The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells

Ganesh Chandra Jagetia, Manjeshwar Shrinath Baliga

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In order to evaluate the seasonal variation as well as cytotoxicity of different fractions of Alstonia scholaris R. Br. (ASE), the HeLa cells were treated with different doses of various fractions of ASE collected in monsoon, winter and summer. The exposure of HeLa cells to different extracts prepared from the stem bark collected in monsoon, winter and summer seasons resulted in a dose dependent increase in the cell killing effect of ASE and the highest cell killing effect was observed for the extract prepared from the summer collections. Similarly, treatment of HeLa cells with different doses of various fractions of the Alstonia scholaris extract viz. residue (ASERS), steroidal (ASEST), chloroform (ASECH), petroleum ether (ASEPE), diethyl ether (ASEDE), ethyl acetate (ASEEA), n-butanol (ASENB), aqueous (ASEAQ) and echitamine chloride (ECL) also resulted in a dose dependent decline in the cell viability, where the greatest cytotoxic effect was observed for residue (ASERS), followed by the whole extract (ASE) and chloroform (ASECH) fraction, while the least activity was observed for the steroidal (ASEST) fraction. The cytotoxicity declined ASERS > ASE > ASECH >ECL > ASEEA > ASEDE > ASEPE > ASENB > ASEAQ > ASEST in order. Our study demonstrates that the extract prepared from the summer collection, and the fractions containing the alkaloids were highly effective in cell killing. The extract of ASE was more powerful than the active principle echitamine present in ASE.

Original languageEnglish
Pages (from-to)37-42
Number of pages6
JournalJournal of Ethnopharmacology
Volume96
Issue number1-2
DOIs
Publication statusPublished - 04-01-2005
Externally publishedYes

Fingerprint

Alstonia
Chloroform
HeLa Cells
Antineoplastic Agents
1-Butanol
Ether
Chlorides
Alkaloids
Cell Survival
echitamine
ethyl acetate
naphtha

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Jagetia, Ganesh Chandra ; Baliga, Manjeshwar Shrinath. / The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells. In: Journal of Ethnopharmacology. 2005 ; Vol. 96, No. 1-2. pp. 37-42.
@article{e1ea242bc57948cda27e286dcee3bad4,
title = "The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells",
abstract = "In order to evaluate the seasonal variation as well as cytotoxicity of different fractions of Alstonia scholaris R. Br. (ASE), the HeLa cells were treated with different doses of various fractions of ASE collected in monsoon, winter and summer. The exposure of HeLa cells to different extracts prepared from the stem bark collected in monsoon, winter and summer seasons resulted in a dose dependent increase in the cell killing effect of ASE and the highest cell killing effect was observed for the extract prepared from the summer collections. Similarly, treatment of HeLa cells with different doses of various fractions of the Alstonia scholaris extract viz. residue (ASERS), steroidal (ASEST), chloroform (ASECH), petroleum ether (ASEPE), diethyl ether (ASEDE), ethyl acetate (ASEEA), n-butanol (ASENB), aqueous (ASEAQ) and echitamine chloride (ECL) also resulted in a dose dependent decline in the cell viability, where the greatest cytotoxic effect was observed for residue (ASERS), followed by the whole extract (ASE) and chloroform (ASECH) fraction, while the least activity was observed for the steroidal (ASEST) fraction. The cytotoxicity declined ASERS > ASE > ASECH >ECL > ASEEA > ASEDE > ASEPE > ASENB > ASEAQ > ASEST in order. Our study demonstrates that the extract prepared from the summer collection, and the fractions containing the alkaloids were highly effective in cell killing. The extract of ASE was more powerful than the active principle echitamine present in ASE.",
author = "Jagetia, {Ganesh Chandra} and Baliga, {Manjeshwar Shrinath}",
year = "2005",
month = "1",
day = "4",
doi = "10.1016/j.jep.2004.07.024",
language = "English",
volume = "96",
pages = "37--42",
journal = "Journal of Ethnopharmacology",
issn = "0378-8741",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells. / Jagetia, Ganesh Chandra; Baliga, Manjeshwar Shrinath.

