Systemic Toxicity and Teratogenicity of Copper Oxide Nanoparticles and Copper Sulfate

Ambika Vishnu Kadammattil, Shyama Prasad Sajankila, Suma Prabhu, Bhuvanagiri Nageshwar Rao, B S Satish Rao

Research output: Contribution to journalArticle

Abstract

Acceleration in development of metallic nanoparticles for their utility in medical and technological applications due to their unique physicochemical properties has concurrently raised a matter of concern due to their potential toxicity. Of the enormous metallic nanostructures, copper oxide nanoparticles (CuONPs) having optical and electrochemical properties are scrutinized for theranostic applications. Therefore, their safety profile is of a major concern in optimizing a safe dose for its clinical utility. Considering the potency of CuONPs in epitomizing toxicity, we report a dose and time dependent acute, systemic and transgenerational toxicity profile of CuONPs in comparison to the bulk copper as copper sulfate (CuSO4). Acute toxic dose (LD50(14)) of CuONPs (400 mg/kg · b · wt) was found to be three fold higher that of CuSO4(100 mg/kg · b · wt). Comparative steady state evaluation showed that CuONPs (≥5 mg/kg · b · wt.) induce greater dose and time dependent oxidative stress by increase in protein carbonylation and decreased glutathione levels in comparison to the bulk CuSO4. Furthermore, CuONPs were found to disrupt blood brain barrier (BBB) and sneak in to the brain which was quantified by atomic absorption spectroscopy (AAS) and also coax toxicity in liver, kidney and spleen, ascertained by histopathological findings (at ≥5 mg/kg · b · wt.). Considering transgenerational toxicity, CuONPs in comparison to CuSO4 severely affected sperm count and morphology in male animals, though not much teratological effects were observed, except certain extent of embryo resorption. The present study highlights a complete toxicity profile of CuONPs, giving forethought for considering them for clinical applications.

Original languageEnglish
Pages (from-to)2394-2404
Number of pages11
JournalJournal of Nanoscience and Nanotechnology
Volume18
Issue number4
DOIs
Publication statusPublished - 01-04-2018

Fingerprint

Copper Sulfate
Copper oxides
copper oxides
toxicity
Nanoparticles
Oxides
Toxicity
Copper
sulfates
Embryo Loss
Protein Carbonylation
Metal Nanoparticles
copper
nanoparticles
Sperm Count
Nanostructures
Poisons
Lethal Dose 50
Blood-Brain Barrier
Glutathione

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Kadammattil, Ambika Vishnu ; Sajankila, Shyama Prasad ; Prabhu, Suma ; Rao, Bhuvanagiri Nageshwar ; Rao, B S Satish . / Systemic Toxicity and Teratogenicity of Copper Oxide Nanoparticles and Copper Sulfate. In: Journal of Nanoscience and Nanotechnology. 2018 ; Vol. 18, No. 4. pp. 2394-2404.
@article{f590e953ba004fcebf2f064b220f5762,
title = "Systemic Toxicity and Teratogenicity of Copper Oxide Nanoparticles and Copper Sulfate",
abstract = "Acceleration in development of metallic nanoparticles for their utility in medical and technological applications due to their unique physicochemical properties has concurrently raised a matter of concern due to their potential toxicity. Of the enormous metallic nanostructures, copper oxide nanoparticles (CuONPs) having optical and electrochemical properties are scrutinized for theranostic applications. Therefore, their safety profile is of a major concern in optimizing a safe dose for its clinical utility. Considering the potency of CuONPs in epitomizing toxicity, we report a dose and time dependent acute, systemic and transgenerational toxicity profile of CuONPs in comparison to the bulk copper as copper sulfate (CuSO4). Acute toxic dose (LD50(14)) of CuONPs (400 mg/kg · b · wt) was found to be three fold higher that of CuSO4(100 mg/kg · b · wt). Comparative steady state evaluation showed that CuONPs (≥5 mg/kg · b · wt.) induce greater dose and time dependent oxidative stress by increase in protein carbonylation and decreased glutathione levels in comparison to the bulk CuSO4. Furthermore, CuONPs were found to disrupt blood brain barrier (BBB) and sneak in to the brain which was quantified by atomic absorption spectroscopy (AAS) and also coax toxicity in liver, kidney and spleen, ascertained by histopathological findings (at ≥5 mg/kg · b · wt.). Considering transgenerational toxicity, CuONPs in comparison to CuSO4 severely affected sperm count and morphology in male animals, though not much teratological effects were observed, except certain extent of embryo resorption. The present study highlights a complete toxicity profile of CuONPs, giving forethought for considering them for clinical applications.",
author = "Kadammattil, {Ambika Vishnu} and Sajankila, {Shyama Prasad} and Suma Prabhu and Rao, {Bhuvanagiri Nageshwar} and Rao, {B S Satish}",
year = "2018",
month = "4",
day = "1",
doi = "10.1166/jnn.2018.14542",
language = "English",
volume = "18",
pages = "2394--2404",
journal = "Journal of Nanoscience and Nanotechnology",
issn = "1533-4880",
publisher = "American Scientific Publishers",
number = "4",

}

Systemic Toxicity and Teratogenicity of Copper Oxide Nanoparticles and Copper Sulfate. / Kadammattil, Ambika Vishnu; Sajankila, Shyama Prasad; Prabhu, Suma; Rao, Bhuvanagiri Nageshwar; Rao, B S Satish .

