Therapeutic potential of human bone marrow derived mesenchymal stromal cells in combination with silymarin against carbon tetrachloride induced liver cirrhosis in wistar rats

Ashwini P. Aithal, Laxminarayana K. Bairy, Raviraja N. Seetharam, Naveen Kumar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone Marrow derived Mesenchymal Stromal Cells (BM-MSC) and silymarin in Carbon tetrachloride (CCl4) induced liver cirrhosis in Wistar rats. Material and Methods: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl4. Sixty rats were randomly divided into 6 groups: Group 1 (Normal Control Group), Group 2 (received only CCl4), Group 3 (CCl4+low dose BM-MSCs), Group 4 (CCl4+high dose BM-MSCs), Group 5 (CCl4+ silymarin), Group 6 (CCl4+BM-MSCs+silymarin). On day 0 and at the end of 6th, 12th, 18th, 24th and 30th day after treatment, blood samples were collected for liver enzyme estimations. After 30 days of treatment, the rats were sacrificed; livers were excised and used for antioxidant analysis and histopathological studies. Results: Liver enzyme analysis and histopathological studies indicated that combination of BM-MSCs and silymarin was effective in treating liver cirrhosis. Furthermore, oxidative stress was attenuated in the group which received combination treatment of BM-MSCs and silymarin. Conclusion: Evidence of this study showed that combination treatment of BM- MSCs and silymarin was beneficial and support the potential of using MSCs transplantation as an effective treatment modality for liver cirrhosis.

Original languageEnglish
Pages (from-to)38-49
Number of pages12
JournalJournal of Krishna Institute of Medical Sciences University
Volume6
Issue number4
Publication statusPublished - 01-10-2017

Fingerprint

Silymarin
Carbon Tetrachloride
Mesenchymal Stromal Cells
Liver Cirrhosis
Wistar Rats
Bone Marrow
Liver
Therapeutics
Enzymes
Oxidative Stress
Antioxidants
Transplantation
Control Groups

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

@article{2a8c88c1228a4ff5a17ae12b4754f242,
title = "Therapeutic potential of human bone marrow derived mesenchymal stromal cells in combination with silymarin against carbon tetrachloride induced liver cirrhosis in wistar rats",
abstract = "Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone Marrow derived Mesenchymal Stromal Cells (BM-MSC) and silymarin in Carbon tetrachloride (CCl4) induced liver cirrhosis in Wistar rats. Material and Methods: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl4. Sixty rats were randomly divided into 6 groups: Group 1 (Normal Control Group), Group 2 (received only CCl4), Group 3 (CCl4+low dose BM-MSCs), Group 4 (CCl4+high dose BM-MSCs), Group 5 (CCl4+ silymarin), Group 6 (CCl4+BM-MSCs+silymarin). On day 0 and at the end of 6th, 12th, 18th, 24th and 30th day after treatment, blood samples were collected for liver enzyme estimations. After 30 days of treatment, the rats were sacrificed; livers were excised and used for antioxidant analysis and histopathological studies. Results: Liver enzyme analysis and histopathological studies indicated that combination of BM-MSCs and silymarin was effective in treating liver cirrhosis. Furthermore, oxidative stress was attenuated in the group which received combination treatment of BM-MSCs and silymarin. Conclusion: Evidence of this study showed that combination treatment of BM- MSCs and silymarin was beneficial and support the potential of using MSCs transplantation as an effective treatment modality for liver cirrhosis.",
author = "Aithal, {Ashwini P.} and Bairy, {Laxminarayana K.} and Seetharam, {Raviraja N.} and Naveen Kumar",
year = "2017",
month = "10",
day = "1",
language = "English",
volume = "6",
pages = "38--49",
journal = "Journal of Krishna Institute of Medical Sciences University",
issn = "2231-4261",
publisher = "Krishna Institute of Medical Sciences University",
number = "4",

}

TY - JOUR

T1 - Therapeutic potential of human bone marrow derived mesenchymal stromal cells in combination with silymarin against carbon tetrachloride induced liver cirrhosis in wistar rats

AU - Aithal, Ashwini P.

AU - Bairy, Laxminarayana K.

AU - Seetharam, Raviraja N.

AU - Kumar, Naveen

PY - 2017/10/1

Y1 - 2017/10/1

N2 - Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone Marrow derived Mesenchymal Stromal Cells (BM-MSC) and silymarin in Carbon tetrachloride (CCl4) induced liver cirrhosis in Wistar rats. Material and Methods: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl4. Sixty rats were randomly divided into 6 groups: Group 1 (Normal Control Group), Group 2 (received only CCl4), Group 3 (CCl4+low dose BM-MSCs), Group 4 (CCl4+high dose BM-MSCs), Group 5 (CCl4+ silymarin), Group 6 (CCl4+BM-MSCs+silymarin). On day 0 and at the end of 6th, 12th, 18th, 24th and 30th day after treatment, blood samples were collected for liver enzyme estimations. After 30 days of treatment, the rats were sacrificed; livers were excised and used for antioxidant analysis and histopathological studies. Results: Liver enzyme analysis and histopathological studies indicated that combination of BM-MSCs and silymarin was effective in treating liver cirrhosis. Furthermore, oxidative stress was attenuated in the group which received combination treatment of BM-MSCs and silymarin. Conclusion: Evidence of this study showed that combination treatment of BM- MSCs and silymarin was beneficial and support the potential of using MSCs transplantation as an effective treatment modality for liver cirrhosis.

AB - Background: Self-renewal, active proliferation in vitro, abundant sources for isolation, and a high differentiation capacity, make mesenchymal stem cells to be potentially therapeutic for liver cirrhosis. Aim and Objectives: To evaluate the therapeutic potential of the combination of human Bone Marrow derived Mesenchymal Stromal Cells (BM-MSC) and silymarin in Carbon tetrachloride (CCl4) induced liver cirrhosis in Wistar rats. Material and Methods: This was an experimental study. Liver cirrhosis was induced in adult female Wistar rats using CCl4. Sixty rats were randomly divided into 6 groups: Group 1 (Normal Control Group), Group 2 (received only CCl4), Group 3 (CCl4+low dose BM-MSCs), Group 4 (CCl4+high dose BM-MSCs), Group 5 (CCl4+ silymarin), Group 6 (CCl4+BM-MSCs+silymarin). On day 0 and at the end of 6th, 12th, 18th, 24th and 30th day after treatment, blood samples were collected for liver enzyme estimations. After 30 days of treatment, the rats were sacrificed; livers were excised and used for antioxidant analysis and histopathological studies. Results: Liver enzyme analysis and histopathological studies indicated that combination of BM-MSCs and silymarin was effective in treating liver cirrhosis. Furthermore, oxidative stress was attenuated in the group which received combination treatment of BM-MSCs and silymarin. Conclusion: Evidence of this study showed that combination treatment of BM- MSCs and silymarin was beneficial and support the potential of using MSCs transplantation as an effective treatment modality for liver cirrhosis.

UR - http://www.scopus.com/inward/record.url?scp=85030840586&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030840586&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 38

EP - 49

JO - Journal of Krishna Institute of Medical Sciences University

JF - Journal of Krishna Institute of Medical Sciences University

SN - 2231-4261

IS - 4

ER -