Susceptibility of peripheral lymphocytes of brain tumour patients to in vitro radiation-induced DNA damage, a preliminary study

Guruprasad Kalthur, Prem Kumar, Uma Devi, Sabir Ali, Ramya Upadhya, Sailaja Pillai, Anjali Rao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: The present investigation aimed to study the susceptibility of lymphocytes collected from brain tumour patients to radiation-induced DNA damage under in vitro conditions. Methods: The peripheral lymphocytes collected from brain tumour patients were exposed to 2-Gy gamma radiation. Susceptibility of lymphocytes to radiation-induced DNA damage and their repair ability was assessed by alkaline comet assay. Results: Lymphocytes of patients with benign and malignant tumour had a significantly higher (p < 0.001) baseline DNA damage compared to lymphocytes from normal subjects. A significant increase (p < 0.001) in DNA damage was observed immediately after irradiation of lymphocytes from healthy subjects and brain tumour patients. However, at 1 h after irradiation the level of DNA damage dropped significantly (p < 0.001) compared to that of immediately after irradiation of respective groups. Discussion: In conclusion, the lymphocytes of brain tumour patients possess a higher level of basal DNA damage and exhibit a higher susceptibility to a clastogenic agent like radiation.

Original languageEnglish
Pages (from-to)147-150
Number of pages4
JournalClinical and Experimental Medicine
Volume8
Issue number3
DOIs
Publication statusPublished - 09-2008

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Lymphocytes
Brain Neoplasms
DNA Damage
Tumors
Brain
Radiation
DNA
Irradiation
Radiation Dosage
Comet Assay
Gamma Rays
In Vitro Techniques
Gamma rays
Assays
Healthy Volunteers
Repair
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kalthur, Guruprasad ; Kumar, Prem ; Devi, Uma ; Ali, Sabir ; Upadhya, Ramya ; Pillai, Sailaja ; Rao, Anjali. / Susceptibility of peripheral lymphocytes of brain tumour patients to in vitro radiation-induced DNA damage, a preliminary study. In: Clinical and Experimental Medicine. 2008 ; Vol. 8, No. 3. pp. 147-150.
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Susceptibility of peripheral lymphocytes of brain tumour patients to in vitro radiation-induced DNA damage, a preliminary study. / Kalthur, Guruprasad; Kumar, Prem; Devi, Uma; Ali, Sabir; Upadhya, Ramya; Pillai, Sailaja; Rao, Anjali.

In: Clinical and Experimental Medicine, Vol. 8, No. 3, 09.2008, p. 147-150.

Research output: Contribution to journalArticle

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