The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats

Manjeshwar Shrinath Baliga, Ganesh Chandra Jagetia, Jagadish N. Ulloor, Manjeshwar Poonam Baliga, Ponemone Venkatesh, Rosi Reddy, K. V N Mallikarjun Rao, Bantwal Shivanada Baliga, Sulochana Devi, Sudheer Kumar Raju, Veerapura Veeresh, Tiyyagura Koti Reddy, K. Laxminarayana Bairy

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The acute and sub-acute toxic effects of various doses of hydroalcoholic extract of Alstonia scholaris (ASE) was studied in mice and rats. The acute toxicity in mice depended on the season of collection of plant. The highest acute toxicity was observed in the ASE prepared from the summer collection followed by winter. The least toxicity was observed in the extract prepared from the bark of A. scholaris collected in the monsoon season. The administration of different doses of ASE showed a dose dependent increase in the toxicity in all species of mice. The Swiss albino mice were found to be the most sensitive followed by the DBA and C57BL. The crossbred mice were resistant when compared to the pure inbred strains. The oral administration of ASE was non-toxic up to a dose of 2000 mg/kg b. wt., while maximum number of animals succumbed to death after administration of 1100 mg/kg ASE by intraperitoneal route. The rats were more sensitive than the mice as the LD50 dose of ASE was lesser for the former than the latter. The sub-acute toxicity in the rats was carried out with 120 and 240 mg/kg b. wt. ASE (1/10th and 1/5th of the LD50 dose of ASE). The 240 mg was observed to be more toxic than 120 mg/kg ASE since it caused mortality and deformity in various organs of the recipient animals. The various biochemical parameters like AST, ALT, ACP, ALP, CK, LDH, creatinine, urea, ammonia, glucose and LPx were higher at 240 mg/kg ASE when compared with the 120 mg and the non-drug treated animals. In contrast, the total protein, albumin, DNA, RNA, cholesterol, glucose, glutathione, total thiols declined in the 240 mg/kg ASE treated animals when compared with non-drug treated controls. The hematological analysis showed a dose dependent decrease in the RBC, WBC, hemoglobin, neutrophils and monocytes, while a significant increase in the lymphocytes, eosinophils and basophils was observed. The observed toxic effect of ASE may be due to the presence of echitamine. Our studies shows that at high doses, A. scholaris exhibited marked damage to all the major organs of the body.

Original languageEnglish
Pages (from-to)317-326
Number of pages10
JournalToxicology Letters
Volume151
Issue number2
DOIs
Publication statusPublished - 15-07-2004
Externally publishedYes

Fingerprint

Alstonia
Toxicity
Rats
Poisons
Safety
Animals
Lethal Dose 50
Glucose
Lymphocytes
Animal Structures
Ammonia
Sulfhydryl Compounds
Basophils
Glutathione
Urea
Albumins
Creatinine
Eosinophils
Hemoglobins
Cholesterol

