The significance of the secretory immunoglogulin A to Gardnerella vaginalis in the pathogenesis and diagnosis of bacterial vaginosis

Udayalaxmi, Gopalkrishna Bhat, Subannayya Kotigadde

Research output: Contribution to journalArticle

Abstract

Background: Although bacterial vaginosis is prevalent, not much progress has occurred in identifying the factors which are responsible and are associated with bacterial vaginosis and its pathophysiology. Studies on the development of better methods for the diagnosis of bacterial vaginosis are still on. Objective: To estimate the levels of secretory immunoglobulin A (sIgA) antibodies against G.vaginalis in vaginal discharge and its correlation with the clinical condition. Materials and Methods: We developed an indirect ELISA by using whole cell antigen to detect sIgA antibodies against G.vaginalis in vaginal washings which were collected from 170 women who attended two hospitals in south India for antenatal care or any other complaint. The women were categorised into bacterial vaginosis, intermediate and normal by using Nugent's criteria. Statistical analysis was done by using the Chi- Square test, analysis of variance and Scheffe's multiple range tests, as appropriate. Results: Of the 170 women under study, 42 were cases of bacterial vaginosis, 30 had intermediate flora and 98 were normal women. Of the 42 women with bacterial vaginosis, 16 (38%) showed a significantly high titre and 26 (62%) had low titre. Of the 30 women with intermediate flora, 10 (33.3%) showed a significantly high titre and 20 (66.7%) had low titres of the 98 women with normal flora, 84 (85.7%) showed low titres and 14 (14.3%) showed high titres of sIgA antibodies against G.vaginalis. The results of the ELISA test correlated statistically with the cases of bacterial vaginosis when analysed by Chi-Square test (p < 0.05). Analysis of variance showed that there was significant variation (p < 0.05) between the titers of sIgA antibodies detected in the women categorised by Nugent's criteria. Conclusion: All the women with bacterial vaginosis or with intermediate flora do not show significant titres of sIgA antibodies to G.vaginalis. So, the detection of sIgA to G.vaginalis in the vaginal discharge cannot be used as a means for the diagnosis of bacterial vaginosis.

Original languageEnglish
Pages (from-to)752-754
Number of pages3
JournalJournal of Clinical and Diagnostic Research
Volume5
Issue number4
Publication statusPublished - 2011

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Gardnerella vaginalis
Bacterial Vaginosis
Secretory Immunoglobulin A
Antibodies
Analysis of variance (ANOVA)
Vaginal Discharge
Chi-Square Distribution
Analysis of Variance
Enzyme-Linked Immunosorbent Assay
Washing
Statistical methods
Prenatal Care
Antigens
India

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Clinical Biochemistry

Cite this

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title = "The significance of the secretory immunoglogulin A to Gardnerella vaginalis in the pathogenesis and diagnosis of bacterial vaginosis",
abstract = "Background: Although bacterial vaginosis is prevalent, not much progress has occurred in identifying the factors which are responsible and are associated with bacterial vaginosis and its pathophysiology. Studies on the development of better methods for the diagnosis of bacterial vaginosis are still on. Objective: To estimate the levels of secretory immunoglobulin A (sIgA) antibodies against G.vaginalis in vaginal discharge and its correlation with the clinical condition. Materials and Methods: We developed an indirect ELISA by using whole cell antigen to detect sIgA antibodies against G.vaginalis in vaginal washings which were collected from 170 women who attended two hospitals in south India for antenatal care or any other complaint. The women were categorised into bacterial vaginosis, intermediate and normal by using Nugent's criteria. Statistical analysis was done by using the Chi- Square test, analysis of variance and Scheffe's multiple range tests, as appropriate. Results: Of the 170 women under study, 42 were cases of bacterial vaginosis, 30 had intermediate flora and 98 were normal women. Of the 42 women with bacterial vaginosis, 16 (38{\%}) showed a significantly high titre and 26 (62{\%}) had low titre. Of the 30 women with intermediate flora, 10 (33.3{\%}) showed a significantly high titre and 20 (66.7{\%}) had low titres of the 98 women with normal flora, 84 (85.7{\%}) showed low titres and 14 (14.3{\%}) showed high titres of sIgA antibodies against G.vaginalis. The results of the ELISA test correlated statistically with the cases of bacterial vaginosis when analysed by Chi-Square test (p < 0.05). Analysis of variance showed that there was significant variation (p < 0.05) between the titers of sIgA antibodies detected in the women categorised by Nugent's criteria. Conclusion: All the women with bacterial vaginosis or with intermediate flora do not show significant titres of sIgA antibodies to G.vaginalis. So, the detection of sIgA to G.vaginalis in the vaginal discharge cannot be used as a means for the diagnosis of bacterial vaginosis.",
author = "Udayalaxmi and Gopalkrishna Bhat and Subannayya Kotigadde",
year = "2011",
language = "English",
volume = "5",
pages = "752--754",
journal = "Journal of Clinical and Diagnostic Research",
issn = "2249-782X",
publisher = "Journal of Clinical and Diagnostic Research",
number = "4",

