The Influence of Vinblastine Treatment on the Formation of Radiation‐Induced Micronuclei in Mouse Bone Marrow

Ganesh Chandra Jagetia, Preenu Sunil Jacob

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The combined effects of vinblastine sulphate (VBL) and gamma radiation treatments on the induction of micronuclei in polychromatic erythrocytes (MPCE) and normochromatic erythrocytes (MNCE) and changes in polychromatic/normochromatic erythrocyte ratio (P/N ratio) in mouse bone marrow, were studied. Compared to double distilled water (DDW) injected controls, 0.05 mg/kg b.wt. of vinblastine alone significantly increased the frequency of MPCE and MNCE. When VBL was administered before exposure to various doses of gamma radiation the frequency of micronuclei (MPCE and MNCE) increased with the increasing radiation dose up to a dose of 4 Gy, but this increase levelled off at 3 to 4 Gy irradiation. The frequency of MPCE was found to be significantly lower at 3 and 4 Gy radiation when compared to the DDW + irradiated group. A significant dose dependent decline in the P/N ratio was observed in the DDW + irradiated group. VBL treatment before irradiation resulted in a more significant decline in the P/N ratio than that of DDW + irradiated group. The formation of micronuclei (MPCE and MNCE) increased from 8 h post‐exposure and continued to increase up to 28 h. There was a sharp decline in the frequency of micronuclei (MPCE and MNCE) at 32 h post‐irradiation, in both DDW + 1 Gy irradiated and VBL + 1 Gy irradiated groups; then it declined gradually up to 72 h post‐exposure without restoration to normal level. The P/N ratio declined with time in all three groups (VBL alone, DDW + irradiation, and VBL + irradiation) without restoration to normal level up to 72 h post‐treatment, except in the DDW 4‐ irradiated group, where it was normal.

Original languageEnglish
Pages (from-to)51-59
Number of pages9
JournalHereditas
Volume120
Issue number1
DOIs
Publication statusPublished - 01-01-1994
Externally publishedYes

Fingerprint

Vinblastine
Erythrocytes
Bone Marrow
Water
Gamma Rays
Radiation Dosage

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

Jagetia, Ganesh Chandra ; Jacob, Preenu Sunil. / The Influence of Vinblastine Treatment on the Formation of Radiation‐Induced Micronuclei in Mouse Bone Marrow. In: Hereditas. 1994 ; Vol. 120, No. 1. pp. 51-59.
@article{7520f617677247bf8cfe8f3b27df0c5e,
title = "The Influence of Vinblastine Treatment on the Formation of Radiation‐Induced Micronuclei in Mouse Bone Marrow",
abstract = "The combined effects of vinblastine sulphate (VBL) and gamma radiation treatments on the induction of micronuclei in polychromatic erythrocytes (MPCE) and normochromatic erythrocytes (MNCE) and changes in polychromatic/normochromatic erythrocyte ratio (P/N ratio) in mouse bone marrow, were studied. Compared to double distilled water (DDW) injected controls, 0.05 mg/kg b.wt. of vinblastine alone significantly increased the frequency of MPCE and MNCE. When VBL was administered before exposure to various doses of gamma radiation the frequency of micronuclei (MPCE and MNCE) increased with the increasing radiation dose up to a dose of 4 Gy, but this increase levelled off at 3 to 4 Gy irradiation. The frequency of MPCE was found to be significantly lower at 3 and 4 Gy radiation when compared to the DDW + irradiated group. A significant dose dependent decline in the P/N ratio was observed in the DDW + irradiated group. VBL treatment before irradiation resulted in a more significant decline in the P/N ratio than that of DDW + irradiated group. The formation of micronuclei (MPCE and MNCE) increased from 8 h post‐exposure and continued to increase up to 28 h. There was a sharp decline in the frequency of micronuclei (MPCE and MNCE) at 32 h post‐irradiation, in both DDW + 1 Gy irradiated and VBL + 1 Gy irradiated groups; then it declined gradually up to 72 h post‐exposure without restoration to normal level. The P/N ratio declined with time in all three groups (VBL alone, DDW + irradiation, and VBL + irradiation) without restoration to normal level up to 72 h post‐treatment, except in the DDW 4‐ irradiated group, where it was normal.",
author = "Jagetia, {Ganesh Chandra} and Jacob, {Preenu Sunil}",
year = "1994",
month = "1",
day = "1",
doi = "10.1111/j.1601-5223.1994.00051.x",
language = "English",
volume = "120",
pages = "51--59",
journal = "Hereditas",
issn = "0018-0661",
publisher = "Wiley-Blackwell",
number = "1",

}

The Influence of Vinblastine Treatment on the Formation of Radiation‐Induced Micronuclei in Mouse Bone Marrow. / Jagetia, Ganesh Chandra; Jacob, Preenu Sunil.