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Baliga, M. S., Jagetia, G. C., Ulloor, J. N., Baliga, M. P., Venkatesh, P., Reddy, R., ... Bairy, K. L. (2004). The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats. Toxicology Letters, 151(2), 317-326. https://doi.org/10.1016/j.toxlet.2004.01.015
Baliga, Manjeshwar Shrinath ; Jagetia, Ganesh Chandra ; Ulloor, Jagadish N. ; Baliga, Manjeshwar Poonam ; Venkatesh, Ponemone ; Reddy, Rosi ; Rao, K. V N Mallikarjun ; Baliga, Bantwal Shivanada ; Devi, Sulochana ; Raju, Sudheer Kumar ; Veeresh, Veerapura ; Reddy, Tiyyagura Koti ; Bairy, K. Laxminarayana. / The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats. In: Toxicology Letters. 2004 ; Vol. 151, No. 2. pp. 317-326.
@article{346995d424ca4bda9dabcfdcc13176a3,
title = "The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats",
abstract = "The acute and sub-acute toxic effects of various doses of hydroalcoholic extract of Alstonia scholaris (ASE) was studied in mice and rats. The acute toxicity in mice depended on the season of collection of plant. The highest acute toxicity was observed in the ASE prepared from the summer collection followed by winter. The least toxicity was observed in the extract prepared from the bark of A. scholaris collected in the monsoon season. The administration of different doses of ASE showed a dose dependent increase in the toxicity in all species of mice. The Swiss albino mice were found to be the most sensitive followed by the DBA and C57BL. The crossbred mice were resistant when compared to the pure inbred strains. The oral administration of ASE was non-toxic up to a dose of 2000 mg/kg b. wt., while maximum number of animals succumbed to death after administration of 1100 mg/kg ASE by intraperitoneal route. The rats were more sensitive than the mice as the LD50 dose of ASE was lesser for the former than the latter. The sub-acute toxicity in the rats was carried out with 120 and 240 mg/kg b. wt. ASE (1/10th and 1/5th of the LD50 dose of ASE). The 240 mg was observed to be more toxic than 120 mg/kg ASE since it caused mortality and deformity in various organs of the recipient animals. The various biochemical parameters like AST, ALT, ACP, ALP, CK, LDH, creatinine, urea, ammonia, glucose and LPx were higher at 240 mg/kg ASE when compared with the 120 mg and the non-drug treated animals. In contrast, the total protein, albumin, DNA, RNA, cholesterol, glucose, glutathione, total thiols declined in the 240 mg/kg ASE treated animals when compared with non-drug treated controls. The hematological analysis showed a dose dependent decrease in the RBC, WBC, hemoglobin, neutrophils and monocytes, while a significant increase in the lymphocytes, eosinophils and basophils was observed. The observed toxic effect of ASE may be due to the presence of echitamine. Our studies shows that at high doses, A. scholaris exhibited marked damage to all the major organs of the body.",
author = "Baliga, {Manjeshwar Shrinath} and Jagetia, {Ganesh Chandra} and Ulloor, {Jagadish N.} and Baliga, {Manjeshwar Poonam} and Ponemone Venkatesh and Rosi Reddy and Rao, {K. V N Mallikarjun} and Baliga, {Bantwal Shivanada} and Sulochana Devi and Raju, {Sudheer Kumar} and Veerapura Veeresh and Reddy, {Tiyyagura Koti} and Bairy, {K. Laxminarayana}",
year = "2004",
month = "7",
day = "15",
doi = "10.1016/j.toxlet.2004.01.015",
language = "English",
volume = "151",
pages = "317--326",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier BV",
number = "2",

}

Baliga, MS, Jagetia, GC, Ulloor, JN, Baliga, MP, Venkatesh, P, Reddy, R, Rao, KVNM, Baliga, BS, Devi, S, Raju, SK, Veeresh, V, Reddy, TK & Bairy, KL 2004, 'The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats', Toxicology Letters, vol. 151, no. 2, pp. 317-326. https://doi.org/10.1016/j.toxlet.2004.01.015

The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats. / Baliga, Manjeshwar Shrinath; Jagetia, Ganesh Chandra; Ulloor, Jagadish N.; Baliga, Manjeshwar Poonam; Venkatesh, Ponemone; Reddy, Rosi; Rao, K. V N Mallikarjun; Baliga, Bantwal Shivanada; Devi, Sulochana; Raju, Sudheer Kumar; Veeresh, Veerapura; Reddy, Tiyyagura Koti; Bairy, K. Laxminarayana.

In: Toxicology Letters, Vol. 151, No. 2, 15.07.2004, p. 317-326.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The evaluation of the acute toxicity and long term safety of hydroalcoholic extract of Sapthaparna (Alstonia scholaris) in mice and rats

AU - Baliga, Manjeshwar Shrinath

AU - Jagetia, Ganesh Chandra

AU - Ulloor, Jagadish N.