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T1 - The significance of the secretory immunoglogulin A to Gardnerella vaginalis in the pathogenesis and diagnosis of bacterial vaginosis

AU - Udayalaxmi,

AU - Bhat, Gopalkrishna

AU - Kotigadde, Subannayya

PY - 2011

Y1 - 2011

N2 - Background: Although bacterial vaginosis is prevalent, not much progress has occurred in identifying the factors which are responsible and are associated with bacterial vaginosis and its pathophysiology. Studies on the development of better methods for the diagnosis of bacterial vaginosis are still on. Objective: To estimate the levels of secretory immunoglobulin A (sIgA) antibodies against G.vaginalis in vaginal discharge and its correlation with the clinical condition. Materials and Methods: We developed an indirect ELISA by using whole cell antigen to detect sIgA antibodies against G.vaginalis in vaginal washings which were collected from 170 women who attended two hospitals in south India for antenatal care or any other complaint. The women were categorised into bacterial vaginosis, intermediate and normal by using Nugent's criteria. Statistical analysis was done by using the Chi- Square test, analysis of variance and Scheffe's multiple range tests, as appropriate. Results: Of the 170 women under study, 42 were cases of bacterial vaginosis, 30 had intermediate flora and 98 were normal women. Of the 42 women with bacterial vaginosis, 16 (38%) showed a significantly high titre and 26 (62%) had low titre. Of the 30 women with intermediate flora, 10 (33.3%) showed a significantly high titre and 20 (66.7%) had low titres of the 98 women with normal flora, 84 (85.7%) showed low titres and 14 (14.3%) showed high titres of sIgA antibodies against G.vaginalis. The results of the ELISA test correlated statistically with the cases of bacterial vaginosis when analysed by Chi-Square test (p < 0.05). Analysis of variance showed that there was significant variation (p < 0.05) between the titers of sIgA antibodies detected in the women categorised by Nugent's criteria. Conclusion: All the women with bacterial vaginosis or with intermediate flora do not show significant titres of sIgA antibodies to G.vaginalis. So, the detection of sIgA to G.vaginalis in the vaginal discharge cannot be used as a means for the diagnosis of bacterial vaginosis.

AB - Background: Although bacterial vaginosis is prevalent, not much progress has occurred in identifying the factors which are responsible and are associated with bacterial vaginosis and its pathophysiology. Studies on the development of better methods for the diagnosis of bacterial vaginosis are still on. Objective: To estimate the levels of secretory immunoglobulin A (sIgA) antibodies against G.vaginalis in vaginal discharge and its correlation with the clinical condition. Materials and Methods: We developed an indirect ELISA by using whole cell antigen to detect sIgA antibodies against G.vaginalis in vaginal washings which were collected from 170 women who attended two hospitals in south India for antenatal care or any other complaint. The women were categorised into bacterial vaginosis, intermediate and normal by using Nugent's criteria. Statistical analysis was done by using the Chi- Square test, analysis of variance and Scheffe's multiple range tests, as appropriate. Results: Of the 170 women under study, 42 were cases of bacterial vaginosis, 30 had intermediate flora and 98 were normal women. Of the 42 women with bacterial vaginosis, 16 (38%) showed a significantly high titre and 26 (62%) had low titre. Of the 30 women with intermediate flora, 10 (33.3%) showed a significantly high titre and 20 (66.7%) had low titres of the 98 women with normal flora, 84 (85.7%) showed low titres and 14 (14.3%) showed high titres of sIgA antibodies against G.vaginalis. The results of the ELISA test correlated statistically with the cases of bacterial vaginosis when analysed by Chi-Square test (p < 0.05). Analysis of variance showed that there was significant variation (p < 0.05) between the titers of sIgA antibodies detected in the women categorised by Nugent's criteria. Conclusion: All the women with bacterial vaginosis or with intermediate flora do not show significant titres of sIgA antibodies to G.vaginalis. So, the detection of sIgA to G.vaginalis in the vaginal discharge cannot be used as a means for the diagnosis of bacterial vaginosis.

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