of naringenin for photoprotective and antioxidant effects. Methods: Polymeric NPs of naringenin were prepared and optimized. The NPs were incorporated into sunscreen creams and evaluated for in vitro and in vivo skin retention. Results: The optimized naringenin NPs showed a size of 131.2 nm, zeta potential −25.4 mV, and entrapment efficiency 32.45%. The absence of drug-excipient interaction was confirmed by Fourier transform infrared spectroscopy and differential scanning calorimetry. X-Ray diffraction analysis demonstrated the amorphization of naringenin in nanoparticles. Transmission electron microscopy showed the sphericity of the NPs with the size of <200 nm. Cytotoxicity assessment in HaCaT cells indicated non-toxic nature of naringenin NPs. In vitro skin permeation studies demonstrated that higher amount of naringenin permeated at the end of 12 hours (Q12 hours = 184.03 ± 3.37 μg/cm2) and deposited in the skin (10.38 ± 0.48 μg/cm2) from NPs as compared to plain naringenin. Sunscreen creams (SC1-SC5) containing plain naringenin or NPs with/without nano-zinc oxide and nano-titanium dioxide were prepared and evaluated. Optimized cream (SC5) containing naringenin NPs showed highest SPF value and enhanced skin retention of naringenin in comparison with NPs in suspension form and other cream formulations. Conclusion: Optimized nanoparticulate sunscreen cream exhibited highest skin retention and negligible skin permeation of naringenin besides showing excellent SPF value.

AB - Background: The aim of this study was to develop sunscreen creams containing polymeric nanoparticles (NPs) of naringenin for photoprotective and antioxidant effects. Methods: Polymeric NPs of naringenin were prepared and optimized. The NPs were incorporated into sunscreen creams and evaluated for in vitro and in vivo skin retention. Results: The optimized naringenin NPs showed a size of 131.2 nm, zeta potential −25.4 mV, and entrapment efficiency 32.45%. The absence of drug-excipient interaction was confirmed by Fourier transform infrared spectroscopy and differential scanning calorimetry. X-Ray diffraction analysis demonstrated the amorphization of naringenin in nanoparticles. Transmission electron microscopy showed the sphericity of the NPs with the size of <200 nm. Cytotoxicity assessment in HaCaT cells indicated non-toxic nature of naringenin NPs. In vitro skin permeation studies demonstrated that higher amount of naringenin permeated at the end of 12 hours (Q12 hours = 184.03 ± 3.37 μg/cm2) and deposited in the skin (10.38 ± 0.48 μg/cm2) from NPs as compared to plain naringenin. Sunscreen creams (SC1-SC5) containing plain naringenin or NPs with/without nano-zinc oxide and nano-titanium dioxide were prepared and evaluated. Optimized cream (SC5) containing naringenin NPs showed highest SPF value and enhanced skin retention of naringenin in comparison with NPs in suspension form and other cream formulations. Conclusion: Optimized nanoparticulate sunscreen cream exhibited highest skin retention and negligible skin permeation of naringenin besides showing excellent SPF value.

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