The antibiotic susceptibility patterns of uropathogenic escherichia coli, with special reference to the fluoroquinolones

V. A. Abdul Rahaman Shariff, Shenoy M. Suchitra, Taruna Yadav, M. Radhakrishna

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Context: The emergence of drug resistance to trimethoprimsulfamethoxazole, the penicillins, ephalosporins, and fluoroquinolones by Uropathogenic Escherichia coli (UPEC) has limited the options for selecting the appropriate antibiotic for the treatment of urinary tract infections. Aims: The The E. coli isolates, which were obtained from the culture of urine samples,were studied for their antibiotic resistance patterns, with special reference to the antimicrobial activity of the fluoroquinolones and the production of the extended spectrum β-lactamases. (ESBL), Settings and Design: This was a hospital based, prospective study which was done for a period of eighteen months. Methods and Material: This study was done by using the standard culture techniques for urine samples, the modified Kirby-Bauer disk diffusion method for the antibiotic susceptibility testing and the disk diffusion method to confirm the ESBL production by the clinical isolates of E. coli in urine. The sensitivity pattern was correlated with the clinical condition and the presence of the risk factors. The statistical analysis which was used: The statistical analysis was done by using the proportions of sensitive, resistant and intermediates. Descriptive statistics like the total, mean and percentage were done by using the Statistical Package for the Social Sciences (SPSS), version 15.0. Results: The hospital isolates showed high degrees of resistance to the penicillins, cephalosporins, nalidixic acid and the fluoroquinolones, with 59% of the isolates being ESBL producers. This study also revealed that the isolates which were resistant to nalidixic acid had a higher MIC for ciprofloxacin as compared to the strains which were sensitive. Conclusions: The incidence of the multidrug resistant strains of Escherichia coli has been steadily increasing over the past few years. The knowledge on the resistance pattern of the bacterial strains in a geographical area will help in guiding the appropriate and the judicious use of antibiotics. Also, the formulation of an appropriate hospital antibiotic policy will go a long way in controlling these infections.

Original languageEnglish
Pages (from-to)1027-1030
Number of pages4
JournalJournal of Clinical and Diagnostic Research
Volume7
Issue number6
DOIs
Publication statusPublished - 17-06-2013

Fingerprint

Uropathogenic Escherichia coli
Fluoroquinolones
Escherichia coli
Anti-Bacterial Agents
Nalidixic Acid
Urine
Disk Diffusion Antimicrobial Tests
Penicillins
Penicillin Resistance
Statistical methods
Culture Techniques
Social Sciences
Cephalosporins
Ciprofloxacin
Microbial Drug Resistance
Drug Resistance
Urinary Tract Infections
Social sciences
Prospective Studies
Statistics

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Clinical Biochemistry

Cite this

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title = "The antibiotic susceptibility patterns of uropathogenic escherichia coli, with special reference to the fluoroquinolones",
abstract = "Context: The emergence of drug resistance to trimethoprimsulfamethoxazole, the penicillins, ephalosporins, and fluoroquinolones by Uropathogenic Escherichia coli (UPEC) has limited the options for selecting the appropriate antibiotic for the treatment of urinary tract infections. Aims: The The E. coli isolates, which were obtained from the culture of urine samples,were studied for their antibiotic resistance patterns, with special reference to the antimicrobial activity of the fluoroquinolones and the production of the extended spectrum β-lactamases. (ESBL), Settings and Design: This was a hospital based, prospective study which was done for a period of eighteen months. Methods and Material: This study was done by using the standard culture techniques for urine samples, the modified Kirby-Bauer disk diffusion method for the antibiotic susceptibility testing and the disk diffusion method to confirm the ESBL production by the clinical isolates of E. coli in urine. The sensitivity pattern was correlated with the clinical condition and the presence of the risk factors. The statistical analysis which was used: The statistical analysis was done by using the proportions of sensitive, resistant and intermediates. Descriptive statistics like the total, mean and percentage were done by using the Statistical Package for the Social Sciences (SPSS), version 15.0. Results: The hospital isolates showed high degrees of resistance to the penicillins, cephalosporins, nalidixic acid and the fluoroquinolones, with 59{\%} of the isolates being ESBL producers. This study also revealed that the isolates which were resistant to nalidixic acid had a higher MIC for ciprofloxacin as compared to the strains which were sensitive. Conclusions: The incidence of the multidrug resistant strains of Escherichia coli has been steadily increasing over the past few years. The knowledge on the resistance pattern of the bacterial strains in a geographical area will help in guiding the appropriate and the judicious use of antibiotics. Also, the formulation of an appropriate hospital antibiotic policy will go a long way in controlling these infections.",
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The antibiotic susceptibility patterns of uropathogenic escherichia coli, with special reference to the fluoroquinolones. / Abdul Rahaman Shariff, V. A.; Suchitra, Shenoy M.; Yadav, Taruna; Radhakrishna, M.

