The grapefruit flavanone naringin protects against the radiation-induced genomic instability in the mice bone marrow

A micronucleus study

Ganesh Chandra Jagetia, Tiyyagura Koti Reddy

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The effect of various doses, viz. 0, 0.5, 1, 2, 4, 6 and 8mg/kg body weight of naringin (NIN) (a citrus flavanone) was studied on the alteration in the radiation-induced micronucleated polychromatic (MPCE) and normochromatic (MNCE) erythrocytes in mouse bone marrow exposed to 2Gy of 60Co γ-radiation. The treatment of mice with various doses of NIN before exposure to 2Gy resulted in a significant decline in the frequency of MPCE when compared to the non-drug-treated irradiated control. However, the greatest reduction in MPCE was observed for 2mg/kg body weight NIN, accompanied by a highest PCE/NCE ratio when compared with the non-drug-treated irradiated control. Therefore, further studies were carried out using this dose of NIN, where the animals were administered with 2mg/kg body weight of NIN before exposure to 0, 0.5, 1, 2, 3 and 4Gy of γ-radiation. The frequency of MPCE and MNCE increased in a dose-dependent manner in both the non-drug-treated irradiated control and NIN-pretreated irradiated groups up to a dose of 2Gy, while a further increase in the irradiation dose resulted in a significant decline in MPCE and MNCE frequencies in both groups. Pretreatment of mice with 2mg/kg body weight of NIN resulted in a significant decline in the frequencies of MPCE and MNCE. NIN treatment not only reduced the frequency of MPCE with one micronucleus, but also of MPCE with multiple micronuclei (MN), indicating its ability to reduce complex chromosome aberrations. Conversely, the PCE/NCE ratio declined in a dose-dependent manner in both groups. The treatment of mice with NIN before exposure to different doses of γ-radiation resulted in the inhibition in this decline in the PCE/NCE ratio. Our study demonstrates that NIN is able to protect mouse bone marrow cells against the radiation-induced DNA damage and decline in the cell proliferation as observed by a reduction in the micronucleus frequency and an increase in PCE/NCE ratio, respectively, in the NIN-pretreated irradiated group.

Original languageEnglish
Pages (from-to)37-48
Number of pages12
JournalMutation Research - Genetic Toxicology and Environmental Mutagenesis
Volume519
Issue number1-2
DOIs
Publication statusPublished - 26-08-2002
Externally publishedYes

Fingerprint

Citrus paradisi
Genomic Instability
Bone Marrow
Radiation
Body Weight
flavanone
naringin
Citrus
Chromosome Aberrations
Bone Marrow Cells
DNA Damage
Erythrocytes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Health, Toxicology and Mutagenesis

Cite this

@article{e67c9ef8cd4249e1a737afb5f05430b0,
title = "The grapefruit flavanone naringin protects against the radiation-induced genomic instability in the mice bone marrow: A micronucleus study",
abstract = "The effect of various doses, viz. 0, 0.5, 1, 2, 4, 6 and 8mg/kg body weight of naringin (NIN) (a citrus flavanone) was studied on the alteration in the radiation-induced micronucleated polychromatic (MPCE) and normochromatic (MNCE) erythrocytes in mouse bone marrow exposed to 2Gy of 60Co γ-radiation. The treatment of mice with various doses of NIN before exposure to 2Gy resulted in a significant decline in the frequency of MPCE when compared to the non-drug-treated irradiated control. However, the greatest reduction in MPCE was observed for 2mg/kg body weight NIN, accompanied by a highest PCE/NCE ratio when compared with the non-drug-treated irradiated control. Therefore, further studies were carried out using this dose of NIN, where the animals were administered with 2mg/kg body weight of NIN before exposure to 0, 0.5, 1, 2, 3 and 4Gy of γ-radiation. The frequency of MPCE and MNCE increased in a dose-dependent manner in both the non-drug-treated irradiated control and NIN-pretreated irradiated groups up to a dose of 2Gy, while a further increase in the irradiation dose resulted in a significant decline in MPCE and MNCE frequencies in both groups. Pretreatment of mice with 2mg/kg body weight of NIN resulted in a significant decline in the frequencies of MPCE and MNCE. NIN treatment not only reduced the frequency of MPCE with one micronucleus, but also of MPCE with multiple micronuclei (MN), indicating its ability to reduce complex chromosome aberrations. Conversely, the PCE/NCE ratio declined in a dose-dependent manner in both groups. The treatment of mice with NIN before exposure to different doses of γ-radiation resulted in the inhibition in this decline in the PCE/NCE ratio. Our study demonstrates that NIN is able to protect mouse bone marrow cells against the radiation-induced DNA damage and decline in the cell proliferation as observed by a reduction in the micronucleus frequency and an increase in PCE/NCE ratio, respectively, in the NIN-pretreated irradiated group.",
author = "Jagetia, {Ganesh Chandra} and Reddy, {Tiyyagura Koti}",
year = "2002",
month = "8",
day = "26",
doi = "10.1016/S1383-5718(02)00111-0",
language = "English",
volume = "519",
pages = "37--48",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",
publisher = "Elsevier",
number = "1-2",

}

The grapefruit flavanone naringin protects against the radiation-induced genomic instability in the mice bone marrow : A micronucleus study. / Jagetia, Ganesh Chandra; Reddy, Tiyyagura Koti.