In: Journal of Ethnopharmacology, Vol. 96, No. 1-2, 04.01.2005, p. 37-42.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The effect of seasonal variation on the antineoplastic activity of Alstonia scholaris R. Br. in HeLa cells

AU - Jagetia, Ganesh Chandra

AU - Baliga, Manjeshwar Shrinath

PY - 2005/1/4

Y1 - 2005/1/4

N2 - In order to evaluate the seasonal variation as well as cytotoxicity of different fractions of Alstonia scholaris R. Br. (ASE), the HeLa cells were treated with different doses of various fractions of ASE collected in monsoon, winter and summer. The exposure of HeLa cells to different extracts prepared from the stem bark collected in monsoon, winter and summer seasons resulted in a dose dependent increase in the cell killing effect of ASE and the highest cell killing effect was observed for the extract prepared from the summer collections. Similarly, treatment of HeLa cells with different doses of various fractions of the Alstonia scholaris extract viz. residue (ASERS), steroidal (ASEST), chloroform (ASECH), petroleum ether (ASEPE), diethyl ether (ASEDE), ethyl acetate (ASEEA), n-butanol (ASENB), aqueous (ASEAQ) and echitamine chloride (ECL) also resulted in a dose dependent decline in the cell viability, where the greatest cytotoxic effect was observed for residue (ASERS), followed by the whole extract (ASE) and chloroform (ASECH) fraction, while the least activity was observed for the steroidal (ASEST) fraction. The cytotoxicity declined ASERS > ASE > ASECH >ECL > ASEEA > ASEDE > ASEPE > ASENB > ASEAQ > ASEST in order. Our study demonstrates that the extract prepared from the summer collection, and the fractions containing the alkaloids were highly effective in cell killing. The extract of ASE was more powerful than the active principle echitamine present in ASE.

AB - In order to evaluate the seasonal variation as well as cytotoxicity of different fractions of Alstonia scholaris R. Br. (ASE), the HeLa cells were treated with different doses of various fractions of ASE collected in monsoon, winter and summer. The exposure of HeLa cells to different extracts prepared from the stem bark collected in monsoon, winter and summer seasons resulted in a dose dependent increase in the cell killing effect of ASE and the highest cell killing effect was observed for the extract prepared from the summer collections. Similarly, treatment of HeLa cells with different doses of various fractions of the Alstonia scholaris extract viz. residue (ASERS), steroidal (ASEST), chloroform (ASECH), petroleum ether (ASEPE), diethyl ether (ASEDE), ethyl acetate (ASEEA), n-butanol (ASENB), aqueous (ASEAQ) and echitamine chloride (ECL) also resulted in a dose dependent decline in the cell viability, where the greatest cytotoxic effect was observed for residue (ASERS), followed by the whole extract (ASE) and chloroform (ASECH) fraction, while the least activity was observed for the steroidal (ASEST) fraction. The cytotoxicity declined ASERS > ASE > ASECH >ECL > ASEEA > ASEDE > ASEPE > ASENB > ASEAQ > ASEST in order. Our study demonstrates that the extract prepared from the summer collection, and the fractions containing the alkaloids were highly effective in cell killing. The extract of ASE was more powerful than the active principle echitamine present in ASE.

UR - http://www.scopus.com/inward/record.url?scp=9644304559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9644304559&partnerID=8YFLogxK

U2 - 10.1016/j.jep.2004.07.024

DO - 10.1016/j.jep.2004.07.024

M3 - Article

VL - 96

SP - 37

EP - 42

JO - Journal of Ethnopharmacology

JF - Journal of Ethnopharmacology

SN - 0378-8741

IS - 1-2

ER -