The effect of ethanol extract of Wrightia tinctoria bark on wound healing in rats

V.P. Veerapur, M.B. Palkar, H. Srinivasa, M.S. Kumar, S. Patra, P.G.M. Rao, K.K. Srinivasan

Research output: Contribution to journalArticle

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Abstract

Objectives: To screen the wound healing activity of ethanol extract of Wrightia tinctoria bark using incision, excision and dead space wound models and evaluate histopathological and biochemical changes of granuloma tissue. Material and method: The bark powder of W. tinctoria was extracted with 95% ethanol by continuous heat extraction and was subjected for phytochemical investigation and screened for wound healing activity in the incision, excision and dead space wound models in rats. A supportive study made on granuloma tissue to estimate the hydroxyproline content and histopathological examination to determine the pattern of lay-down for collagen using Masson Trichrome stain. Results: Triterpenoids, steroids and saponins were found to be present in ethanol extract of the barks of W. tinctoria. In resutured incision wound model, ethanol extract showed significant breaking strength (P
Original languageEnglish
Pages (from-to)155-159
Number of pages5
JournalJournal of Natural Remedies
Volume4
Issue number2
Publication statusPublished - 2004

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Wound Healing
Ethanol
Space Simulation
Granuloma
Wounds and Injuries
Hydroxyproline
Saponins
Phytochemicals
Powders
Collagen
Hot Temperature
Steroids

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Veerapur, V. P., Palkar, M. B., Srinivasa, H., Kumar, M. S., Patra, S., Rao, P. G. M., & Srinivasan, K. K. (2004). The effect of ethanol extract of Wrightia tinctoria bark on wound healing in rats. Journal of Natural Remedies, 4(2), 155-159.
Veerapur, V.P. ; Palkar, M.B. ; Srinivasa, H. ; Kumar, M.S. ; Patra, S. ; Rao, P.G.M. ; Srinivasan, K.K. / The effect of ethanol extract of Wrightia tinctoria bark on wound healing in rats. In: Journal of Natural Remedies. 2004 ; Vol. 4, No. 2. pp. 155-159.
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abstract = "Objectives: To screen the wound healing activity of ethanol extract of Wrightia tinctoria bark using incision, excision and dead space wound models and evaluate histopathological and biochemical changes of granuloma tissue. Material and method: The bark powder of W. tinctoria was extracted with 95{\%} ethanol by continuous heat extraction and was subjected for phytochemical investigation and screened for wound healing activity in the incision, excision and dead space wound models in rats. A supportive study made on granuloma tissue to estimate the hydroxyproline content and histopathological examination to determine the pattern of lay-down for collagen using Masson Trichrome stain. Results: Triterpenoids, steroids and saponins were found to be present in ethanol extract of the barks of W. tinctoria. In resutured incision wound model, ethanol extract showed significant breaking strength (P",
author = "V.P. Veerapur and M.B. Palkar and H. Srinivasa and M.S. Kumar and S. Patra and P.G.M. Rao and K.K. Srinivasan",
note = "Cited By :25 Export Date: 10 November 2017 CODEN: JNROA Correspondence Address: Veerapur, V.P.; Dept. of Pharmaceutical Chemistry, Manipal Coll. of Pharmaceut. Sci., Manipal-576 119 Karnataka, India; email: veeresh36@yahoo.com Chemicals/CAS: alcohol, 64-17-5; alpha amyrin, 638-95-9; beta amyrin, 559-70-6; collagen, 9007-34-5; gum tragacanth, 9000-65-1; hydroxyproline, 51-35-4, 6912-67-0; saponin, 8047-15-2 References: Warrier, P.K., Nambiar, V.P.K., Ramankutty, C., (1996) Indian Medicinal Plants, A Compendium of 500 Species, 5, pp. 417-419. , Vaidyanathum PS. (Eds.) Varier's Orient Longman Ltd: Madras; Nadkarni, K.M., (1982) Indian Materia Medica, 1, pp. 635-637. , Popular Prakashan: Bombay; Ramchandra, P., (1993) J. Natural Products, 56 (10), pp. 1811-1812; Rangaswami, S., Mangeswara Rao, M., (1963) Proc. Indian Acad. Sci., 57 A, pp. 115-120; Nesamony, S., (1993) Oushadha Sasyangal, pp. 175-176. , State Institute of Languages: Kerala; Harborne, J.B., (1998) Phytochemical Methods, pp. 60-66. , Chapman and Hall: London; Kaokate, C.K., (1991) Practical Pharmacognosy, pp. 107-112. , Vallabh Prakashan: Delhi; Ghosh, M.N., (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , 2nd Edn. Scientific book Agency: Culcutta; Ehrlich, H.P., Hunt, T.K., (1969) Ann. Surg., 170, pp. 203-206; Lee, K.H., (1968) J. Pharm. Sci., 57, pp. 1042-1047; Morton, J.J.P., Malone, M.H., (1972) Arch. Int. Pharmacodyn., 196, pp. 117-136; Patil, P.A., Kulkarni, D.R., (1984) Indian. J. Med. Res., 79, pp. 445-447; Schilling, A., (1968) J. Physiol. Rev., 48, pp. 374-375; Neuman, R.E., Logan, M.A., (1950) J. Biol. Chem., 186, pp. 549-552; Kulkarni, S.K., (1993) Hand Book of Experimental Pharmacology, pp. 85-96. , Vallabh Prakashan: Delhi; Tenni, R., (1988) Ital. J. Biochem., 37, pp. 69-77; Sunitha, S., Nagaraj, M., Varalakshmi, P., (2001) Fitoterapia, 72, pp. 516-523; Shobha, S.N., Gurumadhava Rao, S., (1999) Indian J. Physiol. Pharmacol., 43, pp. 230-234",
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Veerapur, VP, Palkar, MB, Srinivasa, H, Kumar, MS, Patra, S, Rao, PGM & Srinivasan, KK 2004, 'The effect of ethanol extract of Wrightia tinctoria bark on wound healing in rats', Journal of Natural Remedies, vol. 4, no. 2, pp. 155-159.

