The ArsD as(III) metallochaperone

A. Abdul Ajees, Jianbo Yang, Barry P. Rosen

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Arsenic, a toxic metalloid widely existing in the environment, causes a variety of health problems. The ars operon encoded by Escherichia coli plasmid R773 has arsD and arsA genes, where ArsA is an ATPase that is the catalytic subunit of the ArsAB As(III) extrusion pump, and ArsD is an arsenic chaperone for ArsA. ArsD transfers As(III) to ArsA and increases the affinity of ArsA for As(III), allowing resistance to environmental concentrations of arsenic. Cys12, Cys13 and Cys18 in ArsD form a three sulfur-coordinated As(III) binding site that is essential for metallochaperone activity. ATP hydrolysis by ArsA is required for transfer of As(III) from ArsD to ArsA, suggesting that transfer occurs with a conformation of ArsA that transiently forms during the catalytic cycle. The 1.4 Å; x-ray crystal structure of ArsD shows a core of four β-strands flanked by four α-helices in a thioredoxin fold. Docking of ArsD with ArsA was modeled in silico. Independently ArsD mutants exhibiting either weaker or stronger interaction with ArsA were selected. The locations of the mutations mapped on the surface of ArsD are consistent with the docking model. The results suggest that the interface with ArsA involves one surface of α1 helix and metalloid binding site of ArsD.

Original languageEnglish
Pages (from-to)391-399
Number of pages9
JournalBioMetals
Volume24
Issue number3
DOIs
Publication statusPublished - 01-06-2011

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Metallochaperones
Arsenic
arsenic
Metalloids
Binding sites
binding sites
Binding Sites
Thioredoxins
Poisons
Adenosinetriphosphate
protein subunits
operon
Operon
Medical problems
crystal structure
Sulfur
extrusion
pumps
Computer Simulation
Escherichia coli

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Metals and Alloys
  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ajees, A. Abdul ; Yang, Jianbo ; Rosen, Barry P. / The ArsD as(III) metallochaperone. In: BioMetals. 2011 ; Vol. 24, No. 3. pp. 391-399.
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Ajees, AA, Yang, J & Rosen, BP 2011, 'The ArsD as(III) metallochaperone', BioMetals, vol. 24, no. 3, pp. 391-399. https://doi.org/10.1007/s10534-010-9398-x

The ArsD as(III) metallochaperone. / Ajees, A. Abdul; Yang, Jianbo; Rosen, Barry P.

In: BioMetals, Vol. 24, No. 3, 01.06.2011, p. 391-399.

Research output: Contribution to journalArticle

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