In: Hereditas, Vol. 120, No. 1, 01.01.1994, p. 51-59.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The Influence of Vinblastine Treatment on the Formation of Radiation‐Induced Micronuclei in Mouse Bone Marrow

AU - Jagetia, Ganesh Chandra

AU - Jacob, Preenu Sunil

PY - 1994/1/1

Y1 - 1994/1/1

N2 - The combined effects of vinblastine sulphate (VBL) and gamma radiation treatments on the induction of micronuclei in polychromatic erythrocytes (MPCE) and normochromatic erythrocytes (MNCE) and changes in polychromatic/normochromatic erythrocyte ratio (P/N ratio) in mouse bone marrow, were studied. Compared to double distilled water (DDW) injected controls, 0.05 mg/kg b.wt. of vinblastine alone significantly increased the frequency of MPCE and MNCE. When VBL was administered before exposure to various doses of gamma radiation the frequency of micronuclei (MPCE and MNCE) increased with the increasing radiation dose up to a dose of 4 Gy, but this increase levelled off at 3 to 4 Gy irradiation. The frequency of MPCE was found to be significantly lower at 3 and 4 Gy radiation when compared to the DDW + irradiated group. A significant dose dependent decline in the P/N ratio was observed in the DDW + irradiated group. VBL treatment before irradiation resulted in a more significant decline in the P/N ratio than that of DDW + irradiated group. The formation of micronuclei (MPCE and MNCE) increased from 8 h post‐exposure and continued to increase up to 28 h. There was a sharp decline in the frequency of micronuclei (MPCE and MNCE) at 32 h post‐irradiation, in both DDW + 1 Gy irradiated and VBL + 1 Gy irradiated groups; then it declined gradually up to 72 h post‐exposure without restoration to normal level. The P/N ratio declined with time in all three groups (VBL alone, DDW + irradiation, and VBL + irradiation) without restoration to normal level up to 72 h post‐treatment, except in the DDW 4‐ irradiated group, where it was normal.

AB - The combined effects of vinblastine sulphate (VBL) and gamma radiation treatments on the induction of micronuclei in polychromatic erythrocytes (MPCE) and normochromatic erythrocytes (MNCE) and changes in polychromatic/normochromatic erythrocyte ratio (P/N ratio) in mouse bone marrow, were studied. Compared to double distilled water (DDW) injected controls, 0.05 mg/kg b.wt. of vinblastine alone significantly increased the frequency of MPCE and MNCE. When VBL was administered before exposure to various doses of gamma radiation the frequency of micronuclei (MPCE and MNCE) increased with the increasing radiation dose up to a dose of 4 Gy, but this increase levelled off at 3 to 4 Gy irradiation. The frequency of MPCE was found to be significantly lower at 3 and 4 Gy radiation when compared to the DDW + irradiated group. A significant dose dependent decline in the P/N ratio was observed in the DDW + irradiated group. VBL treatment before irradiation resulted in a more significant decline in the P/N ratio than that of DDW + irradiated group. The formation of micronuclei (MPCE and MNCE) increased from 8 h post‐exposure and continued to increase up to 28 h. There was a sharp decline in the frequency of micronuclei (MPCE and MNCE) at 32 h post‐irradiation, in both DDW + 1 Gy irradiated and VBL + 1 Gy irradiated groups; then it declined gradually up to 72 h post‐exposure without restoration to normal level. The P/N ratio declined with time in all three groups (VBL alone, DDW + irradiation, and VBL + irradiation) without restoration to normal level up to 72 h post‐treatment, except in the DDW 4‐ irradiated group, where it was normal.

UR - http://www.scopus.com/inward/record.url?scp=0028348384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028348384&partnerID=8YFLogxK

U2 - 10.1111/j.1601-5223.1994.00051.x

DO - 10.1111/j.1601-5223.1994.00051.x

M3 - Article

C2 - 8206784

AN - SCOPUS:0028348384

VL - 120

SP - 51

EP - 59

JO - Hereditas

JF - Hereditas

SN - 0018-0661

IS - 1

ER -