TY - JOUR
T1 - The regulatory role of neurotransmitters in orexin B induced nucleus accumbens dependent alcohol ingestion in wistar albino rats
AU - Rashmi, K. S.
AU - Maynnavar, Santhosh K.
AU - Arunkumar, Nayanatara
AU - Bhagyalakshmi, K.
AU - Poojary, Dharnappa
AU - Kadur, Kishan
AU - Vinodini, N. A.
AU - Bolumbu, Ganaraja
N1 - Funding Information:
Department of Biotechnology, Ministry of Science and Technology, New Delhi, Government of India.
Publisher Copyright:
© RJPT All right reserved.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2020/12
Y1 - 2020/12
N2 - Objective: The Present study mainly focussed on the influential role of local neurotransmitter level in Orexin B induced Nucleus Accumbens (NAc) dependent voluntary ethanol consumption and preference. Methods: NAc in male Wistar rats were infused by Orexin B (low dose-3 nmol/μl and high dose 30nmol/μl) and Orexin B antagonist (TCS-OX2-29) (10μg/μl) sequentially. Food intake, alcohol intake, and two-bottle free choice preference tests (alcohol vs. water) were carried out at one hour, 2 hrs, 4 hrs, 12 hours, and 24 hrs in all the groups. Neurotransmitters level in the Nucleus Accumbens was also estimated at the end of the study. Results: Orexin B (30nm/µl) increased net alcohol intake in single-bottle test as well as in two-bottle free choice test. Whereas, Orexin B antagonist decreased alcohol consumption and preference to ethanol. Glutamate level significantly decreased and Dopamine and noradrenaline concomitantly increased. Conclusions: Orexin B plays a role in the modulation of ethanol consumption and preference confirmed by Orexin B antagonist infusion. The influence of neurotransmitter levels in Orexin B mediated ethanol consumption was also explored.
AB - Objective: The Present study mainly focussed on the influential role of local neurotransmitter level in Orexin B induced Nucleus Accumbens (NAc) dependent voluntary ethanol consumption and preference. Methods: NAc in male Wistar rats were infused by Orexin B (low dose-3 nmol/μl and high dose 30nmol/μl) and Orexin B antagonist (TCS-OX2-29) (10μg/μl) sequentially. Food intake, alcohol intake, and two-bottle free choice preference tests (alcohol vs. water) were carried out at one hour, 2 hrs, 4 hrs, 12 hours, and 24 hrs in all the groups. Neurotransmitters level in the Nucleus Accumbens was also estimated at the end of the study. Results: Orexin B (30nm/µl) increased net alcohol intake in single-bottle test as well as in two-bottle free choice test. Whereas, Orexin B antagonist decreased alcohol consumption and preference to ethanol. Glutamate level significantly decreased and Dopamine and noradrenaline concomitantly increased. Conclusions: Orexin B plays a role in the modulation of ethanol consumption and preference confirmed by Orexin B antagonist infusion. The influence of neurotransmitter levels in Orexin B mediated ethanol consumption was also explored.
UR - http://www.scopus.com/inward/record.url?scp=85100444379&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85100444379&partnerID=8YFLogxK
U2 - 10.5958/0974-360X.2020.01085.9
DO - 10.5958/0974-360X.2020.01085.9
M3 - Article
AN - SCOPUS:85100444379
SN - 0974-3618
VL - 13
SP - 6224
EP - 6230
JO - Research Journal of Pharmacy and Technology
JF - Research Journal of Pharmacy and Technology
IS - 12
ER -