Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development

Sujith Raj Salian, Guruprasad Nayak, Sandhya Kumari, Sandesh Patel, Shruthi Gowda, Yashaswini Shenoy, Sinoy Sugunan, Rajanikant G.K, Renuka Suresh Managuli, Srinivas Mutalik, Vandana Dahiya, Samanwita Pal, Satish Kumar Adiga, Guruprasad Kalthur

Research output: Contribution to journalArticle

Abstract

Purpose: Motility of spermatozoa helps not only in planning the type of infertility treatment but also directly reflects the success rate in assisted reproductive technology (ART). Previously, biotin, a water-soluble vitamin, has been shown to increase the motility and longevity of cryopreserved human spermatozoa. The present study was designed to understand the molecular basis of the beneficial effects of presence of biotin in sperm wash medium on early embryo development. Methods: The effect biotin supplementation to sperm wash medium on the sperm parameters were assessed in swim-up fraction of normozoospermic and asthenozoospermic ejaculates collected from infertile men. Fertilization and early embryo development was studied using Swiss albino mice. Results: Even though both biotin and pentoxifylline (PTX) enhanced the motility of spermatozoa from normozoospermic and asthenozoospermic samples, biotin group exhibited higher in vitro survival. Using mouse model, we observed that presence of biotin or PTX in sperm wash medium improved the fertilization rate and blastocyst rate compared to control. Blastocysts from these groups had significantly higher total cell number (P < 0.01) and lower apoptotic index. In silico target prediction revealed that GTPase HRas (HRas), tyrosine-protein phosphatase nonreceptor type 1 (PTP1B), and glucokinase are the probable targets for biotin. Solution-state Nuclear Magnetic Resonance (NMR) studies confirmed that biotin interacts both with human HRas and PTP1B. Conclusion: Our results indicate that presence of biotin in sperm wash medium can improve the fertilization potential and preimplantation embryo development and can be considered as a safe alternate to PTX.

Original languageEnglish
Pages (from-to)255-266
Number of pages12
JournalJournal of Assisted Reproduction and Genetics
Volume36
Issue number2
DOIs
Publication statusPublished - 15-02-2019

Fingerprint

Biotin
Embryonic Development
Spermatozoa
Pentoxifylline
Fertilization
Blastocyst
Glucokinase
Protein Phosphatase 1
Assisted Reproductive Techniques
GTP Phosphohydrolases
Vitamins
Computer Simulation
Infertility
Tyrosine
Magnetic Resonance Spectroscopy
Cell Count
Survival
Water

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

Salian, Sujith Raj ; Nayak, Guruprasad ; Kumari, Sandhya ; Patel, Sandesh ; Gowda, Shruthi ; Shenoy, Yashaswini ; Sugunan, Sinoy ; G.K, Rajanikant ; Managuli, Renuka Suresh ; Mutalik, Srinivas ; Dahiya, Vandana ; Pal, Samanwita ; Adiga, Satish Kumar ; Kalthur, Guruprasad. / Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development. In: Journal of Assisted Reproduction and Genetics. 2019 ; Vol. 36, No. 2. pp. 255-266.
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Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development. / Salian, Sujith Raj; Nayak, Guruprasad; Kumari, Sandhya; Patel, Sandesh; Gowda, Shruthi; Shenoy, Yashaswini; Sugunan, Sinoy; G.K, Rajanikant; Managuli, Renuka Suresh; Mutalik, Srinivas; Dahiya, Vandana; Pal, Samanwita; Adiga, Satish Kumar; Kalthur, Guruprasad.

In: Journal of Assisted Reproduction and Genetics, Vol. 36, No. 2, 15.02.2019, p. 255-266.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Supplementation of biotin to sperm preparation medium enhances fertilizing ability of spermatozoa and improves preimplantation embryo development

AU - Salian, Sujith Raj

AU - Nayak, Guruprasad

AU - Kumari, Sandhya

AU - Patel, Sandesh

AU - Gowda, Shruthi

AU - Shenoy, Yashaswini

AU - Sugunan, Sinoy

AU - G.K, Rajanikant

AU - Managuli, Renuka Suresh

AU - Mutalik, Srinivas

AU - Dahiya, Vandana

AU - Pal, Samanwita

AU - Adiga, Satish Kumar

AU - Kalthur, Guruprasad

PY - 2019/2/15

Y1 - 2019/2/15

N2 - Purpose: Motility of spermatozoa helps not only in planning the type of infertility treatment but also directly reflects the success rate in assisted reproductive technology (ART). Previously, biotin, a water-soluble vitamin, has been shown to increase the motility and longevity of cryopreserved human spermatozoa. The present study was designed to understand the molecular basis of the beneficial effects of presence of biotin in sperm wash medium on early embryo development. Methods: The effect biotin supplementation to sperm wash medium on the sperm parameters were assessed in swim-up fraction of normozoospermic and asthenozoospermic ejaculates collected from infertile men. Fertilization and early embryo development was studied using Swiss albino mice. Results: Even though both biotin and pentoxifylline (PTX) enhanced the motility of spermatozoa from normozoospermic and asthenozoospermic samples, biotin group exhibited higher in vitro survival. Using mouse model, we observed that presence of biotin or PTX in sperm wash medium improved the fertilization rate and blastocyst rate compared to control. Blastocysts from these groups had significantly higher total cell number (P < 0.01) and lower apoptotic index. In silico target prediction revealed that GTPase HRas (HRas), tyrosine-protein phosphatase nonreceptor type 1 (PTP1B), and glucokinase are the probable targets for biotin. Solution-state Nuclear Magnetic Resonance (NMR) studies confirmed that biotin interacts both with human HRas and PTP1B. Conclusion: Our results indicate that presence of biotin in sperm wash medium can improve the fertilization potential and preimplantation embryo development and can be considered as a safe alternate to PTX.

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