In: Journal of Clinical and Diagnostic Research, Vol. 7, No. 6, 17.06.2013, p. 1027-1030.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The antibiotic susceptibility patterns of uropathogenic escherichia coli, with special reference to the fluoroquinolones

AU - Abdul Rahaman Shariff, V. A.

AU - Suchitra, Shenoy M.

AU - Yadav, Taruna

AU - Radhakrishna, M.

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N2 - Context: The emergence of drug resistance to trimethoprimsulfamethoxazole, the penicillins, ephalosporins, and fluoroquinolones by Uropathogenic Escherichia coli (UPEC) has limited the options for selecting the appropriate antibiotic for the treatment of urinary tract infections. Aims: The The E. coli isolates, which were obtained from the culture of urine samples,were studied for their antibiotic resistance patterns, with special reference to the antimicrobial activity of the fluoroquinolones and the production of the extended spectrum β-lactamases. (ESBL), Settings and Design: This was a hospital based, prospective study which was done for a period of eighteen months. Methods and Material: This study was done by using the standard culture techniques for urine samples, the modified Kirby-Bauer disk diffusion method for the antibiotic susceptibility testing and the disk diffusion method to confirm the ESBL production by the clinical isolates of E. coli in urine. The sensitivity pattern was correlated with the clinical condition and the presence of the risk factors. The statistical analysis which was used: The statistical analysis was done by using the proportions of sensitive, resistant and intermediates. Descriptive statistics like the total, mean and percentage were done by using the Statistical Package for the Social Sciences (SPSS), version 15.0. Results: The hospital isolates showed high degrees of resistance to the penicillins, cephalosporins, nalidixic acid and the fluoroquinolones, with 59% of the isolates being ESBL producers. This study also revealed that the isolates which were resistant to nalidixic acid had a higher MIC for ciprofloxacin as compared to the strains which were sensitive. Conclusions: The incidence of the multidrug resistant strains of Escherichia coli has been steadily increasing over the past few years. The knowledge on the resistance pattern of the bacterial strains in a geographical area will help in guiding the appropriate and the judicious use of antibiotics. Also, the formulation of an appropriate hospital antibiotic policy will go a long way in controlling these infections.

AB - Context: The emergence of drug resistance to trimethoprimsulfamethoxazole, the penicillins, ephalosporins, and fluoroquinolones by Uropathogenic Escherichia coli (UPEC) has limited the options for selecting the appropriate antibiotic for the treatment of urinary tract infections. Aims: The The E. coli isolates, which were obtained from the culture of urine samples,were studied for their antibiotic resistance patterns, with special reference to the antimicrobial activity of the fluoroquinolones and the production of the extended spectrum β-lactamases. (ESBL), Settings and Design: This was a hospital based, prospective study which was done for a period of eighteen months. Methods and Material: This study was done by using the standard culture techniques for urine samples, the modified Kirby-Bauer disk diffusion method for the antibiotic susceptibility testing and the disk diffusion method to confirm the ESBL production by the clinical isolates of E. coli in urine. The sensitivity pattern was correlated with the clinical condition and the presence of the risk factors. The statistical analysis which was used: The statistical analysis was done by using the proportions of sensitive, resistant and intermediates. Descriptive statistics like the total, mean and percentage were done by using the Statistical Package for the Social Sciences (SPSS), version 15.0. Results: The hospital isolates showed high degrees of resistance to the penicillins, cephalosporins, nalidixic acid and the fluoroquinolones, with 59% of the isolates being ESBL producers. This study also revealed that the isolates which were resistant to nalidixic acid had a higher MIC for ciprofloxacin as compared to the strains which were sensitive. Conclusions: The incidence of the multidrug resistant strains of Escherichia coli has been steadily increasing over the past few years. The knowledge on the resistance pattern of the bacterial strains in a geographical area will help in guiding the appropriate and the judicious use of antibiotics. Also, the formulation of an appropriate hospital antibiotic policy will go a long way in controlling these infections.

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