In: Mutation Research - Genetic Toxicology and Environmental Mutagenesis, Vol. 519, No. 1-2, 26.08.2002, p. 37-48.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The grapefruit flavanone naringin protects against the radiation-induced genomic instability in the mice bone marrow

T2 - A micronucleus study

AU - Jagetia, Ganesh Chandra

AU - Reddy, Tiyyagura Koti

PY - 2002/8/26

Y1 - 2002/8/26

N2 - The effect of various doses, viz. 0, 0.5, 1, 2, 4, 6 and 8mg/kg body weight of naringin (NIN) (a citrus flavanone) was studied on the alteration in the radiation-induced micronucleated polychromatic (MPCE) and normochromatic (MNCE) erythrocytes in mouse bone marrow exposed to 2Gy of 60Co γ-radiation. The treatment of mice with various doses of NIN before exposure to 2Gy resulted in a significant decline in the frequency of MPCE when compared to the non-drug-treated irradiated control. However, the greatest reduction in MPCE was observed for 2mg/kg body weight NIN, accompanied by a highest PCE/NCE ratio when compared with the non-drug-treated irradiated control. Therefore, further studies were carried out using this dose of NIN, where the animals were administered with 2mg/kg body weight of NIN before exposure to 0, 0.5, 1, 2, 3 and 4Gy of γ-radiation. The frequency of MPCE and MNCE increased in a dose-dependent manner in both the non-drug-treated irradiated control and NIN-pretreated irradiated groups up to a dose of 2Gy, while a further increase in the irradiation dose resulted in a significant decline in MPCE and MNCE frequencies in both groups. Pretreatment of mice with 2mg/kg body weight of NIN resulted in a significant decline in the frequencies of MPCE and MNCE. NIN treatment not only reduced the frequency of MPCE with one micronucleus, but also of MPCE with multiple micronuclei (MN), indicating its ability to reduce complex chromosome aberrations. Conversely, the PCE/NCE ratio declined in a dose-dependent manner in both groups. The treatment of mice with NIN before exposure to different doses of γ-radiation resulted in the inhibition in this decline in the PCE/NCE ratio. Our study demonstrates that NIN is able to protect mouse bone marrow cells against the radiation-induced DNA damage and decline in the cell proliferation as observed by a reduction in the micronucleus frequency and an increase in PCE/NCE ratio, respectively, in the NIN-pretreated irradiated group.

AB - The effect of various doses, viz. 0, 0.5, 1, 2, 4, 6 and 8mg/kg body weight of naringin (NIN) (a citrus flavanone) was studied on the alteration in the radiation-induced micronucleated polychromatic (MPCE) and normochromatic (MNCE) erythrocytes in mouse bone marrow exposed to 2Gy of 60Co γ-radiation. The treatment of mice with various doses of NIN before exposure to 2Gy resulted in a significant decline in the frequency of MPCE when compared to the non-drug-treated irradiated control. However, the greatest reduction in MPCE was observed for 2mg/kg body weight NIN, accompanied by a highest PCE/NCE ratio when compared with the non-drug-treated irradiated control. Therefore, further studies were carried out using this dose of NIN, where the animals were administered with 2mg/kg body weight of NIN before exposure to 0, 0.5, 1, 2, 3 and 4Gy of γ-radiation. The frequency of MPCE and MNCE increased in a dose-dependent manner in both the non-drug-treated irradiated control and NIN-pretreated irradiated groups up to a dose of 2Gy, while a further increase in the irradiation dose resulted in a significant decline in MPCE and MNCE frequencies in both groups. Pretreatment of mice with 2mg/kg body weight of NIN resulted in a significant decline in the frequencies of MPCE and MNCE. NIN treatment not only reduced the frequency of MPCE with one micronucleus, but also of MPCE with multiple micronuclei (MN), indicating its ability to reduce complex chromosome aberrations. Conversely, the PCE/NCE ratio declined in a dose-dependent manner in both groups. The treatment of mice with NIN before exposure to different doses of γ-radiation resulted in the inhibition in this decline in the PCE/NCE ratio. Our study demonstrates that NIN is able to protect mouse bone marrow cells against the radiation-induced DNA damage and decline in the cell proliferation as observed by a reduction in the micronucleus frequency and an increase in PCE/NCE ratio, respectively, in the NIN-pretreated irradiated group.

UR - http://www.scopus.com/inward/record.url?scp=0037179205&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037179205&partnerID=8YFLogxK

U2 - 10.1016/S1383-5718(02)00111-0

DO - 10.1016/S1383-5718(02)00111-0

M3 - Article

VL - 519

SP - 37

EP - 48

JO - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research - Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

IS - 1-2

ER -