In: Journal of Nanoscience and Nanotechnology, Vol. 18, No. 4, 01.04.2018, p. 2394-2404.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Systemic Toxicity and Teratogenicity of Copper Oxide Nanoparticles and Copper Sulfate

AU - Kadammattil, Ambika Vishnu

AU - Sajankila, Shyama Prasad

AU - Prabhu, Suma

AU - Rao, Bhuvanagiri Nageshwar

AU - Rao, B S Satish

PY - 2018/4/1

Y1 - 2018/4/1

N2 - Acceleration in development of metallic nanoparticles for their utility in medical and technological applications due to their unique physicochemical properties has concurrently raised a matter of concern due to their potential toxicity. Of the enormous metallic nanostructures, copper oxide nanoparticles (CuONPs) having optical and electrochemical properties are scrutinized for theranostic applications. Therefore, their safety profile is of a major concern in optimizing a safe dose for its clinical utility. Considering the potency of CuONPs in epitomizing toxicity, we report a dose and time dependent acute, systemic and transgenerational toxicity profile of CuONPs in comparison to the bulk copper as copper sulfate (CuSO4). Acute toxic dose (LD50(14)) of CuONPs (400 mg/kg · b · wt) was found to be three fold higher that of CuSO4(100 mg/kg · b · wt). Comparative steady state evaluation showed that CuONPs (≥5 mg/kg · b · wt.) induce greater dose and time dependent oxidative stress by increase in protein carbonylation and decreased glutathione levels in comparison to the bulk CuSO4. Furthermore, CuONPs were found to disrupt blood brain barrier (BBB) and sneak in to the brain which was quantified by atomic absorption spectroscopy (AAS) and also coax toxicity in liver, kidney and spleen, ascertained by histopathological findings (at ≥5 mg/kg · b · wt.). Considering transgenerational toxicity, CuONPs in comparison to CuSO4 severely affected sperm count and morphology in male animals, though not much teratological effects were observed, except certain extent of embryo resorption. The present study highlights a complete toxicity profile of CuONPs, giving forethought for considering them for clinical applications.

AB - Acceleration in development of metallic nanoparticles for their utility in medical and technological applications due to their unique physicochemical properties has concurrently raised a matter of concern due to their potential toxicity. Of the enormous metallic nanostructures, copper oxide nanoparticles (CuONPs) having optical and electrochemical properties are scrutinized for theranostic applications. Therefore, their safety profile is of a major concern in optimizing a safe dose for its clinical utility. Considering the potency of CuONPs in epitomizing toxicity, we report a dose and time dependent acute, systemic and transgenerational toxicity profile of CuONPs in comparison to the bulk copper as copper sulfate (CuSO4). Acute toxic dose (LD50(14)) of CuONPs (400 mg/kg · b · wt) was found to be three fold higher that of CuSO4(100 mg/kg · b · wt). Comparative steady state evaluation showed that CuONPs (≥5 mg/kg · b · wt.) induce greater dose and time dependent oxidative stress by increase in protein carbonylation and decreased glutathione levels in comparison to the bulk CuSO4. Furthermore, CuONPs were found to disrupt blood brain barrier (BBB) and sneak in to the brain which was quantified by atomic absorption spectroscopy (AAS) and also coax toxicity in liver, kidney and spleen, ascertained by histopathological findings (at ≥5 mg/kg · b · wt.). Considering transgenerational toxicity, CuONPs in comparison to CuSO4 severely affected sperm count and morphology in male animals, though not much teratological effects were observed, except certain extent of embryo resorption. The present study highlights a complete toxicity profile of CuONPs, giving forethought for considering them for clinical applications.

UR - http://www.scopus.com/inward/record.url?scp=85049892105&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85049892105&partnerID=8YFLogxK

U2 - 10.1166/jnn.2018.14542

DO - 10.1166/jnn.2018.14542

M3 - Article

VL - 18

SP - 2394

EP - 2404

JO - Journal of Nanoscience and Nanotechnology

JF - Journal of Nanoscience and Nanotechnology

SN - 1533-4880

IS - 4

ER -