AU - Baliga, Manjeshwar Poonam

AU - Venkatesh, Ponemone

AU - Reddy, Rosi

AU - Rao, K. V N Mallikarjun

AU - Baliga, Bantwal Shivanada

AU - Devi, Sulochana

AU - Raju, Sudheer Kumar

AU - Veeresh, Veerapura

AU - Reddy, Tiyyagura Koti

AU - Bairy, K. Laxminarayana

PY - 2004/7/15

Y1 - 2004/7/15

N2 - The acute and sub-acute toxic effects of various doses of hydroalcoholic extract of Alstonia scholaris (ASE) was studied in mice and rats. The acute toxicity in mice depended on the season of collection of plant. The highest acute toxicity was observed in the ASE prepared from the summer collection followed by winter. The least toxicity was observed in the extract prepared from the bark of A. scholaris collected in the monsoon season. The administration of different doses of ASE showed a dose dependent increase in the toxicity in all species of mice. The Swiss albino mice were found to be the most sensitive followed by the DBA and C57BL. The crossbred mice were resistant when compared to the pure inbred strains. The oral administration of ASE was non-toxic up to a dose of 2000 mg/kg b. wt., while maximum number of animals succumbed to death after administration of 1100 mg/kg ASE by intraperitoneal route. The rats were more sensitive than the mice as the LD50 dose of ASE was lesser for the former than the latter. The sub-acute toxicity in the rats was carried out with 120 and 240 mg/kg b. wt. ASE (1/10th and 1/5th of the LD50 dose of ASE). The 240 mg was observed to be more toxic than 120 mg/kg ASE since it caused mortality and deformity in various organs of the recipient animals. The various biochemical parameters like AST, ALT, ACP, ALP, CK, LDH, creatinine, urea, ammonia, glucose and LPx were higher at 240 mg/kg ASE when compared with the 120 mg and the non-drug treated animals. In contrast, the total protein, albumin, DNA, RNA, cholesterol, glucose, glutathione, total thiols declined in the 240 mg/kg ASE treated animals when compared with non-drug treated controls. The hematological analysis showed a dose dependent decrease in the RBC, WBC, hemoglobin, neutrophils and monocytes, while a significant increase in the lymphocytes, eosinophils and basophils was observed. The observed toxic effect of ASE may be due to the presence of echitamine. Our studies shows that at high doses, A. scholaris exhibited marked damage to all the major organs of the body.

AB - The acute and sub-acute toxic effects of various doses of hydroalcoholic extract of Alstonia scholaris (ASE) was studied in mice and rats. The acute toxicity in mice depended on the season of collection of plant. The highest acute toxicity was observed in the ASE prepared from the summer collection followed by winter. The least toxicity was observed in the extract prepared from the bark of A. scholaris collected in the monsoon season. The administration of different doses of ASE showed a dose dependent increase in the toxicity in all species of mice. The Swiss albino mice were found to be the most sensitive followed by the DBA and C57BL. The crossbred mice were resistant when compared to the pure inbred strains. The oral administration of ASE was non-toxic up to a dose of 2000 mg/kg b. wt., while maximum number of animals succumbed to death after administration of 1100 mg/kg ASE by intraperitoneal route. The rats were more sensitive than the mice as the LD50 dose of ASE was lesser for the former than the latter. The sub-acute toxicity in the rats was carried out with 120 and 240 mg/kg b. wt. ASE (1/10th and 1/5th of the LD50 dose of ASE). The 240 mg was observed to be more toxic than 120 mg/kg ASE since it caused mortality and deformity in various organs of the recipient animals. The various biochemical parameters like AST, ALT, ACP, ALP, CK, LDH, creatinine, urea, ammonia, glucose and LPx were higher at 240 mg/kg ASE when compared with the 120 mg and the non-drug treated animals. In contrast, the total protein, albumin, DNA, RNA, cholesterol, glucose, glutathione, total thiols declined in the 240 mg/kg ASE treated animals when compared with non-drug treated controls. The hematological analysis showed a dose dependent decrease in the RBC, WBC, hemoglobin, neutrophils and monocytes, while a significant increase in the lymphocytes, eosinophils and basophils was observed. The observed toxic effect of ASE may be due to the presence of echitamine. Our studies shows that at high doses, A. scholaris exhibited marked damage to all the major organs of the body.

UR - http://www.scopus.com/inward/record.url?scp=2942556915&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942556915&partnerID=8YFLogxK

U2 - 10.1016/j.toxlet.2004.01.015

DO - 10.1016/j.toxlet.2004.01.015

M3 - Article

VL - 151

SP - 317

EP - 326

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - 2

ER -