The Role of Natural Medicines on Wound Healing

A Biomechanical, Histological, Biochemical and Molecular Study

Sushma Kotian, Kumar Bhat, Sreedhara Pai, Jayakrishna Nayak, Anne Souza, Karthik Gourisheti, Divya Padma

Research output: Contribution to journalArticle

Abstract

Background: Traditional medicinal systems like Ayurveda and Indian folk medicine have used Honey, Ghee, Glycyrrhiza glabra, and Nerium indicum effectively for treating wounds. The known result of these medications is faster healing. However, the mechanism of actions at the tissue level, the biochemical and molecular mechanisms of healing is not well explored and documented. This present study was therefore designed to study the efficacy of these traditional medicines singly and in combinations on excision wounds in Wistar rats. Methods: At two different intervals (i.e., day 8 and day 16), biomechanical, histological and immunohistochemical (IHC) parameters were assessed at the wound site. IHC focused on the inflammatory rate by evaluating the level of cytokine, IL1β and the tissue remodeling by studying the activity of myofibroblasts. Results: Rapid epithelization, better remodeling, favorable inflammatory changes and an adequate myofibroblast activity at the wound site was observed in all the treated groups compared to control. Conclusion: This study is therefore useful in exploring the mechanism of action of these traditional medicines and providing valuable scientific evidence.

Original languageEnglish
Pages (from-to)759-770
Number of pages12
JournalEthiopian journal of health sciences
Volume28
Issue number6
DOIs
Publication statusPublished - 01-11-2018

Fingerprint

Wound Healing
Traditional Medicine
Myofibroblasts
Wounds and Injuries
Nerium
Glycyrrhiza
Honey
Wistar Rats
Cytokines

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Kotian, Sushma ; Bhat, Kumar ; Pai, Sreedhara ; Nayak, Jayakrishna ; Souza, Anne ; Gourisheti, Karthik ; Padma, Divya. / The Role of Natural Medicines on Wound Healing : A Biomechanical, Histological, Biochemical and Molecular Study. In: Ethiopian journal of health sciences. 2018 ; Vol. 28, No. 6. pp. 759-770.
@article{8365b41458454c55916a6f70495acb83,
title = "The Role of Natural Medicines on Wound Healing: A Biomechanical, Histological, Biochemical and Molecular Study",
abstract = "Background: Traditional medicinal systems like Ayurveda and Indian folk medicine have used Honey, Ghee, Glycyrrhiza glabra, and Nerium indicum effectively for treating wounds. The known result of these medications is faster healing. However, the mechanism of actions at the tissue level, the biochemical and molecular mechanisms of healing is not well explored and documented. This present study was therefore designed to study the efficacy of these traditional medicines singly and in combinations on excision wounds in Wistar rats. Methods: At two different intervals (i.e., day 8 and day 16), biomechanical, histological and immunohistochemical (IHC) parameters were assessed at the wound site. IHC focused on the inflammatory rate by evaluating the level of cytokine, IL1β and the tissue remodeling by studying the activity of myofibroblasts. Results: Rapid epithelization, better remodeling, favorable inflammatory changes and an adequate myofibroblast activity at the wound site was observed in all the treated groups compared to control. Conclusion: This study is therefore useful in exploring the mechanism of action of these traditional medicines and providing valuable scientific evidence.",
author = "Sushma Kotian and Kumar Bhat and Sreedhara Pai and Jayakrishna Nayak and Anne Souza and Karthik Gourisheti and Divya Padma",
year = "2018",
month = "11",
day = "1",
doi = "10.4314/ejhs.v28i6.11",
language = "English",
volume = "28",
pages = "759--770",
journal = "Ethiopian journal of health sciences",
issn = "1029-1857",
publisher = "Research and publications office, Jimma University",
number = "6",

}

The Role of Natural Medicines on Wound Healing : A Biomechanical, Histological, Biochemical and Molecular Study. / Kotian, Sushma; Bhat, Kumar; Pai, Sreedhara; Nayak, Jayakrishna; Souza, Anne; Gourisheti, Karthik; Padma, Divya.

In: Ethiopian journal of health sciences, Vol. 28, No. 6, 01.11.2018, p. 759-770.

Research output: Contribution to journalArticle

TY - JOUR

T1 - The Role of Natural Medicines on Wound Healing

T2 - A Biomechanical, Histological, Biochemical and Molecular Study

AU - Kotian, Sushma

AU - Bhat, Kumar

AU - Pai, Sreedhara

AU - Nayak, Jayakrishna

AU - Souza, Anne

AU - Gourisheti, Karthik

AU - Padma, Divya

PY - 2018/11/1

Y1 - 2018/11/1

N2 - Background: Traditional medicinal systems like Ayurveda and Indian folk medicine have used Honey, Ghee, Glycyrrhiza glabra, and Nerium indicum effectively for treating wounds. The known result of these medications is faster healing. However, the mechanism of actions at the tissue level, the biochemical and molecular mechanisms of healing is not well explored and documented. This present study was therefore designed to study the efficacy of these traditional medicines singly and in combinations on excision wounds in Wistar rats. Methods: At two different intervals (i.e., day 8 and day 16), biomechanical, histological and immunohistochemical (IHC) parameters were assessed at the wound site. IHC focused on the inflammatory rate by evaluating the level of cytokine, IL1β and the tissue remodeling by studying the activity of myofibroblasts. Results: Rapid epithelization, better remodeling, favorable inflammatory changes and an adequate myofibroblast activity at the wound site was observed in all the treated groups compared to control. Conclusion: This study is therefore useful in exploring the mechanism of action of these traditional medicines and providing valuable scientific evidence.

AB - Background: Traditional medicinal systems like Ayurveda and Indian folk medicine have used Honey, Ghee, Glycyrrhiza glabra, and Nerium indicum effectively for treating wounds. The known result of these medications is faster healing. However, the mechanism of actions at the tissue level, the biochemical and molecular mechanisms of healing is not well explored and documented. This present study was therefore designed to study the efficacy of these traditional medicines singly and in combinations on excision wounds in Wistar rats. Methods: At two different intervals (i.e., day 8 and day 16), biomechanical, histological and immunohistochemical (IHC) parameters were assessed at the wound site. IHC focused on the inflammatory rate by evaluating the level of cytokine, IL1β and the tissue remodeling by studying the activity of myofibroblasts. Results: Rapid epithelization, better remodeling, favorable inflammatory changes and an adequate myofibroblast activity at the wound site was observed in all the treated groups compared to control. Conclusion: This study is therefore useful in exploring the mechanism of action of these traditional medicines and providing valuable scientific evidence.

UR - http://www.scopus.com/inward/record.url?scp=85059502091&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059502091&partnerID=8YFLogxK

U2 - 10.4314/ejhs.v28i6.11

DO - 10.4314/ejhs.v28i6.11

M3 - Article

VL - 28

SP - 759

EP - 770

JO - Ethiopian journal of health sciences

JF - Ethiopian journal of health sciences

SN - 1029-1857

IS - 6

ER -