The role of alanine 163 in solute permeability of Leishmania major aquaglyceroporin LmAQP1

Rita Mukhopadhyay, Goutam Mandal, Venkata Subba Rao Atluri, Katherine Figarella, Nestor L. Uzcategui, Yao Zhou, Eric Beitz, A. Abdul Ajees, Hiranmoy Bhattacharjee

Research output: Contribution to journalArticle

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Abstract

Leishmania major aquaglyceroporin LmAQP1 allows adventitious passage of antimonite, an activated form of the drug Pentostam, which is used as the first line treatment for leishmaniasis. The extracellular C-loop of an aquaglyceroporin confers substrate specificity. Alteration of Glu125 to serine in the Plasmodium falciparum aquaglyceroporin PfAQP has been shown to selectively affect water but not glycerol permeability. The C-loop of LmAQP1 is twelve residues longer than PfAQP, and Ala163 is at an equivalent position as Glu125 of PfAQP. The role of Ala163 in LmAQP1 solute permeability was investigated. Alteration of Ala163 to serine or threonine did not significantly affect conduction of solutes. However, alteration to aspartate, glutamate, and glutamine blocked passage of water, glycerol, and other organic solutes. While LmAQP1 is a mercurial insensitive water channel, mutation of the adjacent threonine (Thr164) to cysteine led to inhibition of water passage by Hg2+. This inhibition could be reversed upon addition of β-mercaptoethanol. These data suggest that, unlike Glu125 (PfAQP), Ala163 is not involved in stabilization of the C-loop and selective solute permeability. Ala163 is located near the pore mouth of the channel, and replacement of Ala163 by bulkier residue sterically hinders the passage of solutes. Alteration of Ala163 to serine or threonine affected metalloid uptake in the order, wild-type>A163S>A163T. Metalloid conduction was near completely blocked when Ala163 was mutagenized to aspartate, glutamate, or glutamine. Mutations such as A163S and A163T that reduced the permeability to antimonite, without a significant loss in water or solute conductivity raises the possibility that, subtle changes in the side chain of the amino acid residue in position 163 of LmAQP1 may play a role in drug resistance.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalMolecular and Biochemical Parasitology
Volume175
Issue number1
DOIs
Publication statusPublished - 01-01-2011

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Aquaglyceroporins
Leishmania major
Alanine
Permeability
Threonine
Metalloids
Serine
Water
Glutamine
Aspartic Acid
Glycerol
Glutamic Acid
Antimony Sodium Gluconate
Mutation
Leishmaniasis
Mercaptoethanol
Substrate Specificity
Drug Resistance
Cysteine
Mouth

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Parasitology

Cite this

Mukhopadhyay, R., Mandal, G., Atluri, V. S. R., Figarella, K., Uzcategui, N. L., Zhou, Y., ... Bhattacharjee, H. (2011). The role of alanine 163 in solute permeability of Leishmania major aquaglyceroporin LmAQP1. Molecular and Biochemical Parasitology, 175(1), 83-90. https://doi.org/10.1016/j.molbiopara.2010.09.007
Mukhopadhyay, Rita ; Mandal, Goutam ; Atluri, Venkata Subba Rao ; Figarella, Katherine ; Uzcategui, Nestor L. ; Zhou, Yao ; Beitz, Eric ; Ajees, A. Abdul ; Bhattacharjee, Hiranmoy. / The role of alanine 163 in solute permeability of Leishmania major aquaglyceroporin LmAQP1. In: Molecular and Biochemical Parasitology. 2011 ; Vol. 175, No. 1. pp. 83-90.
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abstract = "Leishmania major aquaglyceroporin LmAQP1 allows adventitious passage of antimonite, an activated form of the drug Pentostam, which is used as the first line treatment for leishmaniasis. The extracellular C-loop of an aquaglyceroporin confers substrate specificity. Alteration of Glu125 to serine in the Plasmodium falciparum aquaglyceroporin PfAQP has been shown to selectively affect water but not glycerol permeability. The C-loop of LmAQP1 is twelve residues longer than PfAQP, and Ala163 is at an equivalent position as Glu125 of PfAQP. The role of Ala163 in LmAQP1 solute permeability was investigated. Alteration of Ala163 to serine or threonine did not significantly affect conduction of solutes. However, alteration to aspartate, glutamate, and glutamine blocked passage of water, glycerol, and other organic solutes. While LmAQP1 is a mercurial insensitive water channel, mutation of the adjacent threonine (Thr164) to cysteine led to inhibition of water passage by Hg2+. This inhibition could be reversed upon addition of β-mercaptoethanol. These data suggest that, unlike Glu125 (PfAQP), Ala163 is not involved in stabilization of the C-loop and selective solute permeability. Ala163 is located near the pore mouth of the channel, and replacement of Ala163 by bulkier residue sterically hinders the passage of solutes. Alteration of Ala163 to serine or threonine affected metalloid uptake in the order, wild-type>A163S>A163T. Metalloid conduction was near completely blocked when Ala163 was mutagenized to aspartate, glutamate, or glutamine. Mutations such as A163S and A163T that reduced the permeability to antimonite, without a significant loss in water or solute conductivity raises the possibility that, subtle changes in the side chain of the amino acid residue in position 163 of LmAQP1 may play a role in drug resistance.",
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Mukhopadhyay, R, Mandal, G, Atluri, VSR, Figarella, K, Uzcategui, NL, Zhou, Y, Beitz, E, Ajees, AA & Bhattacharjee, H 2011, 'The role of alanine 163 in solute permeability of Leishmania major aquaglyceroporin LmAQP1', Molecular and Biochemical Parasitology, vol. 175, no. 1, pp. 83-90. https://doi.org/10.1016/j.molbiopara.2010.09.007