The effect of ethanol extract of Wrightia tinctoria bark on wound healing in rats. / Veerapur, V.P.; Palkar, M.B.; Srinivasa, H.; Kumar, M.S.; Patra, S.; Rao, P.G.M.; Srinivasan, K.K.

In: Journal of Natural Remedies, Vol. 4, No. 2, 2004, p. 155-159.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of ethanol extract of Wrightia tinctoria bark on wound healing in rats

AU - Veerapur, V.P.

AU - Palkar, M.B.

AU - Srinivasa, H.

AU - Kumar, M.S.

AU - Patra, S.

AU - Rao, P.G.M.

AU - Srinivasan, K.K.

N1 - Cited By :25 Export Date: 10 November 2017 CODEN: JNROA Correspondence Address: Veerapur, V.P.; Dept. of Pharmaceutical Chemistry, Manipal Coll. of Pharmaceut. Sci., Manipal-576 119 Karnataka, India; email: veeresh36@yahoo.com Chemicals/CAS: alcohol, 64-17-5; alpha amyrin, 638-95-9; beta amyrin, 559-70-6; collagen, 9007-34-5; gum tragacanth, 9000-65-1; hydroxyproline, 51-35-4, 6912-67-0; saponin, 8047-15-2 References: Warrier, P.K., Nambiar, V.P.K., Ramankutty, C., (1996) Indian Medicinal Plants, A Compendium of 500 Species, 5, pp. 417-419. , Vaidyanathum PS. (Eds.) Varier's Orient Longman Ltd: Madras; Nadkarni, K.M., (1982) Indian Materia Medica, 1, pp. 635-637. , Popular Prakashan: Bombay; Ramchandra, P., (1993) J. Natural Products, 56 (10), pp. 1811-1812; Rangaswami, S., Mangeswara Rao, M., (1963) Proc. Indian Acad. Sci., 57 A, pp. 115-120; Nesamony, S., (1993) Oushadha Sasyangal, pp. 175-176. , State Institute of Languages: Kerala; Harborne, J.B., (1998) Phytochemical Methods, pp. 60-66. , Chapman and Hall: London; Kaokate, C.K., (1991) Practical Pharmacognosy, pp. 107-112. , Vallabh Prakashan: Delhi; Ghosh, M.N., (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , 2nd Edn. Scientific book Agency: Culcutta; Ehrlich, H.P., Hunt, T.K., (1969) Ann. Surg., 170, pp. 203-206; Lee, K.H., (1968) J. Pharm. Sci., 57, pp. 1042-1047; Morton, J.J.P., Malone, M.H., (1972) Arch. Int. Pharmacodyn., 196, pp. 117-136; Patil, P.A., Kulkarni, D.R., (1984) Indian. J. Med. Res., 79, pp. 445-447; Schilling, A., (1968) J. Physiol. Rev., 48, pp. 374-375; Neuman, R.E., Logan, M.A., (1950) J. Biol. Chem., 186, pp. 549-552; Kulkarni, S.K., (1993) Hand Book of Experimental Pharmacology, pp. 85-96. , Vallabh Prakashan: Delhi; Tenni, R., (1988) Ital. J. Biochem., 37, pp. 69-77; Sunitha, S., Nagaraj, M., Varalakshmi, P., (2001) Fitoterapia, 72, pp. 516-523; Shobha, S.N., Gurumadhava Rao, S., (1999) Indian J. Physiol. Pharmacol., 43, pp. 230-234

PY - 2004

Y1 - 2004

N2 - Objectives: To screen the wound healing activity of ethanol extract of Wrightia tinctoria bark using incision, excision and dead space wound models and evaluate histopathological and biochemical changes of granuloma tissue. Material and method: The bark powder of W. tinctoria was extracted with 95% ethanol by continuous heat extraction and was subjected for phytochemical investigation and screened for wound healing activity in the incision, excision and dead space wound models in rats. A supportive study made on granuloma tissue to estimate the hydroxyproline content and histopathological examination to determine the pattern of lay-down for collagen using Masson Trichrome stain. Results: Triterpenoids, steroids and saponins were found to be present in ethanol extract of the barks of W. tinctoria. In resutured incision wound model, ethanol extract showed significant breaking strength (P

AB - Objectives: To screen the wound healing activity of ethanol extract of Wrightia tinctoria bark using incision, excision and dead space wound models and evaluate histopathological and biochemical changes of granuloma tissue. Material and method: The bark powder of W. tinctoria was extracted with 95% ethanol by continuous heat extraction and was subjected for phytochemical investigation and screened for wound healing activity in the incision, excision and dead space wound models in rats. A supportive study made on granuloma tissue to estimate the hydroxyproline content and histopathological examination to determine the pattern of lay-down for collagen using Masson Trichrome stain. Results: Triterpenoids, steroids and saponins were found to be present in ethanol extract of the barks of W. tinctoria. In resutured incision wound model, ethanol extract showed significant breaking strength (P

M3 - Article

VL - 4

SP - 155

EP - 159

JO - Journal of Natural Remedies

JF - Journal of Natural Remedies

SN - 0972-5547

IS - 2

ER -

Veerapur VP, Palkar MB, Srinivasa H, Kumar MS, Patra S, Rao PGM et al. The effect of ethanol extract of Wrightia tinctoria bark on wound healing in rats. Journal of Natural Remedies. 2004;4(2):155-159.