Surface Engineered Protein Nanoparticles with Hyaluronic Acid Based Multilayers for Targeted Delivery of Anticancer Agents

Sreeranjini Pulakkat, Sai A. Balaji, Annapoorni Rangarajan, Ashok M. Raichur

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Layer-by-layer (LbL) technique was employed to modify the surface of doxorubicin (Dox)-loaded bovine serum albumin (BSA) nanoparticles using hyaluronic acid (HA) to enable targeted delivery to overexpressed CD44 receptors in metastatic breast cancer cells. LbL technique offers a versatile approach to modify the surface of colloidal nanoparticles without any covalent modification. Dox-loaded BSA (Dox Ab) nanoparticles optimized for their size, zeta potential, and drug encapsulation efficiency were prepared by modified desolvation technique. The cellular uptake and cytotoxicity of the LbL coated Dox Ab nanoparticles were analyzed in CD44 overexpressing breast cancer cell line MDA-MB-231. Nanoparticles with HA as the final layer (Dox Ab HA) showed maximum cellular uptake in MDA-MB-231 cells owing to the CD44 receptor-mediated endocytosis and hence, exhibited more cytotoxicity as compared to free Dox. Further, luciferase-transfected MDA-MB-231 cells were used to induce tumor in BALB/c female nude mice to enable whole body tumor imaging. The mice were imaged before and after Dox treatment to visualize the tumor growth. The in vivo biodistribution of Dox Ab HA nanoparticles in nude mice showed maximum accumulation in tumor, and importantly, better tumor reduction in comparison with free Dox, thus paving the way for improved drug delivery into tumors.

Original languageEnglish
Pages (from-to)23437-23449
Number of pages13
JournalACS Applied Materials and Interfaces
Volume8
Issue number36
DOIs
Publication statusPublished - 14-09-2016
Externally publishedYes

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Hyaluronic acid
Hyaluronic Acid
Antineoplastic Agents
Doxorubicin
Tumors
Multilayers
Membrane Proteins
Nanoparticles
Proteins
Cytotoxicity
Bovine Serum Albumin
Cells
Zeta potential
Drug delivery
Encapsulation
Luciferases
Imaging techniques

All Science Journal Classification (ASJC) codes

  • Materials Science(all)

Cite this

Pulakkat, Sreeranjini ; Balaji, Sai A. ; Rangarajan, Annapoorni ; Raichur, Ashok M. / Surface Engineered Protein Nanoparticles with Hyaluronic Acid Based Multilayers for Targeted Delivery of Anticancer Agents. In: ACS Applied Materials and Interfaces. 2016 ; Vol. 8, No. 36. pp. 23437-23449.
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Surface Engineered Protein Nanoparticles with Hyaluronic Acid Based Multilayers for Targeted Delivery of Anticancer Agents. / Pulakkat, Sreeranjini; Balaji, Sai A.; Rangarajan, Annapoorni; Raichur, Ashok M.

In: ACS Applied Materials and Interfaces, Vol. 8, No. 36, 14.09.2016, p. 23437-23449.

Research output: Contribution to journalArticle

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