Temperature sensitive liposomes of plumbagin: Characterization and in vivo evaluation in mice bearing melanoma B16F1

Sandip B. Tiwari, Raveendra M. Pai, N. Udupa

Research output: Contribution to journalReview article

26 Citations (Scopus)

Abstract

The effectiveness of the combination of thermosensitive liposomes of plumbagin and hyperthermia is described. Small-sized, thermosensitive liposomes were prepared by thin film hydration and subsequent sonication. The liposomes were characterized for size, phase transition temperature, in vitro drug release and stability. The results of particle size analysis indicated that almost 90% of the vesicles were below 0.19 μm size. The phase transition temperature of the liposomes as determined by differential scanning calorimetry was found to be 41.32°C. The results of in vitro release studies in phosphate buffered saline + mouse plasma indicated that maximum drug release (51.25%) occurred at 42°C compared to the less than 9% release at 37°C. Better stability profile was observed when the plumbagin liposomes were stored at 4°C. When combined with localised hyperthermia (43°C, 30 min or 1 h), liposomal plumbagin administered intravenously to C57BL/6J mice bearing melanoma exhibited better anticancer activity as compared to animals treated with an equivalent dose of free plumbagin with or without hyperthermia, which was evident by enhanced volume doubling time and growth delay.

Original languageEnglish
Pages (from-to)585-591
Number of pages7
JournalJournal of Drug Targeting
Volume10
Issue number8
DOIs
Publication statusPublished - 12-2002

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Liposomes
Melanoma
Temperature
Fever
Transition Temperature
Phase Transition
Drug Stability
Sonication
Differential Scanning Calorimetry
Inbred C57BL Mouse
Particle Size
Phosphates
plumbagin
Growth
In Vitro Techniques
Drug Liberation

All Science Journal Classification (ASJC) codes

  • Pharmaceutical Science

Cite this

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title = "Temperature sensitive liposomes of plumbagin: Characterization and in vivo evaluation in mice bearing melanoma B16F1",
abstract = "The effectiveness of the combination of thermosensitive liposomes of plumbagin and hyperthermia is described. Small-sized, thermosensitive liposomes were prepared by thin film hydration and subsequent sonication. The liposomes were characterized for size, phase transition temperature, in vitro drug release and stability. The results of particle size analysis indicated that almost 90{\%} of the vesicles were below 0.19 μm size. The phase transition temperature of the liposomes as determined by differential scanning calorimetry was found to be 41.32°C. The results of in vitro release studies in phosphate buffered saline + mouse plasma indicated that maximum drug release (51.25{\%}) occurred at 42°C compared to the less than 9{\%} release at 37°C. Better stability profile was observed when the plumbagin liposomes were stored at 4°C. When combined with localised hyperthermia (43°C, 30 min or 1 h), liposomal plumbagin administered intravenously to C57BL/6J mice bearing melanoma exhibited better anticancer activity as compared to animals treated with an equivalent dose of free plumbagin with or without hyperthermia, which was evident by enhanced volume doubling time and growth delay.",
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Temperature sensitive liposomes of plumbagin : Characterization and in vivo evaluation in mice bearing melanoma B16F1. / Tiwari, Sandip B.; Pai, Raveendra M.; Udupa, N.

In: Journal of Drug Targeting, Vol. 10, No. 8, 12.2002, p. 585-591.

Research output: Contribution to journalReview article

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N2 - The effectiveness of the combination of thermosensitive liposomes of plumbagin and hyperthermia is described. Small-sized, thermosensitive liposomes were prepared by thin film hydration and subsequent sonication. The liposomes were characterized for size, phase transition temperature, in vitro drug release and stability. The results of particle size analysis indicated that almost 90% of the vesicles were below 0.19 μm size. The phase transition temperature of the liposomes as determined by differential scanning calorimetry was found to be 41.32°C. The results of in vitro release studies in phosphate buffered saline + mouse plasma indicated that maximum drug release (51.25%) occurred at 42°C compared to the less than 9% release at 37°C. Better stability profile was observed when the plumbagin liposomes were stored at 4°C. When combined with localised hyperthermia (43°C, 30 min or 1 h), liposomal plumbagin administered intravenously to C57BL/6J mice bearing melanoma exhibited better anticancer activity as compared to animals treated with an equivalent dose of free plumbagin with or without hyperthermia, which was evident by enhanced volume doubling time and growth delay.

AB - The effectiveness of the combination of thermosensitive liposomes of plumbagin and hyperthermia is described. Small-sized, thermosensitive liposomes were prepared by thin film hydration and subsequent sonication. The liposomes were characterized for size, phase transition temperature, in vitro drug release and stability. The results of particle size analysis indicated that almost 90% of the vesicles were below 0.19 μm size. The phase transition temperature of the liposomes as determined by differential scanning calorimetry was found to be 41.32°C. The results of in vitro release studies in phosphate buffered saline + mouse plasma indicated that maximum drug release (51.25%) occurred at 42°C compared to the less than 9% release at 37°C. Better stability profile was observed when the plumbagin liposomes were stored at 4°C. When combined with localised hyperthermia (43°C, 30 min or 1 h), liposomal plumbagin administered intravenously to C57BL/6J mice bearing melanoma exhibited better anticancer activity as compared to animals treated with an equivalent dose of free plumbagin with or without hyperthermia, which was evident by enhanced volume doubling time and growth delay.

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