The role of alanine 163 in solute permeability of Leishmania major aquaglyceroporin LmAQP1. / Mukhopadhyay, Rita; Mandal, Goutam; Atluri, Venkata Subba Rao; Figarella, Katherine; Uzcategui, Nestor L.; Zhou, Yao; Beitz, Eric; Ajees, A. Abdul; Bhattacharjee, Hiranmoy.

In: Molecular and Biochemical Parasitology, Vol. 175, No. 1, 01.01.2011, p. 83-90.

Research output: Contribution to journalArticle

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T1 - The role of alanine 163 in solute permeability of Leishmania major aquaglyceroporin LmAQP1

AU - Mukhopadhyay, Rita

AU - Mandal, Goutam

AU - Atluri, Venkata Subba Rao

AU - Figarella, Katherine

AU - Uzcategui, Nestor L.

AU - Zhou, Yao

AU - Beitz, Eric

AU - Ajees, A. Abdul

AU - Bhattacharjee, Hiranmoy

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N2 - Leishmania major aquaglyceroporin LmAQP1 allows adventitious passage of antimonite, an activated form of the drug Pentostam, which is used as the first line treatment for leishmaniasis. The extracellular C-loop of an aquaglyceroporin confers substrate specificity. Alteration of Glu125 to serine in the Plasmodium falciparum aquaglyceroporin PfAQP has been shown to selectively affect water but not glycerol permeability. The C-loop of LmAQP1 is twelve residues longer than PfAQP, and Ala163 is at an equivalent position as Glu125 of PfAQP. The role of Ala163 in LmAQP1 solute permeability was investigated. Alteration of Ala163 to serine or threonine did not significantly affect conduction of solutes. However, alteration to aspartate, glutamate, and glutamine blocked passage of water, glycerol, and other organic solutes. While LmAQP1 is a mercurial insensitive water channel, mutation of the adjacent threonine (Thr164) to cysteine led to inhibition of water passage by Hg2+. This inhibition could be reversed upon addition of β-mercaptoethanol. These data suggest that, unlike Glu125 (PfAQP), Ala163 is not involved in stabilization of the C-loop and selective solute permeability. Ala163 is located near the pore mouth of the channel, and replacement of Ala163 by bulkier residue sterically hinders the passage of solutes. Alteration of Ala163 to serine or threonine affected metalloid uptake in the order, wild-type>A163S>A163T. Metalloid conduction was near completely blocked when Ala163 was mutagenized to aspartate, glutamate, or glutamine. Mutations such as A163S and A163T that reduced the permeability to antimonite, without a significant loss in water or solute conductivity raises the possibility that, subtle changes in the side chain of the amino acid residue in position 163 of LmAQP1 may play a role in drug resistance.

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